1921
Volume 96, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Environmental enteric dysfunction (EED) is often measured with a dual sugar absorption test and implicated as a causative factor in childhood stunting. Disturbances in the gut microbiota are hypothesized to be a mechanism by which EED is exacerbated, although this supposition lacks support. We performed 16S ribosomal RNA gene sequencing of fecal samples from 81 rural Malawian children with varying degrees of EED to determine which bacterial taxa were associated with EED. At the phyla level, Proteobacteria abundance is reduced with severe EED. Among bacterial genera, , , and were higher in EED and , , and were lower in EED. Bacterial diversity did not vary with the extent of EED. Though EED is a condition that is typically believed to affect the proximal small bowel, and our focus was on stool, our data do suggest that there are intraluminal microbial differences that reflect, or plausibly lead to, EED.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.16-0617
2017-02-08
2018-09-26
Loading full text...

Full text loading...

/deliver/fulltext/14761645/96/2/473.html?itemId=/content/journals/10.4269/ajtmh.16-0617&mimeType=html&fmt=ahah

References

  1. Keusch GT, Denno DM, Black RE, Duggan C, Guerrant RL, Lavery JV, Nataro JP, Rosenberg IH, Ryan ET, Tarr PI, Ward H, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AK, Hay Burgess DC, Brewer T, , 2014. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Clin Infect Dis 59 (Suppl 4): S207S212.[Crossref]
  2. Prendergast AJ, Kelly P, , 2016. Interactions between intestinal pathogens, enteropathy and malnutrition in developing countries. Curr Opin Infect Dis 29: 229236.[Crossref]
  3. Denno DM, VanBuskirk K, Nelson ZC, Musser CA, Hay Burgess DC, Tarr PI, , 2014. Use of the lactulose to mannitol ratio to evaluate childhood environmental enteric dysfunction: a systematic review. Clin Infect Dis 59 (Suppl 4): S213S219.[Crossref]
  4. Trehan I, Benzoni NS, Wang AZ, Bollinger LB, Ngoma TN, Chimimba UK, Stephenson KB, Agapova SE, Maleta KM, Manary MJ, , 2015. Common beans and cowpeas as complementary foods to reduce environmental enteric dysfunction and stunting in Malawian children: study protocol for two randomized controlled trials. Trials 16: 520.[Crossref]
  5. Stauber J, Shaikh N, Ordiz MI, Tarr PI, Manary MJ, , 2016. Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly. Cell Immunol 303: 4349.[Crossref]
  6. Ordiz MI, Shaikh N, Trehan I, Maleta K, Stauber J, Shulman R, Devaraj S, Tarr PI, Manary MJ, , 2016. Environmental enteric dysfunction is associated with poor linear growth and can be identified by host fecal mRNAs. J Pediatr Gastroenterol Nutr 63: 453459.[Crossref]
  7. Ordiz MI, May TD, Mihindukulasuriya K, Martin J, Crowley J, Tarr PI, Ryan K, Mortimer E, Gopalsamy G, Maleta K, Mitreva M, Young G, Manary MJ, , 2015. The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children. Microbiome 3: 37.[Crossref]
  8. Schloss PD, , 2009. A high-throughput DNA sequence aligner for microbial ecology studies. PLoS One 4: e8230.[Crossref]
  9. Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methé B, DeSantis TZ, Human Microbiome Consortium Petrosino JF, Knight R, Birren BW, ; , , 2011. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21: 494504.[Crossref]
  10. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM, , 2009. The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37: D141D145.[Crossref]
  11. Oksanen J, Blanchet FG, Kindt R, Legendre P, O'Hara RB, Simpson GL, Solymos P, Henry M, Stevens H, Wagner H, , 2016. Vegan: Community Ecology Package 2016. Available at: https://CRAN.R-project.org/package=vegan.
  12. Verdu EF, Galipeau HJ, Jabri B, , 2015. Novel players in coeliac disease pathogenesis: role of the gut microbiota. Nat Rev Gastroenterol Hepatol 12: 497506.[Crossref]
  13. Graf D, Di Cagno R, Fåk F, Flint HJ, Nyman M, Saarela M, Watzl B, , 2015. Contribution of diet to the composition of the human gut microbiota. Microb Ecol Health Dis 26: 26164.
  14. Ridlon JM, Kang DJ, Hylemon PB, , 2006. Bile salt biotransformations by human intestinal bacteria. J Lipid Res 47: 241259.[Crossref]
  15. Semba RD, Gonzalez-Freire M, Moaddel R, Trehan I, Maleta K, Khadeer M, Ordiz MI, Ferrucci L, Manary MJ, , 2016. Environmental enteric dysfunction is associated with altered bile acid metabolism. J Pediatr Gastroenterol Nutr. doi:10.1097/MPG.0000000000001313.
  16. Marounek M, Fliegrova K, Bartos S, , 1989. Metabolism and some characteristics of ruminal strains of Megasphaera elsdenii . Appl Environ Microbiol 55: 15701573.
  17. Wacklin P, Kaukinen K, Tuovinen E, Collin P, Lindfors K, Partanen J, Mäki M, Mättö J, , 2013. The duodenal microbiota composition of adult celiac disease patients is associated with the clinical manifestation of the disease. Inflamm Bowel Dis 19: 934941.[Crossref]
  18. Vázquez-Castellanos JF, Serrano-Villar S, Latorre A, Artacho A, Ferrús ML, Madrid N, Vallejo A, Sainz T, Martínez-Botas J, Ferrando-Martínez S, Vera M, Dronda F, Leal M, Del Romero J, Moreno S, Estrada V, Gosalbes MJ, Moya A, , 2015. Altered metabolism of gut microbiota contributes to chronic immune activation in HIV-infected individuals. Mucosal Immunol 8: 760772.[Crossref]
  19. Levine UY, Bearson SMD, Stanton TB, , 2012. Mitsuokella jalaludinii inhibits growth of Salmonella enterica serovar Typhimurium. Veterinarian Microbiology 159: 115122.[Crossref]
  20. Mangin I, Bonnet R, Seksik P, Rigottier-Gois L, Sutren M, Bouhnik Y, Neut C, Collins MD, Colombel JF, Marteau P, Doré J, , 2004. Molecular inventory of faecal microflora in patients with Crohn's disease. FEMS Microbiol Ecol 50: 2536.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.16-0617
Loading
/content/journals/10.4269/ajtmh.16-0617
Loading

Data & Media loading...

  • Received : 30 Jul 2016
  • Accepted : 23 Oct 2016

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error