1921
Volume 95, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The emergence of artemisinin resistance among in the Greater Mekong subregion threatens malaria control interventions and is associated with multiple unique mutations in (PF3D7_1343700). The aim of this study was to survey Cambodian for mutations in the ortholog (, PVX_083080) that might similarly confer artemisinin resistance. Extracted DNA from Cambodian isolates collected between 2009 and 2012 was pooled by province and year and submitted for next-generation sequencing. Single-nucleotide polymorphisms (SNPs) were identified using a pile-up approach that detected minority SNPs. Among the 14 pools, we found six unique SNPs, including three nonsynonymous SNPs, across six codons in . However, none of the SNPs were orthologous to artemisinin resistance–conferring mutations in PF3D7_1343700, and nonsynonymous changes did not persist through time within populations. These results suggest a lack of selection in the population in Cambodia due to artemisinin drug pressure.

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/content/journals/10.4269/ajtmh.16-0566
2016-12-07
2017-11-19
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Supplementary Data

Supplementary PDF

  • Received : 09 Jul 2016
  • Accepted : 30 Aug 2016

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