1921
Volume 97, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The four serotypes of dengue virus (DENV-1, -2, -3, and -4) have had a rapidly expanding geographic range and are now endemic in over 100 tropical and subtropical countries. Sri Lanka has experienced periodic dengue outbreaks since the 1960s, but since 1989 epidemics have become progressively larger and associated with more severe disease. The dominant virus in the 2012 epidemic was DENV-1, but DENV-4 infections were also commonly observed. DENV-4 transmission was first documented in Sri Lanka when it was isolated from a traveler in 1978, but has been comparatively uncommon since dengue surveillance began in the early 1980s. To better understand the molecular epidemiology of DENV-4 infections in Sri Lanka, we conducted whole-genome sequencing on dengue patient samples from two different geographic locations. Phylogenetic analysis indicates that all sequenced DENV-4 strains belong to genotype 1 and are most closely related to DENV-4 viruses previously found in Sri Lanka and those recently found to be circulating in India and Pakistan.

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2017-07-12
2018-07-19
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References

  1. Gubler DJ, , 1997. Epidemic dengue/dengue haemorrhagic fever: a global public health problem in the 21st century. Dengue Bull 21: 114.
  2. World Health Organization, 2012. Global Strategy for Dengue Prevention and Control 2012–2020. Geneva, Switzerland: World Health Organization Press.
  3. Wilder-Smith A1, Gubler DJ, , 2008. Geographic expansion of dengue: the impact of international travel. Med Clin North Am 92: 13771390.[Crossref]
  4. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI, , 2013. The global distribution and burden of dengue. Nature 496: 504507.[Crossref]
  5. Beatty ME, Beutels P, Meltzer MI, Shepard DS, Hombach J, Hutubessy R, Dessis D, Coudeville L, Dervaux B, Wichmann O, Margolis HS, Kuritsky JN, , 2011. Health economics of dengue: a systematic literature review and expert panel’s assessment. Am J Trop Med Hyg 84: 473488.[Crossref]
  6. Shepard DS, Undurraga EA, Betancourt-Cravioto M, Guzman MG, Halstead SB, Harris E, Mudin RN, Murray KO, Tapia-Conyer R, Gubler DJ, , 2014. Approaches to refining estimates of global burden and economics of dengue. PLoS Negl Trop Dis 8: e3306.[Crossref]
  7. Gubler DJ, , 2011. Dengue, urbanization and globalization: the Unholy Trinity of the 21(st) century. Trop Med Health 39: 311.[Crossref]
  8. Vitarana T, Jayakuru WS, Withane N, , 1997. Historical account of dengue haemorrhagic fever in Sri Lanka. Dengue Bull 21: 2.
  9. Messer WB, Vitarana UT, Sivananthan K, Elvtigala J, Preethimala LD, Ramesh R, Withana N, Gubler DJ, De Silva AM, , 2002. Epidemiology of dengue in Sri Lanka before and after the emergence of epidemic dengue hemorrhagic fever. Am J Trop Med Hyg 66: 765773.[Crossref]
  10. Lanciotti RS, Gubler DJ, Trent DW, , 1997. Molecular evolution and phylogeny of dengue-4 viruses. J Gen Virol 78: 22792284.[Crossref]
  11. Lanciotti RS, Lewis JG, Gubler DJ, Trent DW, , 1994. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75: 6575.[Crossref]
  12. Kanakaratne N, Wahala WMPB, Messer WB, Tissera HA, Shahani A, Abeysinghe N, de Silva AM, Gunasekera M, , 2009. Severe dengue epidemics in Sri Lanka, 2003–2006. Emerg Infect Dis 15: 192199.[Crossref]
  13. Thalagala N, Tissera H, Palihawadana P, Amarasinghe A, Ambagahawita A, Wilder-Smith A, Shepard DS, Tozan Y, , 2016. Costs of dengue control activities and hospitalizations in the public health sector during an epidemic year in urban Sri Lanka. PLoS Negl Trop Dis 10: e0004466.[Crossref]
  14. Messer WB, Gubler DJ, Harris E, Sivananthan K, de Silva AM, , 2003. Emergence and global spread of a dengue serotype 3, subtype III virus. Emerg Infect Dis 9: 800809.[Crossref]
  15. Tissera HA, Ooi EE, Gubler DJ, Tan Y, Logendra B, Wahala WM, de Silva AM, Abeysinghe MR, Palihawadana P, Gunasena S, Tam CC, Amarasinghe A, Letson GW, Margolis HS, De Silva AD, , 2011. New dengue virus type 1 genotype in Colombo, Sri Lanka. Emerg Infect Dis 17: 20532055.[Crossref]
  16. Health EUoMo, Disease Surveillance. Available at: http://www.epid.gov.lk/web/index.php. Accessed February 16, 2013.
  17. Initiative DV, , 2015. Dengue in Sri Lanka: Burden, Challenges and Prevention Strategies–An Exclusive Interview with Dr. Hasitha Tissera. Available at: http://www.denguevaccines.org/dengue-sri-lanka-burden-challenges-and-prevention-strategies-exclusive-interview-dr-hasitha-tissera. Accessed November 17, 2016.
  18. Tissera H, Amarasinghe A, Gunasena S, DeSilva AD, Yee LW, Sessions O, Muthukuda C, Palihawadana P, Lohr W, Byass P, Gubler DJ, Wilder-Smith A, , 2016. Laboratory-enhanced dengue sentinel surveillance in Colombo District, Sri Lanka: 2012–2014. PLoS Negl Trop Dis 10: e0004477.[Crossref]
  19. Bodinayake CK, Tillekeratne LG, Nagahawatte A, Devasiri V, Kodikara Arachichi W, Strouse JJ, Sessions OM, Kurukulasooriya R, Uehara A, Howe S, Ong XM, Tan S, Chow A, Tummalapalli P, De Silva AD, Østbye T, Woods CW, Gubler DJ, Reller ME, , 2016. Emergence of epidemic dengue-1 virus in the southern province of Sri Lanka. PLoS Negl Trop Dis 10: e0004995.[Crossref]
  20. Christenbury JG, Aw PP, Ong SH, Schreiber MJ, Chow A, Gubler DJ, Vasudevan SG, Ooi EE, Hibberd ML, , 2010. A method for full genome sequencing of all four serotypes of the dengue virus. J Virol Methods 169: 202206.[Crossref]
  21. Sanger F, Nicklen S, Coulson AR, , 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 54635467.[Crossref]
  22. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P, , 2010. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26: 24622463.[Crossref]
  23. Edgar RC, , 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 17921797.[Crossref]
  24. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O, , 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59: 307321.[Crossref]
  25. Darriba D, Taboada GL, Doallo R, Posada D, , 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9: 772.[Crossref]
  26. Guindon S, Gascuel O, , 2003. A Simple, Fast and Accurate Method to Estimate Large Phylogenies by Maximum-Likelihood. Syst Biol 52: 696704.[Crossref]
  27. Drummond AJ, Suchard MA, Xie D, Rambaut A, , 2012. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 29: 19691973.[Crossref]
  28. Messina JP, Brady OJ, Scott TW, Zou C, Pigott DM, Duda KA, Bhatt S, Katzelnick L, Howes RE, Battle KE, Simmons CP, Hay SI, , 2014. Global spread of dengue virus types: mapping the 70 year history. Trends Microbiol 22: 138146.[Crossref]
  29. Steel A, Gubler DJ, Bennett SN, , 2010. Natural attenuation of dengue virus type-2 after a series of island outbreaks: a re-trospective phylogenetic study of events in the South Pacific three decades ago. Virology 405: 8.[Crossref]
  30. Bennett SN, Holmes E, Chirivella M, Rodriguez DM, Beltran M, Vorndam V, Gubler DJ, McMillan WO, , 2006. Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction. J Gen Virol 87: 9.[Crossref]
  31. Manokaran G, Finol E, Wang C, Gunaratne J, Bahl J, Ong EZ, Tan HC, Sessions OM, Ward AM, Gubler DJ, Harris E, Garcia-Blanco MA, Ooi EE, , 2015. Dengue subgenomic RNA binds TRIM25 to inhibit interferon expression for epidemiological fitness. Science 350: 5.[Crossref]
  32. Aw PP, de Sessions PF, Wilm A, Hoang LT, Nagarajan N, Sessions OM, Hibberd ML, , 2014. Next-generation whole genome sequencing of dengue virus. Methods Mol Biol 1138: 175195.[Crossref]
  33. Tang Y, Rodpradit P, Chinnawirotpisan P, Mammen MP, Li T, Lynch JA, Putnak R, Zhang C, , 2010. Comparative analysis of full-length genomic sequences of 10 dengue serotype 1 viruses associated with different genotypes, epidemics, and disease severity isolated in Thailand over 22 years. Am J Trop Med Hyg 83: 11561165.[Crossref]
  34. Tissera H, Amarasinghe A, De Silva AD, Kariyawasam P, Corbett KS, Katzelnick L, Tam C, Letson GW, Margolis HS, de Silva AM, , 2014. Burden of dengue infection and disease in a pediatric cohort in urban Sri Lanka. Am J Trop Med Hyg 91: 132137.[Crossref]
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Supplementary Data

Supplemental Figure and Table

  • Received : 30 Jun 2016
  • Accepted : 26 Jan 2017

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