1921
Volume 97, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We report the first isolation of in South Korea. A 61-year-old woman presented with a 6-day history of fever, headache, and myalgia. Initial investigation showed neutropenia and thrombocytopenia. We diagnosed human granulocytic anaplasmosis by microscopic examination and serologic testing. The patient recovered fully without antibiotic therapy. The isolate was obtained from the patient’s blood by cell culture and mouse inoculation. Its identity was confirmed by an immunofluorescence assay, sequencing of the 16S rRNA gene, (), and genes, and staining and electron microscopy of morulae of in cultured human promyelocytic leukemia HL-60 cells.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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References

  1. Chen SM, Dumler JS, Bakken JS, , Walker DH, 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human-disease. J Clin Microbiol 32: 589595. [Google Scholar]
  2. Dumler JS, Madigan JE, Pusterla N, , Bakken JS, 2007. Ehrlichioses in humans: epidemiology, clinical presentation, diagnosis, and treatment. Clin Infect Dis 45 (Suppl 1): S45S51. [Google Scholar]
  3. Bakken JS, Dumler JS, , 2000. Human granulocytic ehrlichiosis. Clin Infect Dis 31: 554560. [Google Scholar]
  4. Choi KS, Dumler JS, , 2013. Anaplasma phagocytophilum, interferon gamma production and Stat1 signaling. Microbiol Immunol 57: 207212. [Google Scholar]
  5. Park SW, Song BG, Shin EH, , Yun SM, Han MG, Park MY, Park C, Ryou J, 2014. Prevalence of severe fever with thrombocytopenia syndrome virus in Haemaphysalis longicornis ticks in South Korea. Ticks Tick Borne Dis 5: 975977. [Google Scholar]
  6. Kim CM, Kim MS, Park MS, , Park JH, Chae JS, 2003. Identification of Ehrlichia chaffeensis, Anaplasma phagocytophilum, and A. bovis in Haemaphysalis longicornis and Ixodes persulcatus ticks from Korea. Vector Borne Zoonotic Dis 3: 1726. [Google Scholar]
  7. Heo EJ, Park JH, Koo JR, , Park MS, Park MY, Dumler JS, Chae JS, 2002. Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients. J Clin Microbiol 40: 30823085. [Google Scholar]
  8. Park JH, Heo EJ, Choi KS, , Dumler JS, Chae JS, 2003. Detection of antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis antigens in sera of Korean patients by western immunoblotting and indirect immunofluorescence assays. Clin Diagn Lab Immunol 10: 10591064. [Google Scholar]
  9. Kim KH, Yi J, Oh WS, , Kim NH, Choi SJ, Choe PG, Kim NJ, Lee JK, Oh MD, 2014. Human granulocytic anaplasmosis, South Korea, 2013. Emerg Infect Dis 20: 17081711. [Google Scholar]
  10. Blas-Machado U, de la Fuente J, Blouin EF, , Almazán C, Kocan KM, Mysore JV, 2007. Experimental infection of C3H/HeJ mice with the NY18 isolate of Anaplasma phagocytophilum. Vet Pathol 44: 6473. [Google Scholar]
  11. Oh JY, Moon B-C, Bae BK, Shin E-H, Ko YH, Kim Y-J, Park YH, Chae J-S, 2009. Genetic identification and phylogenetic analysis of Anaplasma and Ehrlichia species in Haemaphysalis longicornis collected from Jeju Island, Korea. J Bacteriol Virol 39: 257267. [Google Scholar]
  12. Massung RF, Levin ML, Munderloh UG, Silverman DJ, Lynch MJ, Gaywee JK, Kurtti TJ, 2007. Isolation and propagation of the Ap-Variant 1 strain of Anaplasma phagocytophilum in a tick cell line. J Clin Microbiol 21382143. [Google Scholar]
  13. Zeidner NS, Burkot TR, Massung R, Nicholson WL, Dolan MC, Rutherford JS, Biggerstaff BJ, Maupin GO, 2000. Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks: evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in northern Colorado. J Infect Dis 182: 616619. [Google Scholar]
  14. Kang JG, Ko S, Kim YJ, , Yang HJ, Lee H, Shin NS, Choi KS, Chae JS, 2011. New genetic variants of Anaplasma phagocytophilum and Anaplasma bovis from Korean Water Deer (Hydropotes inermis argyropus). Vector Borne Zoonotic Dis 11: 929938. [Google Scholar]
  15. Rand JV, Tarasen AJ, Kumar J, , Homan SM, Tobin E, 2014. Intracytoplasmic granulocytic morulae counts on confirmed cases of ehrlichiosis/anaplasmosis in the northeast. Am J Clin Pathol 141: 683686. [Google Scholar]
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  • Received : 28 Jun 2016
  • Accepted : 01 May 2017
  • Published online : 30 Oct 2017

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