1921
Volume 95, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Babesiosis is an emerging tick-borne disease caused by apicomplexan parasites of the genus . Most human infections in the United States are caused by , but other infection-causing parasites have been documented as well. Polymerase chain reaction (PCR)–based methods can be used to identify this parasite to the species level. In this study, published real-time PCR assays for the specific detection of were evaluated against conventional PCR for their analytical performance. All evaluated real-time PCR assays had comparable dynamic range and amplification efficiency, but the sensitivity and specificity varied. The best performing test, a TaqMan assay targeting the 18S ribosomal RNA gene, was further evaluated for diagnostic performance using blood specimens submitted to the Centers for Disease Control and Prevention for parasite detection and was found to have 100% sensitivity and specificity. In conclusion, the 18S TaqMan real-time PCR assay is a sensitive, specific, and rapid method for identification of among cases of babesiosis in the United States.

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Supplementary PDF

  • Received : 19 May 2016
  • Accepted : 25 Aug 2016

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