1921
Volume 95, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

This study aimed to compare polymerase chain reaction (PCR) with serum agglutination test (SAT) in the diagnosis of patients before and 6 months after treatment. Peripheral blood specimens from 50 patients with brucellosis (case group) and 30 subjects without brucellosis (control group) were selected and entered into the study. The diagnosis of brucellosis was established using SAT ≥ 1:160 and 2-mercaptoethanol (2-ME) ≥ 1:80 with clinical signs and symptoms compatible with brucellosis. For each case, both before treatment and 6 months after completion of therapy, SAT, 2-ME, and PCR were performed. Subjects in the control group were assessed by the same tests at the initial visit. In the case group, 50 patients (36 males, 14 females) with the mean age of 43.6 ± 14.5 years were evaluated. The mean age of the control group was 40.6 ± 14 years. Among the 50 patients whose nested PCR assays were initially positive, 43 (86%) were negative 6 months after completing treatment. Relapse occurred in five (10%) patients within 6 months after treatment and all were PCR positive. None of the patients in the control group was PCR positive. Results show that PCR seems to be highly sensitive and specific, and therefore is a useful method for both the initial diagnosis and detection of relapse or chronic brucellosis.

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2016-12-07
2018-08-14
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References

  1. Christopher S, Umapathy BL, Ravikumar KL, , 2010. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. J Lab Physicians 2: 5560.[Crossref]
  2. Galinska EM, Zagórski J, , 2013. Brucellosis in humans: etiology, diagnostics, clinical forms. Ann Agric Environ Med 20: 233238.
  3. Pappas G, , 2008. Treatment of brucellosis. BMJ 336: 678679.[Crossref]
  4. Solera J, , 2010. Update on brucellosis: therapeutic challenges. Int J Antimicrob Agents 36: 1820.[Crossref]
  5. Araj GF, , 2010. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob Agents 36: 712.[Crossref]
  6. Nielsen K, Yu W, , 2010. Serological diagnosis of brucellosis. Prilozi 31: 6589.
  7. Pabuccuoglu O, Ecemis T, El S, Coskun A, Akcali S, Sanlidag T, , 2011. Evaluation of serological tests for diagnosis of brucellosis. Jpn J Infect Dis 64: 272276.
  8. Al Dahouk S, Nöckler K, , 2011. Implications of laboratory diagnosis on brucellosis therapy. Expert Rev Anti Infect Ther 9: 833845.[Crossref]
  9. Zamanian M, Hashemi Tabar GR, Rad M, Haghparast A, , 2015. Evaluation of different primers for detection of Brucella in human and animal serum samples by using PCR method. Arch Iran Med 18: 4450.
  10. Yu WL, Nielsen K, , 2010. Review of detection of Brucella spp. by polymerase chain reaction. Croat Med J 51: 306313.[Crossref]
  11. Asaad AM, Alqahtani JM, , 2012. Serological and molecular diagnosis of human brucellosis in Najran, southwestern Saudi Arabia. J Infect Public Health 5: 189194.[Crossref]
  12. Gemechu MY, Gill JPS, Arora AK, Ghatak S, Singh DK, , 2011. Polymerase chain reaction (PCR) assay for rapid diagnosis and its role in prevention of human brucellosis in Punjab, India. Int J Prev Med 2: 170177.
  13. Navarro E, Segura JC, Castaño MJ, Solera J, , 2006. Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin Infect Dis 42: 12661273.[Crossref]
  14. Al-Ajlan HH, Ibrahim AS, Al-Salamah AA, , 2011. Comparison of different PCR methods for detection of Brucella spp. in human blood samples. Pol J Microbiol 60: 2733.
  15. Navarro E, Escribano J, Fernandez J, Solera J, , 2002. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunol Med Microbiol 34: 147151.[Crossref]
  16. Cloeckaert A, Grayon M, Grepinet O, , 2000. An IS711 element downstream of the bp26 gene is a specific marker of Brucella spp. isolated from marine mammals. Clin Diagn Lab Immunol 7: 835839.
  17. Baily GG, Krahn JB, Drasar BS, Stoker NG, , 1992. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95: 271275.
  18. Bounaadja L, Albert D, Chenais B, Henault S, Zygmunt MS, Poliak S, Garin-Bastuji B, , 2009. Real-time PCR for identification of Brucella spp. a comparative study of IS711, bcsp31 and per target genes. Vet Microbiol 28: 156164.[Crossref]
  19. Hajia M, Fallah F, Angoti G, Karimi A, Rahbar M, Gachkar L, Mokhtar B, Sanaei A, Rastegar Lari A, , 2013. Comparison of methods for diagnosing brucellosis. Lab Med 44: 2933.[Crossref]
  20. Alikhani MY, Hashemi SH, Naseri Z, Farajnia S, Peeri-Dogaheh H, Hashemi S, , 2013. Diagnosis of human brucellosis by blood culture (BACTEC) and PCR method via whole blood and serum. Jundishapur J Microbiol 6: 248251.
  21. Wang Y, Wang Z, Zhang Y, Bai L, Zhao Y, Liu C, Ma A, Yu H, , 2014. Polymerase chain reaction–based assays for the diagnosis of human brucellosis. Ann Clin Microbiol Antimicrob 13: 31.[Crossref]
  22. Castaño MJ, Solera J, , 2009. Chronic brucellosis and persistence of Brucella melitensis DNA. J Clin Microbiol 47: 20842089.[Crossref]
  23. Vrioni G, Bourdakis A, Pappas G, Pitiriga V, Mavrouli M, Pournaras S, Tsakris A, , 2014. Administration of a triple versus a standard double antimicrobial regimen for human brucellosis more efficiently eliminates bacterial DNA load. Antimicrob Agents Chemother 58: 75417544.[Crossref]
  24. Roushan MR, Amiri MJ, Laly A, Mostafazadeh A, Bijani A, , 2010. Follow-up standard agglutination and 2-mercaptoethanol tests in 175 clinically cured cases of human brucellosis. Int J Infect Dis 14: 250253.[Crossref]
  25. Mohammad Hasani S, Mirnejad R, Amani J, Vafadar MJ, , 2016. Comparing rapid and specific detection of Brucella in clinical samples by PCR-ELISA and multiplex-PCR method. Iran J Pathol 11: 144150.
  26. Vrioni G, Pappas G, Priavali E, Gartzonika C, Levidiotou S, , 2008. An eternal microbe: Brucella DNA load persists for years after clinical cure. Clin Infect Dis 4612: 131136.[Crossref]
  27. Maas KS, Mendez M, Zavaleta M, Manrique J, Franco MP, Mulder M, Bonifacio N, Castaneda ML, Chacaltana J, Yagui E, Guillen A, Blazes DL, Espinosa B, Hall E, Abdoel TH, Smits HL, , 2007. Evaluation of brucellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up. Am J Trop Med Hyg 76: 698702.
  28. Al Dahouk S, Sprague LD, Neubauer H, , 2013. New developments in the diagnostic procedures for zoonotic brucellosis in humans. Rev Sci Tech 32: 177188.
  29. Kamal IH, Al Gashgari B, Moselhy SS, Kumosani TA, Abulnaja KO, , 2013. Two-stage PCR assay for detection of human brucellosis in endemic areas. BMC Infect Dis 13: 145.[Crossref]
  30. Al-Nakkas A, Mustafa AS, Wright SG, , 2005. Large-scale evaluation of a single-tube nested PCR for the laboratory diagnosis of human brucellosis in Kuwait. J Med Microbiol 54: 727730.[Crossref]
  31. Al Nakkas A, Wright S, Mustafa A, Wilson S, , 2002. Single-tube, nested PCR for the diagnosis of human brucellosis in Kuwait. Ann Trop Med Parasitol 96: 397403.[Crossref]
  32. Hassanain NA, Ahmed WM, , 2012. Efficacy of serological tests in comparison with PCR for diagnosis of brucellosis. World J Med Sci 7: 243247.
  33. Masallat D, Moemen D, Eid MI, , 2013. PCR versus serology for diagnosing of brucellosis in pregnant women: Mansoura University Hospital experience. Egypt J Med Microbiol 22: 133138.[Crossref]
  34. Khosravi AD, Abassi E, Alavi SM, , 2006. Isolation of Brucella melitensis and Brucella abortus from brucellosis patients by conventional culture method and polymerase chain reaction technique. Pak J Med Sci 22: 396400.
  35. Skendros P, Boura P, , 2013. Immunity to brucellosis. Rev Sci Tech 32: 137147.
  36. Kim EJ, Lee SJ, Ahn EY, Ryu DG, Choi YH, Kim TH, , 2015. Relapsed brucellosis presenting as neurobrucellosis with cerebral vasculitis in a patient previously diagnosed with Brucella spondylitis: a case report. Infect Chemother 47: 268271.[Crossref]
  37. Roushan MR, Amiri MS, Janmohammadi N, Hadad MS, Javanian M, Baiani M, Bijani A, , 2010. Comparison of the efficacy of gentamicin for 5 days plus doxycycline for 8 weeks versus streptomycin for 2 weeks plus doxycycline for 45 days in the treatment of human brucellosis: a randomized clinical trial. J Antimicrob Chemother 65: 10281035.[Crossref]
  38. Mitka S, Anetakis C, Souliou E, Diza E, Kansouzidou A, , 2007. Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods. J Clin Microbiol 45: 12111218.[Crossref]
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  • Received : 01 May 2016
  • Accepted : 12 Sep 2016

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