1921
Volume 95, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Portable microscopy may facilitate quality diagnostic care in resource-constrained settings. We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1–85.9%) and 100.0% (95% CI: 92.6–100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4–100.0%) and 65.6% (95% CI: 54.9–74.9%), respectively. If sensitivity can be improved, handheld light microscopy may become a valuable public health tool for diagnosis.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.16-0328
2016-10-05
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/14761645/95/4/831.html?itemId=/content/journals/10.4269/ajtmh.16-0328&mimeType=html&fmt=ahah

References

  1. WHO, 2012. T3: Test. Treat. and Track. Scaling Up Diagnostic Testing, Treatment and Surveillance of Malaria. Geneva, Switzerland: World Health Organization.
    [Google Scholar]
  2. WHO, 2011. Universal Access to Malaria Diagnostic Testing—An Operational Manual. November 2011 (rev. February 2013). Geneva, Switzerland: World Health Organization.
    [Google Scholar]
  3. Bell D, Wongsrichanalai C, Barnwell JW, 2006. Ensuring quality and access for malaria diagnosis: how can it be achieved? Nat Rev Microbiol 4 (Suppl): S7S20.[Crossref]
    [Google Scholar]
  4. Mouatcho JC, Goldring JPD, 2013. Malaria rapid diagnostic tests: challenges and prospects. J Med Microbiol 62: 14911505.[Crossref]
    [Google Scholar]
  5. Mundy C, Ngwira M, Kadewele G, Bates I, Squire SB, Gilks CF, 2000. Evaluation of microscope condition in Malawi. Trans R Soc Trop Med Hyg 94: 583584.[Crossref]
    [Google Scholar]
  6. Bates I, Bekoe V, Asamoa-Adu A, 2004. Improving the accuracy of malaria-related laboratory tests in Ghana. Malar J 3: 38.[Crossref]
    [Google Scholar]
  7. Petti CA, Polage CR, Quinn TC, Ronald AR, Sande MA, 2006. Laboratory medicine in Africa: a barrier to effective health care. Clin Infect Dis 42: 377382.[Crossref]
    [Google Scholar]
  8. Stothard JR, Kabatereine NB, Tukahebwa EM, Kazibwe F, Mathieson W, Webster JP, Fenwick A, 2005. Field evaluation of the Meade Readiview handheld microscope for diagnosis of intestinal schistosomiasis in Ugandan school children. Am J Trop Med Hyg 73: 949955.
    [Google Scholar]
  9. Bogoch II, Andrews JR, Speich B, Utzinger J, Ame SM, Ali SM, Keiser J, 2013. Mobile phone microscopy for the diagnosis of soil-transmitted helminth infections: a proof-of-concept study. Am J Trop Med Hyg 88: 626629.[Crossref]
    [Google Scholar]
  10. Bogoch II, Coulibaly JT, Andrews JR, Speich B, Keiser J, Stothard JR, N'Goran EK, Utzinger J, 2014. Evaluation of portable microscopic devices for the diagnosis of Schistosoma and soil-transmitted helminth infection. Parasitology 141: 18111818.[Crossref]
    [Google Scholar]
  11. Bogoch II, Andrews JR, Speich B, Ame SM, Ali SM, Stothard JR, Utzinger J, Keiser J, 2014. Quantitative evaluation of a handheld light microscope for field diagnosis of soil-transmitted helminth infection. Am J Trop Med Hyg 91: 11381141.[Crossref]
    [Google Scholar]
  12. Stothard JR, Nabatte B, Sousa-Figueiredo JC, Kabatereine NB, 2014. Towards malaria microscopy at the point-of-contact: an assessment of the diagnostic performance of the Newton Nm1 microscope in Uganda. Parasitology 141: 18191825.[Crossref]
    [Google Scholar]
  13. Ephraim RKD, Duah E, Cybulski JS, Prakash M, D'Ambrosio MV, Fletcher DA, Keiser J, Andrews JR, Bogoch II, 2015. Diagnosis of Schistosoma haematobium infection with a mobile phone-mounted foldscope and a reversed-lens CellScope in Ghana. Am J Trop Med Hyg 92: 12531256.[Crossref]
    [Google Scholar]
  14. WHO, 2009. Malaria Microscopy Quality Assurance Manual—Version 1. Geneva, Switzerland: World Health Organization.
    [Google Scholar]
  15. WHO, 2010. Basic Malaria Microscopy, Part I. Learner's Guide, 2nd edition. Geneva, Switzerland: World Health Organization.
    [Google Scholar]
  16. WHO Malaria Policy Advisory Committee and Secretariat, 2013. Malaria Policy Advisory Committee to the WHO: conclusions and recommendations of September 2013 meeting. Malar J 12: 456.[Crossref]
    [Google Scholar]
  17. Collier JA, Longmore JM, 1983. The reliability of the microscopic diagnosis of malaria in the field and in the laboratory. Ann Trop Med Parasitol 77: 113117.[Crossref]
    [Google Scholar]
  18. Longmore M, 1986. Community laboratory work and hand held microscopes. Trans R Soc Trop Med Hyg 80: 849850.[Crossref]
    [Google Scholar]
  19. Koydemir HC, Gorocs Z, Tseng D, Cortazar B, Feng S, Chan RYL, Burbano J, McLeod E, Ozcan A, 2015. Rapid imaging, detection and quantification of Giardia lamblia cysts using mobile-phone based fluorescent microscopy and machine learning. Lab Chip 15: 12841293.[Crossref]
    [Google Scholar]
  20. D'Ambrosio MV, Bakalar M, Bennuru S, Reber C, Skandarajah A, Nilsson L, Switz N, Kamgno J, Pion S, Boussinesq M, Nutman TB, Fletcher DA, 2015. Point-of-care quantification of blood-borne filarial parasites with a mobile phone microscope. Sci Transl Med 7: 286re4.[Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.16-0328
Loading
/content/journals/10.4269/ajtmh.16-0328
Loading

Data & Media loading...

  • Received : 26 Apr 2016
  • Accepted : 16 Jun 2016
  • Published online : 05 Oct 2016
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error