1921
Volume 95, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

is a gram-negative bacterium that causes melioidosis. In this study, we examined the sequence types (STs) of 60 clinical isolates from patients with melioidosis in Hainan, a tropical island in southern China. The 60 clinical isolates were resolved into 30 STs. Among the STs, ST562 was also reported in Australia, and ST90 was also reported in the United States, probably from a traveler who had previously visited Asia. In addition, six novel STs were found in this study, including ST1395, ST1396, ST1397, ST1398, ST1399, and ST1443, whereas the remaining STs were mostly shared with other southeast Asian regions. Phylogenetic analysis of 60 isolates conducted using the eBURST v3 software segregated the 30 STs into three groups and 18 singletons. Our study shows genetic diversity of 60 clinical isolates of in Hainan Island.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.16-0280
2016-10-05
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/14761645/95/4/760.html?itemId=/content/journals/10.4269/ajtmh.16-0280&mimeType=html&fmt=ahah

References

  1. Kaestli M, Mayo M, Harrington G, Ward L, Watt F, Hill JV, Cheng AC, Currie BJ, , 2009. Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia. PLoS Negl Trop Dis 3: e364.[Crossref] [Google Scholar]
  2. Baker A, Tahani D, Gardiner C, Bristow KL, Greenhill AR, Warner J, , 2011. Groundwater seeps facilitate exposure to Burkholderia pseudomallei . Appl Environ Microbiol 77: 72437246.[Crossref] [Google Scholar]
  3. Rattanavong S, Wuthiekanun V, Langla S, Amornchai P, Sirisouk J, Phetsouvanh R, Moore CE, Peacock SJ, Buisson Y, Newton PN, , 2011. Randomized soil survey of the distribution of Burkholderia pseudomallei in rice fields in Laos. Appl Environ Microbiol 77: 532536.[Crossref] [Google Scholar]
  4. Limmathurotsakul D, Wuthiekanun V, Amornchai P, Wongsuwan G, Day NP, Peacock SJ, , 2012. Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil. Appl Environ Microbiol 78: 876877.[Crossref] [Google Scholar]
  5. Vongphayloth K, Rattanavong S, Moore CE, Phetsouvanh R, Wuthiekanun V, Sengdouangphachanh A, Phouminh P, Newton PN, Buisson Y, , 2012. Burkholderia pseudomallei detection in surface water in southern Laos using Moore's swabs. Am J Trop Med Hyg 86: 872877.[Crossref] [Google Scholar]
  6. Currie BJ, Jacups SP, , 2003. Intensity of rainfall and severity of melioidosis, Australia. Emerg Infect Dis 9: 15381542.[Crossref] [Google Scholar]
  7. Su HP, Chan TC, Chang CC, , 2011. Typhoon-related leptospirosis and melioidosis, Taiwan, 2009. Emerg Infect Dis 17: 13221324.[Crossref] [Google Scholar]
  8. Parameswaran U, Baird RW, Ward LM, Currie BJ, , 2012. Melioidosis at Royal Darwin Hospital in the big 2009–2010 wet season: comparison with the preceding 20 years. Med J Aust 196: 345348.[Crossref] [Google Scholar]
  9. Liu X, Pang L, Sim SH, Goh KT, Ravikumar S, Win MS, Tan G, Cook AR, Fisher D, Chai LY, , 2015. Association of melioidosis incidence with rainfall and humidity, Singapore, 2003–2012. Emerg Infect Dis 21: 159162.[Crossref] [Google Scholar]
  10. Chen PS, Chen YS, Lin HH, Liu PJ, Ni WF, Hsueh PT, Liang SH, Chen C, Chen YL, , 2015. Airborne transmission of melioidosis to humans from environmental aerosols contaminated with B. pseudomallei . PLoS Negl Trop Dis 9: e0003834.[Crossref] [Google Scholar]
  11. Suputtamongkol Y, Hall AJ, Dance DA, Chaowagul W, Rajchanuvong A, Smith MD, White NJ, , 1994. The epidemiology of melioidosis in Ubon Ratchatani, northeast Thailand. Int J Epidemiol 23: 10821090.[Crossref] [Google Scholar]
  12. Hill AA, Mayo M, Kaestli M, Price EP, Richardson LJ, Godoy D, Spratt BG, Currie BJ, , 2013. Melioidosis as a consequence of sporting activity. Am J Trop Med Hyg 89: 365366.[Crossref] [Google Scholar]
  13. Doker TJ, Sharp TM, Rivera-Garcia B, Perez-Padilla J, Benoit TJ, Ellis EM, Elrod MG, Gee JE, Shieh WJ, Beesley CA, Ryff KR, Traxler RM, Galloway RL, Haberling DL, Waller LA, Shadomy SV, Bower WA, Hoffmaster AR, Walke HT, Blaney DD, , 2015. Contact investigation of melioidosis cases reveals regional endemicity in Puerto Rico. Clin Infect Dis 60: 243250.[Crossref] [Google Scholar]
  14. White NJ, , 2003. Melioidosis. Lancet 361: 17151722.[Crossref] [Google Scholar]
  15. Cheng AC, Currie BJ, , 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383416.[Crossref] [Google Scholar]
  16. Limmathurotsakul D, Golding N, Dance DAB, Messina JP, Pigott DM, Moyes CL, Rolim DB, Bertherat E, Day NPJ, Peacock SJ, Hay SI, , 2016. Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis. Nature Microbiol 1: 15.[Crossref] [Google Scholar]
  17. Godoy D, Randle G, Simpson AJ, Aanensen DM, Pitt TL, Kinoshita R, Spratt BG, , 2003. Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei . J Clin Microbiol 41: 20682079.[Crossref] [Google Scholar]
  18. Feil EJ, Li BC, Aanensen DM, Hanage WP, Spratt BG, , 2004. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186: 15181530.[Crossref] [Google Scholar]
  19. Cheng AC, Godoy D, Mayo M, Gal D, Spratt BG, Currie BJ, , 2004. Isolates of Burkholderia pseudomallei from northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation. J Clin Microbiol 42: 54775483.[Crossref] [Google Scholar]
  20. Cheng AC, Ward L, Godoy D, Norton R, Mayo M, Gal D, Spratt BG, Currie BJ, , 2008. Genetic diversity of Burkholderia pseudomallei isolates in Australia. J Clin Microbiol 46: 249254.[Crossref] [Google Scholar]
  21. Currie BJ, Thomas AD, Godoy D, Dance DA, Cheng AC, Ward L, Mayo M, Pitt TL, Spratt BG, , 2007. Australian and Thai isolates of Burkholderia pseudomallei are distinct by multilocus sequence typing: revision of a case of mistaken identity. J Clin Microbiol 45: 38283829.[Crossref] [Google Scholar]
  22. De Smet B, Sarovich DS, Price EP, Mayo M, Theobald V, Kham C, Heng S, Thong P, Holden MTG, Parkhill J, Peacock SJ, Spratt BG, Jacobs JA, Vandamme P, Currie BJ, , 2015. Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy. J Clin Microbiol 53: 323326.[Crossref] [Google Scholar]
  23. Price EP, Sarovich DS, Smith EJ, MacHunter B, Harrington G, Theobald V, Hall CM, Hornstra HM, McRobb E, Podin Y, Mayo M, Sahl JW, Wagner DM, Keim P, Kaestli M, Currie BJ, , 2016. Unprecedented melioidosis cases in northern Australia caused by an Asian Burkholderia pseudomallei strain identified by using large-scale comparative genomics. Appl Environ Microbiol 82: 954963.[Crossref] [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.16-0280
Loading
/content/journals/10.4269/ajtmh.16-0280
Loading

Data & Media loading...

  • Received : 11 Apr 2016
  • Accepted : 27 May 2016
  • Published online : 05 Oct 2016

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error