1921
Volume 95, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

In the United States, infection with has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione -transferase (GST)–FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect total IgG and IgG antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG, the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis.

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/content/journals/10.4269/ajtmh.16-0253
2016-10-05
2017-09-24
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  • Received : 01 Apr 2016
  • Accepted : 05 Jul 2016

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