Volume 95, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Visceral leishmaniasis (VL), or kala-azar, is mainly caused by two closely related species, and . is responsible for zoonotic VL, with dogs as the main reservoir host in the Mediterranean, the Middle East, Asia, and South America. In the Indian subcontinent, VL is caused by and is considered anthroponotic, although the only known vector, the sand fly, is zoophilic in nature. The role of domestic and stray dogs in VL transmission is still unclear in this area. We screened 50 stray dogs from VL-endemic areas of Bangladesh for serological and molecular evidence of infection. We detected anti- antibodies in six (12%) dog serum samples using rK39 immunochromatographic tests. We observed kinetoplast DNA in 10 (20%) buffy coat DNA samples by real-time polymerase chain reaction (PCR), five of which were positive based on internal transcribed spacer 1-PCR. A sequencing analysis of the amplified products confirmed that the parasitic DNA was derived from . Our findings support the hypothesis that stray dogs are an animal reservoir for in this endemic region. Further studies are required to determine the precise role of dogs in the epidemiology of VL in Bangladesh.


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  • Received : 26 Feb 2016
  • Accepted : 07 Jun 2016
  • Published online : 05 Oct 2016

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