1921
Volume 94, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity of in Mali. Genetic diversity was assessed via multiple loci variable number of tandem repeats analysis (MLVA). We performed DNA extraction from 104 positive and 30 negative used mRDTs that had been stored at ambient temperature for up to 14 months. Extracted DNA was analyzed via quantitative polymerase chain reaction (qPCR), and MLVA genotyping was then assessed on positive qPCR samples. Eighty-three of the positive mRDTs (83/104, 79.8%) and none of the negative mRDTs were confirmed positive via qPCR. We achieved complete genotyping of 90.4% (75/83) of the qPCR-positive samples. Genotyping revealed high genetic diversity among populations in Mali and an absence of population clustering. We show that mRDTs are useful to monitor genetic diversity and thereby can provide essential data to guide malaria control programs.

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2016-06-01
2017-11-20
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  • Received : 18 Nov 2015
  • Accepted : 15 Feb 2016

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