Volume 95, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A precise identification of species involved in human infections has epidemiological and clinical importance. Herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing species. After calmodulin amplification, the enzyme III yielded a clear distinction between reference strains of , , , , and the rest of the reference species analyzed. The closely related species: , , and , are separated in a subsequent digestion step with different restriction enzymes. We have developed a more accessible molecular protocol for identification/typing based on the exploitation of part of the calmodulin gene. This methodology has the potential to become an additional tool for species characterization and taxonomy.


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  • Received : 28 Sep 2015
  • Accepted : 15 May 2016
  • Published online : 03 Aug 2016

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