1921
Volume 94, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

A standardized test for the serodiagnosis of cystic echinococcosis (CE) remains an important challenge because of the problems in specificity and sensitivity of the available commercial kits and lack of proper evaluation of antigen. Using appropriate sources of antigenic material is crucial in improvement of the serological methods such as enzyme-linked immunosorbent assay (ELISA). This study was conducted to evaluate the performance of protein named protoscolex calcium binding protein EPC1 for the detection of antibodies in sera from patients with CE. Expressed and purified recombinant protein EPC1 (rEPC1) was used as antigen in ELISA method. Characterization of the rEPC1 antigen was evaluated using the serum of 25 patients with both surgical and imaging confirmed CE and 25 healthy donors as negative controls. Also, a panel of sera including chronic toxoplasmosis (IgG positive), strongyloidosis, fascioliasis, toxocariasis, and kala azar were used and patients with related parasites were confirmed by medical laboratories or clinically by research centers using microscopy or specific ELISA. rEPC1 showed relatively promising performance in total IgG ELISA for the detection of antibodies in sera from the negative controls, and the cut off value 0.4 units of optical density at 490 nm was calculated for ELISA. In this study, sensitivity of 100%, specificity of 93.7, positive predictive value of 92.6%, and negative predictive value of 100% were calculated for rEPC1. On the other hand, commercial ELISA kit based on the native antigen B of had sensitivity of 96.2% and specificity of 96.8%. No significant difference was found for sensitivity or specificity between the rEPC1 and commercial kit. However, rEPC1 may be a valuable antigen for diagnosis of human CE.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.15-0680
2016-06-01
2018-12-18
Loading full text...

Full text loading...

/deliver/fulltext/14761645/94/6/1313.html?itemId=/content/journals/10.4269/ajtmh.15-0680&mimeType=html&fmt=ahah

References

  1. WHO Eckert J, Gemmel MA, Meslin FX, Pawlowski ZS, , 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals, a Public Health Problem of Global Concern. , eds. Geneva, Switzerland: World Health Organization. [Google Scholar]
  2. Eslami A, Hosseini SH, , 1998. Echinococcus granulosus infection of farm dogs of Iran. Parasitol Res 84: 205207.[Crossref] [Google Scholar]
  3. Rokni MB, , 2009. Echinococcosis/hydatidosis in Iran. Iran J Parasitol 4: 116. [Google Scholar]
  4. Umhang G, Richomme C, Boucher JM, Hormaz V, Boue F, , 2013. Prevalence survey and first molecular characterization of Echinococcus granulosus in France. Parasitol Res 112: 18091812.[Crossref] [Google Scholar]
  5. Fasihi Harandi M, Budke CM, Rostami S, , 2012. The monetary burden of cystic echinococcosis in Iran. PLoS Negl Trop Dis 6: e1915.[Crossref] [Google Scholar]
  6. Torgerson PR, Deplazes P, , 2009. Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends Parasitol 25: 164170.[Crossref] [Google Scholar]
  7. Carmena D, Benito A, Eraso E, , 2006. Antigens for the immunodiagnosis of Echinococcus granulosus infection: an update. Acta Trop 98: 7486.[Crossref] [Google Scholar]
  8. Kalantari E, Bandehpour M, Pazoki R, Taghipoor-Lailabadi N, Khazan H, Mosaffa N, Nazaripouya MR, Kazemi B, , 2010. Application of recombinant Echinococcus granulosus antigen B to ELISA kits for diagnosing hydatidosis. Parasitol Res 106: 847851.[Crossref] [Google Scholar]
  9. Barnes TS, Li J, Coleman GT, McManus DP, , 2008. Development and evaluation of immunoblot-based serodiagnostic tests for hydatid infection in macropodids. J Wildl Dis 44: 10361040.[Crossref] [Google Scholar]
  10. Hernandez-Gonzalez A, Muro A, Barrera I, Ramos G, Orduna A, Siles-Lucas M, , 2008. Usefulness of four different Echinococcus granulosus recombinant antigens for serodiagnosis of unilocular hydatid disease (UHD) and postsurgical follow-up of patients treated for UHD. Clin Vaccine Immunol 15: 147153.[Crossref] [Google Scholar]
  11. Li J, Zhang WB, Wilson M, Ito A, McManus DP, , 2003. A novel recombinant antigen for immunodiagnosis of human cystic echinococcosis. J Infect Dis 188: 19511960.[Crossref] [Google Scholar]
  12. Virginio VG, Hernandez A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB, , 2003. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exe Imonol 132: 309315.[Crossref] [Google Scholar]
  13. Etebar F, Jalousian F, Hosseini SH, Kordafshari S, Najafi A, , 2013. Immunoproteomics approach for EPC1 antigenic epitope prediction of G1 and G6 strains of Echinococcus granulosus . Parasitol Res 112: 31293135.[Crossref] [Google Scholar]
  14. Kordafshari S, Hosseini SH, Jalousian F, Rajabibazl M, Jones M, Etebar F, , 2015. Evaluation of dot immunogold filtration assay (DIGFA) by recombinant protein EPC1 for anti-Echinococcus granulosus IgG antibody. Iran J Parasitol 10: 3038. [Google Scholar]
  15. Ben Nouir N, Gianinazzi C, Gorcii M, Muller N, Nouri A, Babba H, Gottstein B, , 2009. Isolation and molecular characterization of recombinant Echinococcus granulousus P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients. Trans R Soc Trop Med Hyg 103: 355364.[Crossref] [Google Scholar]
  16. Chen X, Chen X, Lu X, Feng X, Wen H, , 2015. The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay. Parasite Immunol 37: 1015.[Crossref] [Google Scholar]
  17. Li J, Zhang WB, Donald P, McManus DP, , 2004. Recombinant antigens for immunodiagnosis of cystic echinococcosis. Biol Proc 6: 6777.[Crossref] [Google Scholar]
  18. Zhang W, McManus DP, , 2006. Recent advances in the immunology and diagnosis of echinococcosis. FEMS Immunol Med Microbiol 47: 2441.[Crossref] [Google Scholar]
  19. Abdi J, Kazemi B, Mohebali M, Bandehpour M, Rahimi MT, Rokni MB, , 2010a. Gene cloning, expression and serological evaluation of EgAgB12 kDa from Echinococcus granulosus . Ann Trop Med Parasitol 104: 399407.[Crossref] [Google Scholar]
  20. Ioppolo S, Notargiacomo S, Profumo E, Franchi C, Ortona E, Rigano R, Siracusano A, , 1996. Immunological responses to antigen B from Echinococcus granulosus cyst fluid in hydatid patients. Parasite Immunol 18: 571578.[Crossref] [Google Scholar]
  21. Grimm F, Maly FE, Lu J, Llano R, , 1998. Analysis of specific immunoglobulin G subclass antibodies for serological diagnosis of echinococcosis by a standard enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol 5: 613616. [Google Scholar]
  22. McVie A, Ersfeld K, Rogan MT, Craig PS, , 1997. Expression and immunological characterisation of Echinococcis granulosus recombinant antigen B for IgG4 subclass detection in human cystic echinococcosis. Acta Trop 67: 1935.[Crossref] [Google Scholar]
  23. Wen H, Craig PS, , 1994. Immunoglobulin G subclass responses in human cystic and alveolar echinococcosis. Am J Trop Med Hyg 51: 741748. [Google Scholar]
  24. Ortona E, Margutti P, Delunardo F, Riganò R, Profumo E, Buttari B, Teggi A, Siracusano A, , 2004. Recombinant antigens of Echinococcus granulosus recognized by IgE and IgG4 of sera from patients with cystic echinococcosis. Parasitologia 46: 435436. [Google Scholar]
  25. Hernández-González A, Santivañez S, García HH, Rodríguez S, Muño S, Ramos G, Orduña A, Siles-Lucas M, , 2012. Improved serodiagnosis of cystic echinococcosis using the new recombinant 2B2t antigen. PLoS Negl Trop Dis 6: e1714.[Crossref] [Google Scholar]
  26. Valiente-Gabioud AA, Veaute C, Perrig M, Galan-Romano FS, Sferco SJ, Marcipar IS, , 2011. Effect of repetitiveness on the immunogenicity and antigenicity of Trypanosoma cruzi FRA protein. Exp Parasitol 127: 672679.[Crossref] [Google Scholar]
  27. Abdi J, Kazemi B, Haniloo A, Mohebali M, Mahmoudi M, Rezaei S, Bandehpour M, Maghen L, Rokni MB, , 2010. Serological evaluation of EgAgB16 kDa, a recombinant antigen from Echinococcus granulosus for diagnosis of human hydatidosis. Iran J Parasitol 5: 110. [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.15-0680
Loading
/content/journals/10.4269/ajtmh.15-0680
Loading

Data & Media loading...

  • Received : 17 Sep 2015
  • Accepted : 07 Mar 2016

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error