1921
Volume 95, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. and have been reported in Latin America, including the Amazon region. A morphological and molecular microfilariae study was performed in Pauini (Brazil). Blood samples were collected from 40 individuals, and were analyzed by Giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm protein gene. By microscopy, 14 of 40 were positive: 11 as and three as –like infections. Both molecular methods detected 19 positive cases as , including those 14 individuals detected by microscopy, without detectable genetic differences among any of the 19 positive samples. Molecular techniques showed an improvement of mansonellosis diagnosis and may become an effective tool to evaluate the present status of and in Latin America.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.15-0654
2016-09-07
2017-11-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/95/3/629.html?itemId=/content/journals/10.4269/ajtmh.15-0654&mimeType=html&fmt=ahah

References

  1. Walther M, Muller R, , 2003. Diagnosis of human filariases (except onchocerciasis). Adv Parasitol 53: 149193.[Crossref]
  2. Rosenblatt JE, , 2009. Laboratory diagnosis of infections due to blood and tissue parasites. Clin Infect Dis 49: 11031108.[Crossref]
  3. Beaver PC, Neel JV, Orihel TC, , 1976. Dipetalonema perstans and Mansonella ozzardi in Indians of southern Venezuela. Am J Trop Med Hyg 25: 263265.
  4. Gomez J, Guerrero R, , 2000. Environmental factors and the distribution of mansonelliases in southern Venezuela. Parasite 7: 7176.[Crossref]
  5. Marinkelle CJ, German E, , 1970. Mansonelliasis in the Comisaria del Vaupes of Colombia. Trop Geogr Med 22: 101111.
  6. Kozek WJ, Palma G, Henao A, Garcia H, Hoyos M, , 1983. Filariasis in Colombia: prevalence and distribution of Mansonella ozzardi and Mansonella (= Dipetalonema) perstans infections in the Comisaria del Guainia. Am J Trop Med Hyg 32: 379384.
  7. Orihel TC, , 1967. Infections with Dipetalonema perstans and Mansonella ozzardi in the aboriginal Indians of Guyana. Am J Trop Med Hyg 16: 628635.
  8. Adami YL, Moraes MA, Lanfredi RM, Maia-Herzog M, , 2008. An atypical microfilaria in blood samples from inhabitants of Brazilian Amazon. Parasitol Res 104: 9599.[Crossref]
  9. Marcos LA, Arrospide N, Recuenco S, Cabezas C, Weil GJ, Fischer PU, , 2012. Genetic characterization of atypical Mansonella (Mansonella) ozzardi microfilariae in human blood samples from northeastern Peru. Am J Trop Med Hyg 87: 491494.[Crossref]
  10. Godoy GA, Orihel TC, Volcan GS, , 1980. Microfilaria bolivarensis: a new species of filaria from man in Venezuela. Am J Trop Med Hyg 29: 545547.
  11. Eberhard ML, Lammie PJ, , 1991. Laboratory diagnosis of filariasis. Clin Lab Med 11: 9771010.
  12. Tang TH, Lopez-Velez R, Lanza M, Shelley AJ, Rubio JM, Luz SL, , 2010. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon region. Mem Inst Oswaldo Cruz 105: 823828.[Crossref]
  13. Hojas RM, Post RJ, , 2000. Regional genetic variation in the major sperm protein genes of Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea). Int J Parasitol 30: 14591465.[Crossref]
  14. Thompson JD, Higgins DG, Gibson TJ, , 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 46734680.[Crossref]
  15. Van de Peer Y, De Wachter R, , 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10: 569570.
  16. Luz SL, Crainey JL, Shelley AJ, Rubio M, , 2014. Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus. Mem Inst Oswaldo Cruz 109: 506508.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.15-0654
Loading
/content/journals/10.4269/ajtmh.15-0654
Loading

Data & Media loading...

  • Received : 09 Sep 2015
  • Accepted : 16 May 2016

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error