1921
Volume 94, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

On the shores of Lake Chad, schistosomiasis among mobile pastoralists was investigated in a field laboratory. Point-of-care circulating cathodic antigen (POC-CCA) cassette test, reagent strip, and filtration were conducted on urine samples. Fresh stool samples were subjected to the Kato-Katz technique, and fixed samples were examined with an ether-concentration method at a reference laboratory. POC-CCA urine cassette tests revealed a prevalence of 6.9%, compared with only 0.5% by stool microscopy. Three pregnant women with otherwise negative urine and stool testing had positive POC-CCA. This observation raises concern of cross-reactivity in pregnancy. Hence, two pregnant women in Switzerland with no history of schistosomiasis were subjected to POC-CCA and one tested positive. Our data suggest that POC-CCA can be performed under extreme Sahelian conditions (e.g., temperatures > 40°C), and it is more sensitive than stool microscopy for diagnosis. However, potential cross-reactivity in pregnancy needs further investigation.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.15-0577
2016-02-03
2017-09-23
Loading full text...

Full text loading...

/deliver/fulltext/14761645/94/2/361.html?itemId=/content/journals/10.4269/ajtmh.15-0577&mimeType=html&fmt=ahah

References

  1. Hotez PJ, Alvarado M, Basáñez MG, Bolliger I, Bourne R, Boussinesq M, Brooker SJ, Brown AS, Buckle G, Budke CM, Carabin H, Coffeng LE, Fèvre EM, Fürst T, Halasa YA, Jasrasaria R, Johns NE, Keiser J, King CH, Lozano R, Murdoch ME, O'Hanlon S, Pion SDS, Pullan RL, Ramaiah KD, Roberts T, Shepard DS, Smith JL, Stolk WA, Undurraga EA, Utzinger J, Wang M, Murray CJL, Naghavi M, , 2014. The Global Burden of Disease study 2010: interpretation and implications for the neglected tropical diseases. PLoS Negl Trop Dis 8: e2865.[Crossref]
  2. WHO, 2013. Schistosomiasis: Progress Report 2001–2011 and Strategic Plan 2012–2020. Geneva: World Health Organization.
  3. Colley DG, Bustinduy AL, Secor WE, King CH, , 2014. Human schistosomiasis. Lancet 383: 22532264.[Crossref]
  4. Utzinger J, Becker SL, van Lieshout L, van Dam GJ, Knopp S, , 2015. New diagnostic tools in schistosomiasis. Clin Microbiol Infect 21: 529542.[Crossref]
  5. van Dam GJ, Wichers JH, Ferreira TM, Ghati D, van Amerongen A, Deelder AM, , 2004. Diagnosis of schistosomiasis by reagent strip test for detection of circulating cathodic antigen. J Clin Microbiol 42: 54585461.[Crossref]
  6. Colley DG, Binder S, Campbell C, King CH, Tchuem Tchuenté LA, N'Goran EK, Erko B, Karanja DM, Kabatereine NB, van Lieshout L, Rathbun S, , 2013. A five-country evaluation of a point-of-care circulating cathodic antigen urine assay for the prevalence of Schistosoma mansoni . Am J Trop Med Hyg 88: 426432.[Crossref]
  7. Becker SL, Marti H, Zimmermann S, Vidacek D, Herrmann M, Utzinger J, Schnabel PA, Bohle RM, , 2015. Application in Europe of a urine-based rapid diagnostic test for confirmation of Schistosoma mansoni infection in migrants from endemic areas. Euro Surveill 20: 21151.[Crossref]
  8. Becquet R, Saout J, Pascal JM, , 1970. La bilharziose intestinale à Schistosoma intercalatum en Republique du Tchad (à propos de deux observations). Bull Soc Pathol Exot 63: 343350.
  9. Delpy P, Sirol J, Troncy J, Bono O, , 1972. Problèmes diagnostiques posés à Fort-Lamy (Tchad) par la présence d'oeufs de schistosome à éperon terminal dans les selles de malades indemnes de bilharziose urinaire. Bull Soc Pathol Exot 65: 417430.
  10. Massenet D, Abakar D, Karifene R, , 1995. Prévalence de la bilharziose urinaire en milieu scolaire à N'Djamena (Tchad). Bull Soc Pathol Exot 88: 3537.
  11. Hamit MA, Tidjani MT, Bilong Bilong CF, , 2008. Recent data on the prevalence of intestinal parasites in N'Djamena, Chad Republic. Afr J Environ Sci Technol 2: 407411.
  12. Massenet D, Gamougam K, Djerane L, Toukour A, , 2012. Cinquante années de diagnostics coprologiques parasitaires au Tchad et au Nord-Cameroun (1963–2009). Med Sante Trop 22: 330331.
  13. Moser W, Greter H, Schindler C, Allan F, Ngandolo BNR, Moto DD, Utzinger J, Zinsstag J, , 2014. The spatial and seasonal distribution of Bulinus truncatus, Bulinus forskalii and Biomphalaria pfeifferi, the intermediate host snails of schistosomiasis, in N'Djamena, Chad. Geospat Health 9: 109118.[Crossref]
  14. WHO, 2002. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. WHO Tech Rep Ser 921: 157.
  15. Savioli L, Hatz C, Dixon H, Kisumku UM, Mott KE, , 1990. Control of morbidity due to Schistosoma haematobium on Pemba Island: egg excretion and hematuria as indicators of infection. Am J Trop Med Hyg 43: 289295.
  16. Katz N, Chaves A, Pellegrino J, , 1972. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop São Paulo 14: 397400.
  17. Utzinger J, Botero-Kleiven S, Castelli F, Chiodini PL, Edwards H, Köhler N, Gulletta M, Lebbad M, Manser M, Matthys B, N'Goran EK, Tannich E, Vounatsou P, Marti H, , 2010. Microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among European reference laboratories. Clin Microbiol Infect 16: 267273.[Crossref]
  18. Coulibaly JT, N'Gbesso YK, Knopp S, N'Guessan NA, Silué KD, van Dam GJ, N'Goran EK, Utzinger J, , 2013. Accuracy of urine circulating cathodic antigen test for the diagnosis of Schistosoma mansoni in preschool-aged children before and after treatment. PLoS Negl Trop Dis 7: e2109.[Crossref]
  19. Stothard JR, Kabatereine NB, Tukahebwa EM, Kazibwe F, Rollinson D, Mathieson W, Webster JP, Fenwick A, , 2006. Use of circulating cathodic antigen (CCA) dipsticks for detection of intestinal and urinary schistosomiasis. Acta Trop 97: 219228.[Crossref]
  20. Legesse M, Erko B, , 2007. Field-based evaluation of a reagent strip test for diagnosis of Schistosoma mansoni by detecting circulating cathodic antigen in urine before and after chemotherapy. Trans R Soc Trop Med Hyg 101: 668673.[Crossref]
  21. Ayele B, Erko B, Legesse M, Hailu A, Medhin G, , 2008. Evaluation of circulating cathodic antigen (CCA) strip for diagnosis of urinary schistosomiasis in Hassoba school children, Afar, Ethiopia. Parasite 15: 6975.[Crossref]
  22. Midzi N, Butterworth AE, Mduluza T, Munyati S, Deelder AM, van Dam GJ, , 2009. Use of circulating cathodic antigen strips for the diagnosis of urinary schistosomiasis. Trans R Soc Trop Med Hyg 103: 4551.[Crossref]
  23. Coulibaly JT, Knopp S, N'Guessan NA, Silué KD, Fürst T, Lohourignon LK, Brou JK, N'Gbesso YK, Vounatsou P, N'Goran EK, Utzinger J, , 2011. Accuracy of urine circulating cathodic antigen (CCA) test for Schistosoma mansoni diagnosis in different settings of Côte d'Ivoire. PLoS Negl Trop Dis 5: e1384.[Crossref]
  24. Tchuem Tchuenté LA, Kuete Fouodo CJ, Kamwa Ngassam RI, Sumo L, Dongmo Noumedem C, Kenfack CM, Gipwe NF, Nana ED, Stothard JR, Rollinson D, , 2012. Evaluation of circulating cathodic antigen (CCA) urine-tests for diagnosis of Schistosoma mansoni infection in Cameroon. PLoS Negl Trop Dis 6: e1758.[Crossref]
  25. Adriko M, Standley CJ, Tinkitina B, Tukahebwa EM, Fenwick A, Fleming FM, Sousa-Figueiredo JC, Stothard JR, Kabatereine NB, , 2014. Evaluation of circulating cathodic antigen (CCA) urine-cassette assay as a survey tool for Schistosoma mansoni in different transmission settings within Bugiri district, Uganda. Acta Trop 136: 5057.[Crossref]
  26. Foo KT, Blackstock AJ, Ochola EA, Matete DO, Mwinzi PN, Montgomery SP, Karanja DM, Secor WE, , 2015. Evaluation of point-of-contact circulating cathodic antigen assays for the detection of Schistosoma mansoni infection in low-, moderate-, and high-prevalence schools in western Kenya. Am J Trop Med Hyg 92: 12271232.[Crossref]
  27. Mwinzi PN, Kittur N, Ochola E, Cooper PJ, Campbell CH, Jr King CH, Colley DG, , 2015. Additional evaluation of the point-of-contact circulating cathodic antigen assay for Schistosoma mansoni infection. Front Public Health 3: 48.[Crossref]
  28. Utzinger J, Booth M, N'Goran EK, Müller I, Tanner M, Lengeler C, , 2001. Relative contribution of day-to-day and intra-specimen variation in faecal egg counts of Schistosoma mansoni before and after treatment with praziquantel. Parasitology 122: 537544.[Crossref]
  29. Utzinger J, Keiser J, , 2004. Schistosomiasis and soil-transmitted helminthiasis: common drugs for treatment and control. Expert Opin Pharmacother 5: 263285.[Crossref]
  30. Allen HE, Crompton DWT, de Silva N, LoVerde PT, Olds GR, , 2002. New policies for using anthelmintics in high risk groups. Trends Parasitol 18: 381382.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.15-0577
Loading
/content/journals/10.4269/ajtmh.15-0577
Loading

Data & Media loading...

  • Received : 09 Aug 2015
  • Accepted : 25 Sep 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error