Volume 94, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The reservoir of causing Buruli ulcer (BU) remains unknown. Here, sterilized watery soil was mixed with 2 × 10 colony-forming units (CFU)/g of Agy99 or ATCC 33728 and incubated in a microaerophilic atmosphere in the presence of negative controls. Both strains survived in soil for 4 months with a final inoculum of 300–440 CFU/g. Further, three groups of five mice with and without footpad scarification were exposed to control soil or -inoculated soil. Although no specific clinical and histopathological lesions were observed in control animals, red spots observed on 8/20 scarified feet in 8/10 challenged mice yielded inflammatory infiltrates and positive real-time polymerase chain reaction detection of DNA in five mice. BU can be acquired as an inoculation infection with watery soil as a transient source of infection. These experimental observations warrant additional field observations.


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  • Received : 07 Aug 2015
  • Accepted : 30 Sep 2015
  • Published online : 06 Jan 2016

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