1921
Volume 94, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

Molecular approaches offer a means of testing archived samples stored as dried blood spots in settings where standard blood cultures are not possible. Peripheral blood films are one suggested source of material, although the sensitivity of this approach has not been well defined. Thin blood smears and dried blood spots from a severe pediatric malaria study were assessed using specific polymerase chain reaction (PCR) primers to detect non-typhoidal (NTS; gene), (), and (18S rRNA). Of 16 cases of NTS and confirmed on blood culture, none were positive by PCR using DNA extracts from blood films or dried blood spots. In contrast, four of 36 dried blood spots and two of 178 plasma samples were PCR positive for , despite negative bacterial blood cultures, suggesting false positives. Quantitative assessment revealed that the effective concentration of DNA in blood films was three log orders of magnitude lower than for dried blood spots. The gene could not be amplified from blood films. These findings question the value of blood PCR-based approaches for detection of NTS and , and show that stored blood films are an inefficient method of studying .

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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2016-02-03
2017-09-20
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  • Received : 20 Jul 2015
  • Accepted : 31 Oct 2015

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