1921
Volume 93, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

This study was performed to identify circulating proteins in patient serum, which may be useful as diagnostic markers. Depletion of highly abundant proteins from each pooled serum sample obtained from -infected patients and healthy individuals was performed using the Proteoseek Antibody-Based Albumin/IgG Removal Kit (Thermo Scientific, Rockford, IL). In analysis 1, the depleted serum was analyzed directly by NanoLC-MS/MS. In analysis 2, the depleted serum was separated by two-dimensional electrophoresis followed by western blot analysis. Subsequently, the selected band was analyzed by NanoLC-MS/MS. The result of analysis 1 revealed the presence of two mature erythrocyte surface antigen (MESA) proteins and chloroquine resistance transporter protein (PfCRT). In addition, analysis 2 revealed an antigenic 75-kDa band when the membrane was probed with purified IgG from the pooled serum obtained from -infected patients. MS/MS analysis of this protein band revealed fragments of MESA proteins. Thus, in this study, two different analyses revealed the presence of MESA protein in pooled serum from malaria patients; thus, this protein should be further investigated to determine its usefulness as a diagnostic marker.

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2015-12-09
2017-09-24
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References

  1. World Health Organization, 2013. World Malaria Report. Available at: http://www.who.int/malaria/publications/world_malaria_report_2013/report/en/. Accessed February 24, 2014.
  2. Gelhaus C, Fritsch J, Krause E, Leippe M, , 2005. Fractionation and identification of proteins by 2-DE and MS: towards a proteomic analysis of Plasmodium falciparum . Proteomics 5: 42134222.[Crossref]
  3. Parra ME, Evans CB, Taylor DW, , 1991. Identification of Plasmodium falciparum histidine-rich protein 2 in the plasma of humans with malaria. J Clin Microbiol 29: 16291634.
  4. Shevchenko A, Wilm M, Vorm O, Mann M, , 1996. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68: 850858.[Crossref]
  5. Ahmed N, Barker G, Oliva K, Garfin D, Talmadge K, Georgiou H, Quinn M, Rice G, , 2003. An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum. Proteomics 3: 19801987.[Crossref]
  6. Wiria AE, Prasetyani MA, Hamid F, Wammes LJ, Lell B, Ariawan I, Uh HW, Wibowo H, Djuardi Y, Wahyuni S, Sutanto I, May L, Luty AJ, Verweij JJ, Sartono E, Yazdanbakhsh M, Supali T, , 2010. Does treatment of intestinal helminth infections influence malaria? Background and methodology of a longitudinal study of clinical, parasitological and immunological parameters in Nangapanda, Flores, Indonesia (ImmunoSPIN Study). BMC Infect Dis 10: 77.[Crossref]
  7. MALDI SYNAPT G2 HDMS, 2010. System Overview and Maintenance Guide. 2010. Waters Rev B: 310, 3–13.
  8. Smit S, Stoychev S, Louw AI, Birkholtz LM, , 2010. Proteomic profiling of Plasmodium falciparum through improved, semiquantitative two-dimensional gel electrophoresis. J Proteome Res 9: 21702181.[Crossref]
  9. Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL, Witney AA, Wolters D, Wu Y, Gardner MJ, Holder AA, Sinden RE, Yates JR, Carucci DJ, , 2002. A proteomic view of the Plasmodium falciparum life cycle. Nature 419: 520526.[Crossref]
  10. Acharya P, Pallavi R, Chandran S, Chakravarti H, Middha S, Acharya J, Kochar S, Kochar D, Subudhi A, Boopathi AP, Garg S, Das A, Tatu U, , 2009. A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum and Plasmodium vivax . Proteomics Clin Appl 3: 13141325.[Crossref]
  11. Vignali M, Armour CD, Chen J, Morrison R, Castle JC, Biery MC, Bouzek H, Moon W, Babak T, Fried M, Raymond CK, Duffy PE, , 2011. NSR-seq transcriptional profiling enables identification of a gene signature of Plasmodium falciparum parasites infecting children. J Clin Invest 121: 11191129.[Crossref]
  12. Howard RJ, Lyon JA, Uni S, Saul AJ, Aley SB, Klotz F, Panton LJ, Sherwood JA, Marsh K, Aikawa M, , 1987. Transport of an Mr approximately 300,000 Plasmodium falciparum protein (Pf EMP 2) from the intraerythrocytic asexual parasite to the cytoplasmic face of the host cell membrane. J Cell Biol 104: 12691280.[Crossref]
  13. Kun JF, Waller KL, Coppel RL, , 1999. Plasmodium falciparum: structural and functional domains of the mature-parasite-infected erythrocyte surface antigen. Exp Parasitol 91: 258267.[Crossref]
  14. Maier AG, Cooke BM, Cowman AF, Tilley L, , 2009. Malaria parasite proteins that remodel the host erythrocyte. Nat Rev Microbiol 7: 341354.[Crossref]
  15. Waller KL, Nunomura W, An X, Cooke BM, Mohandas N, Coppel RL, , 2003. Mature parasite-infected erythrocyte surface antigen (MESA) of Plasmodium falciparum binds to the 30-kDa domain of protein 4.1 in malaria-infected red blood cells. Blood 102: 19111914.[Crossref]
  16. Magowan C, Coppel RL, Lau AO, Moronne MM, Tchernia G, Mohandas N, , 1995. Role of the Plasmodium falciparum mature-parasite-infected erythrocyte surface antigen (MESA/PfEMP-2) in malarial infection of erythrocytes. Blood 86: 31963204.
  17. Biswas S, Tomar D, Rao DN, , 2005. Investigation of the kinetics of histidine-rich protein 2 and of the antibody responses to this antigen, in a group of malaria patients from India. Ann Trop Med Parasitol 99: 553562.[Crossref]
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  • Received : 05 May 2015
  • Accepted : 06 Aug 2015

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