1921
Volume 96, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

The loop-mediated isothermal amplification (LAMP) assay with its advantages of cost effectiveness, rapidity, and simplicity, has evolved as a sensitive and specific method for the detection of African trypanosomes. Highly sensitive LAMP reactions specific for or that recognize but do not discriminate between , , , and have been developed. A sensitive LAMP assay targeting the 5.8S ribosomal RNA internal transcribed spacer 2 (5.8S-ITS2) gene is also available but this assay does not target binding sites that span the CCCA (CA) (557–560 bps) insertion site that further differentiates from . Here we describe 5.8S-ITS2-targeted LAMP assay that fit these criteria. The LAMP primer sets containing the -specific CA tetranucleotide at the start of the outer forward primer sequences showed high specificity and sensitivity down to at least 0.1 fg genomic DNA.

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References

  1. Blum JA, Neumayr AL, Hatz CF, , 2012. Human African trypanosomiasis in endemic populations and travellers. Eur J Clin Microbiol Infect Dis 31: 905913.[Crossref] [Google Scholar]
  2. Kennedy PG, , 2004. Human African trypanosomiasis of the CNS: current issues and challenges. J Clin Invest 113: 496504.[Crossref] [Google Scholar]
  3. Molyneux DH, Pentreath V, Doua F, , 1996. African Trypanosomiasis in Man. London, United Kingdom: WB Saunders. [Google Scholar]
  4. World Health Organization, 1998. Control and Surveillance of African Trypanosomiasis. Report of a WHO Expert Committee. Technical Report Series No. 881. Geneva, Switzerland: World Health Organization, 114. [Google Scholar]
  5. Bisser S, Lejon V, Preux PM, Bouteille B, Stanghellini A, Jauberteau MO, Buscher P, Dumas M, , 2002. Blood-cerebrospinal fluid barrier and intrathecal immunoglobulins compared to field diagnosis of central nervous system involvement in sleeping sickness. J Neurol Sci 193: 127135.[Crossref] [Google Scholar]
  6. Mori Y, Notomi T, , 2009. Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J Infect Chemother 15: 6269.[Crossref] [Google Scholar]
  7. Nagamine K, Hase T, Notomi T, , 2002. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16: 223229.[Crossref] [Google Scholar]
  8. Surabattula R, Vejandla MP, Mallepaddi PC, Faulstich K, Polavarapu R, , 2013. Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP). Exp Parasitol 134: 333340.[Crossref] [Google Scholar]
  9. Mori Y, Nagamine K, Tomita N, Notomi T, , 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289: 150154.[Crossref] [Google Scholar]
  10. Tomita N, Mori Y, Kanda H, Notomi T, , 2008. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3: 877882.[Crossref] [Google Scholar]
  11. Qiao YM, Guo YC, Zhang XE, Zhou YF, Zhang ZP, Wei HP, Yang RF, Wang DB, , 2007. Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores. Biotechnol Lett 29: 19391946.[Crossref] [Google Scholar]
  12. Wastling SL, Picozzi K, Kakembo AS, Welburn SC, , 2010. LAMP for human African trypanosomiasis: a comparative study of detection formats. PLoS Negl Trop Dis 4: e865.[Crossref] [Google Scholar]
  13. Kuboki N, Inoue N, Sakurai T, Di Cello F, Grab DJ, Suzuki H, Sugimoto C, Igarashi I, , 2003. Loop-mediated isothermal amplification for detection of African trypanosomes. J Clin Microbiol 41: 55175524.[Crossref] [Google Scholar]
  14. Njiru ZK, Mikosza AS, Matovu E, Enyaru JC, Ouma JO, Kibona SN, Thompson RC, Ndung'u JM, , 2008. African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol 38: 589599.[Crossref] [Google Scholar]
  15. Njiru ZK, Mikosza AS, Armstrong T, Enyaru JC, Ndung'u JM, Thompson AR, , 2008. Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense . PLoS Negl Trop Dis 2: e147.[Crossref] [Google Scholar]
  16. Mansfield JM, Paulnock DM, , 2005. Regulation of innate and acquired immunity in African trypanosomiasis. Parasite Immunol 27: 361371.[Crossref] [Google Scholar]
  17. Thekisoe OM, Kuboki N, Nambota A, Fujisaki K, Sugimoto C, Igarashi I, Yasuda J, Inoue N, , 2007. Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis. Acta Trop 102: 182189.[Crossref] [Google Scholar]
  18. Agbo EC, Majiwa PA, Claassen EJ, Roos MH, , 2001. Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization. Exp Parasitol 99: 123131.[Crossref] [Google Scholar]
  19. Grab DJ, Nikolskaia OV, Inoue N, Thekisoe MM, Morrison L, Gibson W, Dumler JS, , 2011. Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP). PLoS Negl Trop Dis 5: e1249.[Crossref] [Google Scholar]
  20. Aarthy R, Mani S, Velusami S, Sundarsingh S, Rajkumar T, , 2015. Role of circulating cell-free DNA in cancers. Mol Diagn Ther 6: 339350.[Crossref] [Google Scholar]
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  • Received : 13 Apr 2015
  • Accepted : 04 Mar 2016
  • Published online : 08 Feb 2017

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