1921
Volume 96, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The loop-mediated isothermal amplification (LAMP) assay with its advantages of cost effectiveness, rapidity, and simplicity, has evolved as a sensitive and specific method for the detection of African trypanosomes. Highly sensitive LAMP reactions specific for or that recognize but do not discriminate between , , , and have been developed. A sensitive LAMP assay targeting the 5.8S ribosomal RNA internal transcribed spacer 2 (5.8S-ITS2) gene is also available but this assay does not target binding sites that span the CCCA (CA) (557–560 bps) insertion site that further differentiates from . Here we describe 5.8S-ITS2-targeted LAMP assay that fit these criteria. The LAMP primer sets containing the -specific CA tetranucleotide at the start of the outer forward primer sequences showed high specificity and sensitivity down to at least 0.1 fg genomic DNA.

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/content/journals/10.4269/ajtmh.15-0288
2017-02-08
2017-09-23
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  • Received : 13 Apr 2015
  • Accepted : 04 Mar 2016

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