Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying . In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media—non-nutrient agar (NNA), culture on liquid growth media—peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.


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  1. Khan NA, , 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev 30: 564595.[Crossref] [Google Scholar]
  2. Visvesvara GS, Moura H, Schuster FL, , 2007. Pathogenic and opportunistic free‐living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea . FEMS Immunol Med Microbiol 50: 126.[Crossref] [Google Scholar]
  3. Baig AM, Zuberi H, Khan NA, , 2014. Recommendations for the management of Acanthamoeba keratitis. J Med Microbiol 63: 770771.[Crossref] [Google Scholar]
  4. Radford CF, Minassian DC, Dart JKG, , 2002. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risk factors. Br J Ophthalmol 86: 536542.[Crossref] [Google Scholar]
  5. Dart JKG, Saw VPJ, Kilvington S, , 2009. Acanthamoeba keratitis: diagnosis and treatment update 2009. Am J Ophthalmol 148: 487499.[Crossref] [Google Scholar]
  6. Sharma S, Garg P, Rao GN, , 2000. Patient characteristics, diagnosis, and treatment of non-contact lens related Acanthamoeba keratitis. Br J Ophthalmol 84: 11031108.[Crossref] [Google Scholar]
  7. Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R, , 2000. Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 118: 178183.[Crossref] [Google Scholar]
  8. Schroeder JM, Booton GC, Hay J, Niszl IA, Seal DV, Markus MB, Fuerst PA, Byers TJ, , 2001. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. J Clin Microbiol 39: 19031911.[Crossref] [Google Scholar]
  9. Thompson PP, Kowalski RP, Shanks RM, Gordon YJ, , 2008. Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis. J Clin Microbiol 46: 32323236.[Crossref] [Google Scholar]
  10. Borin S, Feldman I, Ken-Dror S, Briscoe D, , 2013. Rapid diagnosis of Acanthamoeba keratitis using non-nutrient agar with a lawn of E. coli . J Ophthalmic Inflamm Infect 3: 12.[Crossref] [Google Scholar]
  11. Pérez-Santonja JJ, Kilvington S, Hughes R, Tufail A, Matheson M, Dart JK, , 2003. Persistently culture positive Acanthamoeba keratitis: in vivo resistance and in vitro sensitivity. Ophthalmology 110: 15931600.[Crossref] [Google Scholar]
  12. Booton GC, Joslin CE, Shoff M, Tu EY, Kelly DJ, Fuerst PA, , 2009. Genotypic identification of Acanthamoeba sp. isolates associated with an outbreak of Acanthamoeba keratitis (AK). Cornea 28: 673.[Crossref] [Google Scholar]
  13. Graffi S, Peretz A, Jabaly H, Naftali M, , 2013. Acanthamoeba keratitis. Isr Med Assoc J 15: 182185. [Google Scholar]
  14. Yera H, Zamfir O, Bourcier T, Ancelle T, Batellier L, Dupouy-Camet J, Chaumeil C, , 2007. Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections. Eur J Clin Microbiol Infect Dis 26: 221224.[Crossref] [Google Scholar]
  15. Boggild AK, Martin DS, Lee TY, Yu B, Low DE, , 2009. Laboratory diagnosis of amoebic keratitis: comparison of four diagnostic methods for different types of clinical specimens. J Clin Microbiol 47: 13141318.[Crossref] [Google Scholar]
  16. Lorenzo-Morales J, Khan NA, Walochnik J, , 2015. An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22: 10.[Crossref] [Google Scholar]

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  • Received : 11 Mar 2015
  • Accepted : 07 Apr 2015
  • Published online : 05 Aug 2015

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