1921
Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of and . Diagnosis in humans is usually based on clinical symptoms and serology. Immunoglobulin G (IgG)-enzyme-linked immunosorbent assay kits using excretory–secretory (TES) larval antigens are commonly used for serodiagnosis. Differences in the antigens of the two species may influence the diagnostic sensitivity of the test. In this study, recombinant TES-120 (rTES-120) was cloned, expressed, and compared with its homolog in an IgG4-western blot. The diagnostic sensitivity and specificity of rTES-120 were 70% (33/47) and 100% (39/39), respectively. rTES-120 showed 57.4% sensitivity and 94.4% specificity. When the results of assays using rTES-120 of both species were considered, the diagnostic sensitivity was 76%. This study shows that using antigens from both species may improve the serodiagnosis of toxocariasis.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.15-0190
2015-08-05
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/14761645/93/2/319.html?itemId=/content/journals/10.4269/ajtmh.15-0190&mimeType=html&fmt=ahah

References

  1. Magnaval JF, Glickman LT, Dorchies P, Morassin B, , 2001. Highlights of human toxocariasis. Korean J Parasitol 39: 111.[Crossref] [Google Scholar]
  2. Smith H, Holland C, Taylor M, Magnaval JF, Schantz P, Maizels R, , 2009. How common is human toxocariasis? Towards standardizing our knowledge. Trends Parasitol 25: 182188.[Crossref] [Google Scholar]
  3. Fisher M, , 2003. Toxocara cati: an underestimated zoonotic agent. Trends Parasitol 19: 167170.[Crossref] [Google Scholar]
  4. Smith H, Noordin R, Holland CV, Smith HV, , 2006. Diagnostic limitations and future trends in the serodiagnosis of human toxocariasis. , eds. Toxocara: The Enigmatic Parasite. Oxfordshire, United Kingdom: CABI Publishing, 89112. [Google Scholar]
  5. Overgaauw PAM, Nederland V, , 1997. Aspects of Toxocara epidemiology: human toxocarosis. Crit Rev Microbiol 23: 215231.[Crossref] [Google Scholar]
  6. Jansen J, Knapen FV, , 1993. Toxocara eggs in public parks and sand-boxes in Utrecht. Tijdschr Diergeneeskd 118: 611614. [Google Scholar]
  7. Overgaauw PAM, Knapen FV, , 2008. Toxocarosis, an important zoonosis. European J Companion Animal Practice 18: 259266. [Google Scholar]
  8. Wilkins PP, , 2014. Immunodiagnosis of human toxocariasis and prospects for improved diagnostics. Curr Trop Med Rep 1: 4451.[Crossref] [Google Scholar]
  9. Xinou E, Lefkopoulos A, Gelagoti M, Drevelegas A, Diakou A, Milonas I, Dimitriadis AS, , 2003. CT and MR imaging findings in cerebral toxocaral disease. AJNR Am J Neuroradiol 24: 714718. [Google Scholar]
  10. Lee AC, Schantz PM, Kazacos KR, Montgomery SP, Bowman DD, , 2010. Epidemiologic and zoonotic aspects of ascarid infections in dogs and cats. Trends Parasitol 26: 155161.[Crossref] [Google Scholar]
  11. Yamasaki H, Araki K, Lim PK, Zasmy N, Mak JW, Taib R, Aoki T, , 2000. Development of a highly specific recombinant Toxocara canis second-stage larva excretory-secretory antigen for immunodiagnosis of human toxocariasis. J Clin Microbiol 38: 14091413. [Google Scholar]
  12. Zibaei M, Sadjjadi SM, Sarkari B, , 2007. Prevalence of Toxocara cati and other intestinal helminths in stray cats in Shiraz, Iran. Trop Biomed 24: 3943. [Google Scholar]
  13. Alcântara-Neves NM, dos Santos AB, Mendonça LR, Figueiredo CA, Pontes-de-Carvalho L, , 2008. An improved method to obtain antigen-excreting Toxocara canis larvae. Exp Parasitol 119: 349351.[Crossref] [Google Scholar]
  14. Mohamad S, Azmi NC, Noordin R, , 2009. Development and evaluation of a sensitive and specific assay for diagnosis of human toxocariasis by use of three recombinant antigens (TES-26, TES-30USM, and TES-120). J Clin Microbiol 47: 17121717.[Crossref] [Google Scholar]
  15. Zibaei M, Sadjjadi SM, Jahadi-Hosseini SH, , 2014. Toxocara cati larvae in the eye of a child: a case report. Asian Pac J Trop Biomed 4: S53S55.[Crossref] [Google Scholar]
  16. Petithory JC, Chaumeil C, Liotet S, Rosseau M, Bisognani C, Lewis JW, Maizels RM, , 1993. Immunological studies on ocular larva migrans. , eds. Toxocara and Toxocariasis: Clinical, Epidemiological and Molecular Perspectives. London, United Kingdom: The British Society for Parasitology with the Institute of Biology, 181189. [Google Scholar]
  17. Sakai R, Kawashima H, Shibui H, Kamata K, Kambara C, Matsuoka H, , 1998. Toxocara cati-induced ocular toxocariasis. Arch Ophthalmol 116: 16861687. [Google Scholar]
  18. Shimokawa H, , 1982. Visceral larva migrans by Toxocara cati . Fukuoka Igaku Zasshi 73: 6469. [Google Scholar]
  19. Roldan WH, Espinoza YA, , 2009. Evaluation of an enzyme-linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis. Mem Inst Oswaldo Cruz 104: 411418.[Crossref] [Google Scholar]
  20. Maizels RM, , 2013. Toxocara canis: molecular basis of immune recognition and evasion. Vet Parasitol 193: 365374.[Crossref] [Google Scholar]
  21. Hotez PJ, Wilkins PP, , 2009. Toxocariasis: America's most common neglected infection of poverty and a helminthiasis of global importance? PLoS Negl Trop Dis 3: e400.[Crossref] [Google Scholar]
  22. Kennedy MW, Maizels RM, Meghji M, Young L, Qureshi F, Smith HV, , 1987. Species-specific and common epitopes on the secreted and surface antigens of Toxocara cati and Toxocara canis infective larvae. Parasite Immunol 9: 407420.[Crossref] [Google Scholar]
  23. Poulsen CS, Skov A, Yoshida A, Skallerup P, Maruyama H, Thamsborg SM, Nejsum P, , 2015. Differential serodiagnostics of Toxocara canis and T. cati—is it possible? Parasite Immunol. doi:10.1111/pim.12181. [Google Scholar]
  24. Speiser F, Gottstein B, , 1984. A collaborative study on larval excretory/secretory antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Trop 41: 361372. [Google Scholar]
  25. Fong MY, Lau YL, Init I, Jamaiah I, Anuar AK, Noordin R, , 2003. Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli . Southeast Asian J Trop Med Public Health 34: 723726. [Google Scholar]
  26. Fong MY, Lau YL, , 2004. Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris . Parasitol Res 92: 173176.[Crossref] [Google Scholar]
  27. Noordin R, Smith HV, Mohamad S, Maizels RM, Fong MY, , 2005. Comparison of IgG-ELISA and IgG4-ELISA for Toxocara serodiagnosis. Acta Trop 93: 5762.[Crossref] [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.15-0190
Loading
/content/journals/10.4269/ajtmh.15-0190
Loading

Data & Media loading...

  • Received : 11 Mar 2015
  • Accepted : 14 Apr 2015
  • Published online : 05 Aug 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error