1921
Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of and . Diagnosis in humans is usually based on clinical symptoms and serology. Immunoglobulin G (IgG)-enzyme-linked immunosorbent assay kits using excretory–secretory (TES) larval antigens are commonly used for serodiagnosis. Differences in the antigens of the two species may influence the diagnostic sensitivity of the test. In this study, recombinant TES-120 (rTES-120) was cloned, expressed, and compared with its homolog in an IgG4-western blot. The diagnostic sensitivity and specificity of rTES-120 were 70% (33/47) and 100% (39/39), respectively. rTES-120 showed 57.4% sensitivity and 94.4% specificity. When the results of assays using rTES-120 of both species were considered, the diagnostic sensitivity was 76%. This study shows that using antigens from both species may improve the serodiagnosis of toxocariasis.

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2015-08-05
2017-11-23
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  • Received : 11 Mar 2015
  • Accepted : 14 Apr 2015

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