Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for spp. Ten pathogenic isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin/clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, nitrofurantoin, penicillin, piperacillin/tazobactam, and tetracycline). All 10 isolates had no zone of growth inhibition for four antimicrobials (fosfomycin, nalidixic acid, rifampicin, and trimethoprim/sulfamethoxazole). Of the ten , seven had a growth inhibition zone of ≤ 21 mm for aztreonam, the zone diameter susceptibility break point for Enterobacteriaceae. This assay could find utility as a simple screening method during the epidemiological surveillance of antimicrobial resistance in spp.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Levett PN, 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref]
    [Google Scholar]
  2. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757771.[Crossref]
    [Google Scholar]
  3. World Health Organization, 2003. Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Available at: http://www.who.int/zoonoses/resources/Leptospirosis/en/.
    [Google Scholar]
  4. Wuthiekanun V, Amornchai P, Paris DH, Langla S, Thaipadunpanit J, Chierakul W, Smythe LD, White NJ, Day NP, Limmathurotsakul D, Peacock SJ, 2012. Rapid isolation and susceptibility testing of Leptospira spp. using a new solid medium (LVW agar). Antimicrob Agents Chemother 57: 297302.[Crossref]
    [Google Scholar]
  5. Wuthiekanun V, Amornchai P, Langla S, Oyuchua M, Day NP, Limmathurotsakul D, 2014. Maintenance of Leptospira species in Leptospira Vanaporn Wuthiekanun agar. J Clin Microbiol 52: 43504352.[Crossref]
    [Google Scholar]
  6. Thaipadungpanit J, Wuthiekanun V, Chantratita N, Yimsamran S, Amornchai P, Boonsilp S, Maneeboonyang W, Tharnpoophasiam P, Saiprom N, Mahakunkijcharoen Y, Day NP, Singhasivanon P, Peacock SJ, Limmathurotsakul D, 2013. Leptospira species in floodwater during the 2011 floods in the Bangkok Metropolitan Region, Thailand. Am J Trop Med Hyg 89: 794796.[Crossref]
    [Google Scholar]
  7. Murray CK, Hospenthal DR, 2004. Determination of susceptibilities of 26 Leptospira sp. serovars to 24 antimicrobial agents by a broth microdilution technique. Antimicrob Agents Chemother 48: 40024005.[Crossref]
    [Google Scholar]
  8. Chakraborty A, Miyahara S, Villanueva SY, Gloriani NG, Yoshida S, 2010. In vitro sensitivity and resistance of 46 Leptospira strains isolated from rats in the Philippines to 14 antimicrobial agents. Antimicrob Agents Chemother 54: 54035405.[Crossref]
    [Google Scholar]

Data & Media loading...

Supplementary PDF

  • Received : 05 Mar 2015
  • Accepted : 01 Apr 2015
  • Published online : 05 Aug 2015
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error