Volume 93, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Brucellosis remains a serious public health issue in developing countries, including China. On August 8, 2013, four cases of brucellosis from one extended family were reported at Shuyang County, Jiangsu Province, China. Active case finding was performed to identify the source and the risk factors of the infection and to prevent additional cases. Multiple-locus variable number tandem repeat analysis (MLVA) was used for molecular subtyping analysis. Six people from two extended families met the case definition for brucellosis infection; four were blood culture positive for biotype 3. Four additional family members were found seropositive by using a serological test. Isolates from the four patients were indistinguishable by MLVA profiling, displaying a unique type for Jiangsu Province. Field epidemiological data combined with MLVA genotyping supported a common source of the isolates from the different patients. We recommend stronger reinforcement measures for animal quarantine practices, enhanced cooperation with veterinary service organizations, and implementation of measures that strengthen public education on brucellosis to prevent further human outbreaks in Jiangsu Province.


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  1. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV, , 2006. The new global map of human brucellosis. Lancet Infect Dis 6: 9199.
  2. Pappas G, Akritidis N, Bosilkovski M, Tsianos E, , 2013. Brucellosis. N Engl J Med 352: 23252336.
  3. Chen JD, Ke CW, Deng X, Jiang S, Liang W, Ke BX, Li B, Tan H, Liu M, , 2013. Brucellosis in Guangdong Province, People's Republic of China, 2005–2010. Emerg Infect Dis 19: 817818.
  4. Man T, Wang D, Cui B, Wang Y, Ding F, Li TF, , 2009. Analysis on surveillance data of brucellosis in China, 2009 [in Chinese]. Dis Surveill 25: 944946.
  5. Mukherjee F, Jain J, Patel V, Nair M, , 2007. Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals. J Med Microbiol 56: 13091316.
  6. Bricker BJ, Halling SM, , 1994. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 32: 26602666.
  7. Araj GF, , 2010. Update on laboratory diagnosis of human brucellosis. Int J Antimicrob 36: S12S17.
  8. Le Flèche P, Jacques I, Grayon M, Al Dahouk S, Bouchon P, Denoeud F, Nöckler K, Neubauer H, Guilloteau LA, Vergnaud G, , 2006. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 6: 9.
  9. Whatmore AM, Shankster SJ, Perrett LL, Murphy TJ, Brew SD, Thirlwall RE, Cutler SJ, MacMillan AP, , 2006. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J Clin Microbiol 44: 19821993.
  10. Jiang H, Fan M, Chen J, Mi J, Yu R, Zhao H, Piao D, Ke C, Deng X, Tian G, Cui B, , 2011. MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates. BMC Microbiol 11: 256.
  11. Kılıç S, Ivanov IN, Durmaz R, Bayraktar MR, Ayaslioglu E, Uyanik MH, Aliskan H, Yasar E, Bayramoglu G, Arslantürk A, Vergnaud G, Kantardjiev TV, , 2011. Multiple-locus variable-number tandem-repeat analysis genotyping of human Brucella isolates from Turkey. J Clin Microbiol 49: 32763283.
  12. Plumb G, Olsen S, Buttke D, , 2013. Brucellosis: ‘One Health’ challenges and opportunities. Rev Sci Tech Oie 32: 271278.

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  • Received : 22 Feb 2015
  • Accepted : 01 Apr 2015

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