1921
Volume 93, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Loss of sample integrity during specimen transport can lead to false-negative diagnostic results. In an effort to improve upon the status quo, we used dengue as a model RNA virus to evaluate the stabilization of RNA and antibodies in three commercially available sample stabilization products: Whatman FTA Micro Cards (GE Healthcare Life Sciences, Pittsburgh, PA), DNAstāble Blood tubes (Biomātrica, San Diego, CA), and ViveST tubes (ViveBio, Alpharetta, GA). Both contrived and clinical dengue-positive specimens were stored on these products at ambient temperature or 37°C for up to 1 month. Antibody and viral RNA levels were measured by enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays, respectively, and compared with frozen unloaded controls. We observed reduced RNA and antibody levels between stabilized contrived samples and frozen controls at our earliest time point, and this was particularly pronounced for the FTA cards. However, despite some time and temperature dependent loss, a 94.6–97.3% agreement was observed between stabilized clinical specimens and their frozen controls for all products. Additional considerations such as cost, sample volume, matrix, and ease of use should inform any decision to incorporate sample stabilization products into a diagnostic testing workflow. We conclude that DNAstāble Blood and ViveST tubes are useful alternatives to traditional filter paper for ambient temperature shipment of clinical specimens for downstream molecular and serological testing.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.15-0110
2015-07-08
2017-09-20
Loading full text...

Full text loading...

/deliver/fulltext/14761645/93/1/46.html?itemId=/content/journals/10.4269/ajtmh.15-0110&mimeType=html&fmt=ahah

References

  1. Thorp HH, , 2000. The importance of being r: greater oxidative stability of RNA compared with DNA. Chem Biol 7: R33R36.[Crossref]
  2. CLSI, 2010. Quantitative Molecular Methods for Infectious Diseases; Approved Guideline—Second Edition. MM06-A2. Wayne, PA: Clinical and Laboratory Standards Institute.
  3. Olson WC, Smolkin ME, Farris EM, Fink RJ, Czarkowski AR, Fink JH, Chianese-Bullock KA, Slingluff CL, Jr, 2011. Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function. J Transl Med 9: 26.[Crossref]
  4. Catton M, Druce J, Papadakis G, Tran T, Birch C, , 2011. Reality check of laboratory service effectiveness during pandemic (H1N1) 2009, Victoria, Australia. Emerg Infect Dis 17: 963968.[Crossref]
  5. Barin F, Meyer L, Lancar W, Deveau C, Gharib M, Laporte A, Desenclos JC, Costagliola D, , 2005. Development and validation of an immunoassay for identification of recent human immunodeficiency virus type 1 infections and its use on dried serum spots. J Clin Microbiol 43: 44414447.[Crossref]
  6. Monleau M, Montavon C, Laurent C, Segondy M, Montes B, Delaporte E, Boillot F, Peeters M, , 2009. Evaluation of different RNA extraction methods and storage conditions of dried plasma or blood spots for human immunodeficiency virus type 1 RNA quantification and PCR amplification for drug resistance testing. J Clin Microbiol 47: 11071118.[Crossref]
  7. Abe K, Konomi N, , 1998. Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature. J Clin Microbiol 36: 30703072.
  8. Solmone M, Girardi E, Costa F, Pucillo L, Ippolito G, Capobianchi MR, , 2002. Simple and reliable method for detection and genotyping of hepatitis C virus RNA in dried blood spots stored at room temperature. J Clin Microbiol 40: 35123514.[Crossref]
  9. Fukunaga T, Rojanasuphot S, Pisuthipornkul S, Wungkorbkiat S, Thammanichanon A, , 1974. Seroepidemiologic study of arbovirus infections in the north-east and south of Thailand. Biken J 17: 169182.
  10. Top FT, Jr Gunakasem P, Chantrasri C, Supavadee J, , 1975. Serologic diagnosis of dengue haemorrhagic fever using filter paper discs and one dengue antigen. Southeast Asian J Trop Med Public Health 6: 1824.
  11. Prado I, Rosario D, Bernardo L, Alvarez M, Rodriguez R, Vazquez S, Guzman MG, , 2005. PCR detection of dengue virus using dried whole blood spotted on filter paper. J Virol Methods 125: 7581.[Crossref]
  12. Matheus S, Meynard JB, Lacoste V, Morvan J, Deparis X, , 2007. Use of capillary blood samples as a new approach for diagnosis of dengue virus infection. J Clin Microbiol 45: 887890.[Crossref]
  13. Balmaseda A, Saborio S, Tellez Y, Mercado JC, Perez L, Hammond SN, Rocha C, Kuan G, Harris E, , 2008. Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies. J Clin Virol 43: 287291.[Crossref]
  14. Anders KL, Nguyet NM, Quyen NT, Ngoc TV, Tram TV, Gan TT, Tung NT, Dung NT, Chau NV, Wills B, Simmons CP, , 2012. An evaluation of dried blood spots and oral swabs as alternative specimens for the diagnosis of dengue and screening for past dengue virus exposure. Am J Trop Med Hyg 87: 165170.[Crossref]
  15. Wan E, Akana M, Pons J, Chen J, Musone S, Kwok PY, Liao W, , 2010. Green technologies for room temperature nucleic acid storage. Curr Issues Mol Biol 12: 135142.
  16. Mei JV, Alexander JR, Adam BW, Hannon WH, , 2001. Use of filter paper for the collection and analysis of human whole blood specimens. J Nutr 131: 1631S1636S.
  17. Brandao CP, Marques BL, Marques VA, Villela-Nogueira CA, Do OK, de Paula MT, Lewis-Ximenez LL, Lampe E, Sa Ferreira JA, Villar LM, , 2013. Simultaneous detection of hepatitis C virus antigen and antibodies in dried blood spots. J Clin Virol 57: 98102.[Crossref]
  18. Villar LM, de Oliveira JC, Cruz HM, Yoshida CF, Lampe E, Lewis-Ximenez LL, , 2011. Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers. J Med Virol 83: 15221529.[Crossref]
  19. Demirev PA, , 2013. Dried blood spots: analysis and applications. Anal Chem 85: 779789.[Crossref]
  20. Howlett SE, Castillo HS, Gioeni LJ, Robertson JM, Donfack J, , 2014. Evaluation of DNAstable for DNA storage at ambient temperature. Forensic Sci Int Genet 8: 170178.[Crossref]
  21. Ivanova NV, Kuzmina ML, , 2013. Protocols for dry DNA storage and shipment at room temperature. Mol Ecol Resour 13: 890898.[Crossref]
  22. Zanoni M, Giron LB, Vilhena C, Sucupira MC, Lloyd RM, Jr Diaz RS, , 2012. Comparative effectiveness of dried-plasma hepatitis B virus viral load (VL) testing in three different VL commercial platforms using ViveST for sample collection. J Clin Microbiol 50: 145147.[Crossref]
  23. Zanoni M, Cortes R, Diaz RS, Sucupira MC, Ferreira D, Inocencio LA, Vilhena C, Loveday C, Lloyd RM, Jr Holodniy M, , 2010. Comparative effectiveness of dried plasma HIV-1 viral load testing in Brazil using ViveST for sample collection. J Clin Virol 49: 245248.[Crossref]
  24. Lloyd RM, Jr Burns DA, Huong JT, Mathis RL, Winters MA, Tanner M, De La Rosa A, Yen-Lieberman B, Armstrong W, Taege A, McClernon DR, Wetshtein JL, Friedrich BM, Ferguson MR, O'Brien W, Feorino PM, Holodniy M, , 2009. Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing. J Clin Microbiol 47: 14911496.[Crossref]
  25. Forshey BM, Guevara C, Laguna-Torres VA, Cespedes M, Vargas J, Gianella A, Vallejo E, Madrid C, Aguayo N, Gotuzzo E, Suarez V, Morales AM, Beingolea L, Reyes N, Perez J, Negrete M, Rocha C, Morrison AC, Russell KL, Blair PJ, Olson JG, Kochel TJ, NFSW Group, , 2010. Arboviral etiologies of acute febrile illnesses in western South America, 2000–2007. PLoS Negl Trop Dis 4: e787.[Crossref]
  26. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV, , 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545551.
  27. Johnson BW, Russell BJ, Lanciotti RS, , 2005. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol 43: 49774983.[Crossref]
  28. McAvin JC, Escamilla EM, Blow JA, Turell MJ, Quintana M, Bowles DE, Swaby JA, Barnes WJ, Huff WB, Lohman KL, Atchley DH, Hickman JR, Niemeyer DM, , 2005. Rapid identification of dengue virus by reverse transcription-polymerase chain reaction using field-deployable instrumentation. Mil Med 170: 10531059.[Crossref]
  29. Bland JM, Altman DG, , 1986. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1: 307310.[Crossref]
  30. Gilliland T, Jr Dauner AL, Pal S, Mitra S, Kochel T, Wu S-J, , 2013. Evaluation of Sample Integrity Using Commercially Available RNA-Stabilizing Blood Collection Tubes. Bethesda, MD: Uniformed Services University Research Day.
  31. Smit PW, Elliott I, Peeling RW, Mabey D, Newton PN, , 2014. An overview of the clinical use of filter paper in the diagnosis of tropical diseases. Am J Trop Med Hyg 90: 195210.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.15-0110
Loading
/content/journals/10.4269/ajtmh.15-0110
Loading

Data & Media loading...

Supplementary Data

Supplementary PDF

  • Received : 06 Feb 2015
  • Accepted : 24 Mar 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error