Volume 93, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Three human cases of H10N8 virus infections were initially reported in China in late 2013 and early 2014, two of which were fatal. This was the first time the H10N8 subtype has been detected in humans, and the pathogenicity of this virus remains under characterized. We first assessed its pathogenicity by infecting BALB/c mice with two H10N8 isolates, A/Jiangxi-Donghu/346-1/2013 and A/Chicken/Jiangxi/102/2013. The human isolate (H346-1) demonstrated stronger capability of replication and induced higher cytokine response in vivo than the chicken isolate (C102). In addition, H346-1 was fatal to mice, while all mice ( = 14) in C102-infected group survived during the infection course without weight loss. We hypothesized that the 627K mutation in the gene (PB2-K627) in H346-1 was associated with high pathogenicity in mice. Taken together, this study based on mouse model provides some insight into understanding the pathogenicity of the emerging viruses in mammals.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. García-Sastre A, Schmolke M, , 2014. Avian influenza A H10N8–a virus on the verge? Lancet 383: 676677.[Crossref] [Google Scholar]
  2. Kuiken T, Holmes EC, McCauley J, Rimmelzwaan GF, Williams CS, Grenfell BT, , 2006. Host species barriers to influenza virus infections. Science 312: 394397.[Crossref] [Google Scholar]
  3. Webster RG, Peiris M, Chen H, Guan Y, , 2006. H5N1 outbreaks and enzootic influenza. Emerg Infect Dis 12: 38.[Crossref] [Google Scholar]
  4. Wei SH, Yang JR, Wu HS, Chang MC, Lin JS, Lin CY, Liu YL, Lo YC, Yang CH, Chuang JH, Lin MC, Chung WC, Liao CH, Lee MS, Huang WT, Chen PJ, Liu MT, Chang FY, , 2013. Human infection with avian influenza A H6N1 virus: an epidemiological analysis. Lancet Respir Med 1: 771778.[Crossref] [Google Scholar]
  5. Peiris M, Yuen KY, Leung CW, Chan KH, Ip PL, Lai RW, Orr WK, Shortridge KF, , 1999. Human infection with influenza H9N2. Lancet 354: 916917.[Crossref] [Google Scholar]
  6. Belser JA, Bridges CB, Katz JM, Tumpey TM, , 2009. Past, present, and possible future human infection with influenza virus A subtype H7. Emerg Infect Dis 15: 859865.[Crossref] [Google Scholar]
  7. To KK, Tsang AK, Chan JF, Cheng VC, Chen H, Yuen KY, , 2014. Emergence in China of human disease due to avian influenza A (H10N8)–cause for concern? J Infect 68: 205215.[Crossref] [Google Scholar]
  8. Chen H, Yuan H, Gao R, Zhang J, Wang D, Xiong Y, Fan G, Yang F, Li X, Zhou J, Zou S, Yang L, Chen T, Dong L, Bo H, Zhao X, Zhang Y, Lan Y, Bai T, Dong J, Li Q, Wang S, Zhang Y, Li H, Gong T, Shi Y, Ni X, Li J, Zhou J, Fan J, Wu J, Zhou X, Hu M, Wan J, Yang W, Li D, Wu G, Feng Z, Gao GF, Wang Y, Jin Q, Liu M, Shu Y, , 2014. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet 383: 714721.[Crossref] [Google Scholar]
  9. Qi W, Zhou X, Shi W, Huang L, Xia W, Liu D, Li H, Chen S, Lei F, Cao L, Wu J, He F, Song W, Li Q, Li H, Liao M, Liu M, , 2014. Genesis of the novel human-infecting influenza A (H10N8) virus and potential genetic diversity of the virus in poultry, China. Euro Surveill 19: 20841.[Crossref] [Google Scholar]
  10. Reed LJ, Muench H, , 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493497. [Google Scholar]
  11. Hatta M, Gao P, Halfmann P, Kawaoka Y, , 2001. Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293: 18401842.[Crossref] [Google Scholar]
  12. Li X, Qi W, He J, Ning Z, Hu Y, Tian J, Jiao P, Xu C, Chen J, Richt J, Ma W, Liao M, , 2012. Molecular basis of efficient replication and pathogenicity of H9N2 avian influenza viruses in mice. PLoS One 7: e40118.[Crossref] [Google Scholar]
  13. Mok CK, Lee HH, Lestra M, Nicholls JM, Chan MC, Sia SF, Zhu H, Poon LL, Guan Y, Peiris JS, , 2014. Amino acid substitutions in polymerase basic protein 2 gene contributes to the pathogenicity of the novel A/H7N9 influenza virus in mammalian hosts. J Virol 88: 35683576.[Crossref] [Google Scholar]
  14. Arzey GG, Kirkland PD, Arzey KE, Frost M, Maywood P, Conaty S, Hurt AC, Deng YM, Iannello P, Barr I, Dwyer DE, Ratnamohan M, McPhie K, Selleck P, , 2012. Influenza virus A (H10N7) in chickens and poultry abattoir workers, Australia. Emerg Infect Dis 18: 814816.[Crossref] [Google Scholar]
  15. Beare AS, Webster RG, , 1991. Replication of avian influenza viruses in humans. Arch Virol 119: 3742.[Crossref] [Google Scholar]
  16. Zohari S, Neimanis A, Härkönen T, Moraeus C, Valarcher JF, , 2014. Avian influenza A (H10N7) virus involvement in mass mortality of harbour seals (Phoca vitulina) in Sweden, March through October 2014. Euro Surveill 19: 20967.[Crossref] [Google Scholar]
  17. Liu M, Li X, Yuan H, Zhou J, Wu J, Bo H, Xia W, Xiong Y, Yang L, Gao R, Guo J, Huang W, Zhang Y, Zhao X, Zou X, Chen T, Wang D, Li Q, Wang S, Chen S, Hu M, Ni X, Gong T, Shi Y, Li J, Zhou J, Cai J, Xiao Z, Zhang W, Sun J, Li D, Wu G, Feng Z, Wang Y, Chen H, Shu Y, , 2015. Genetic diversity of avian influenza A (H10N8) virus in live poultry markets and its association with human infections in China. Sci Rep 5: 7632.[Crossref] [Google Scholar]
  18. Ni X, He F, Hu M, Zhou X, Wang B, Feng C, Wu Y, Li Y, Tu J, Li H, Liu M, Chen H, Chen S, , 2015. Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A (H10N8) in Nanchang City, China. Microbes Infect 17: 4853.[Crossref] [Google Scholar]

Data & Media loading...

  • Received : 23 Jan 2015
  • Accepted : 27 Jul 2015
  • Published online : 09 Dec 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error