Volume 92, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



and species belong to a complex group of pathogens that cause diseases hampering development and socioeconomic improvements in the developing countries. Both pathogens are recognized as significant causes of diarrhea and nutritional disorders. However, further studies are needed to clarify the role of parasitic infections, especially asymptomatic infections in malnutrition and stunting. We developed a high-throughput multiplex quantitative polymerase chain reaction (qPCR) method for and spp. detection in stool samples. The sensitivity and specificity of the method were ensured by analyzing confirmed positive samples acquired from diagnostics laboratories and participating in an external quality control round. Its capability to detect asymptomatic and spp. infections was confirmed by analyzing stool samples collected from 44 asymptomatic 6-month-old infants living in an endemic region in Malawi. Of these, five samples were found to be positive for and two for spp. In conclusion, the developed method is suitable for large-scale studies evaluating the occurrence of and spp. in endemic regions and for clinical diagnostics of these infections.


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  • Received : 21 Jan 2015
  • Accepted : 27 Feb 2015
  • Published online : 03 Jun 2015

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