Volume 92, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Malaria was considered as the main cause of fever in Africa. However, with the roll back malaria initiative, the causes of fever in Africa may change. This study aimed to evaluate the prevalence of bacteria and spp. in febrile and afebrile (controls) children from Franceville, Gabon. About 793 blood samples from febrile children and 100 from controls were analyzed using polymerase chain reaction (PCR) coupled with sequencing. spp. was the microorganism most detected in febrile (74.5%, 591/793) and controls (13%, 13/100), < 0.0001. Its coinfection with bacteria was found only in febrile children ( = 0.0001). was the most prevalent bacterium in febrile children (2.8%, 22/793) and controls (3%, 3/100). Eight cases of spp. (including two serovar Paratyphi) and two of were found only among febrile children. spp. was found in 2 controls while was detected in 10 children (in 8 febriles and 2 afebriles). No DNA of other targeted microorganisms was detected. spp. remains prevalent while spp., , and were common bacteria in Gabon. Two fastidious bacteria, and spp., were found. Inclusion of controls should improve the understanding of the causes of fever in sub-Saharan Africa.


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  1. D'Acremont V, Lengeler C, Genton B, , 2010. Reduction in the proportion of fevers associated with Plasmodium falciparum parasitaemia in Africa: a systematic review. Malar J 9: 240.[Crossref] [Google Scholar]
  2. Bouyou-Akotet MK, Mawili-Mboumba DP, Kendjo E, Mabika-Mamfoumbi M, Ngoungou EB, Dzeing-Ella A, Pemba-Mihindou M, Ibinga E, Efame-Eya E, Planche T, Kremsner PG, Kombila M, , 2009. Evidence of decline of malaria in the general hospital of Libreville, Gabon from 2000 to 2008. Malar J 8: 300.[Crossref] [Google Scholar]
  3. Lekana-Douki JB, Pontarollo J, Zatra R, Toure-Ndouo FS, , 2011. Malaria in Gabon: results of a clinical and laboratory study at the Chinese-Gabonese Friendship Hospital of Franceville. Sante 21: 193198. [Google Scholar]
  4. Bouyou-Akotet MK, Mawili-Mboumba DP, Kendjo E, Eyang EA, Abdou RO, Engohang AE, Kombila M, , 2012. Complicated malaria and other severe febrile illness in a pediatric ward in Libreville, Gabon. BMC Infect Dis 12: 216.[Crossref] [Google Scholar]
  5. Nkoghe D, Kassa RF, Bisvigou U, Caron M, Grard G, Leroy EM, , 2012. No clinical or biological difference between chikungunya and dengue fever during the 2010 Gabonese outbreak. Infect Dis Rep 4: e5.[Crossref] [Google Scholar]
  6. Perret JL, Velasque L, Morillon M, Martet G, , 1996. Leptospirosis: a cause of pseudo-malarial fever in Gabon. Bull Soc Pathol Exot 89: 217219. [Google Scholar]
  7. Gendrel D, Kombila M, Beaudoin-Leblevec G, Richard-Lenoble D, , 1994. Nontyphoidal salmonellal septicemia in Gabonese children infected with Schistosoma intercalatum . Clin Infect Dis 18: 103105.[Crossref] [Google Scholar]
  8. Alabi AS, Frielinghaus L, Kaba H, Kosters K, Huson MA, Kahl BC, Peters G, Grobusch MP, Issifou S, Kremsner PG, Schaumburg F, , 2013. Retrospective analysis of antimicrobial resistance and bacterial spectrum of infection in Gabon, central Africa. BMC Infect Dis 13: 455.[Crossref] [Google Scholar]
  9. Fenollar F, Mediannikov O, Socolovschi C, Bassene H, Diatta G, Richet H, Tall A, Sokhna C, Trape JF, Raoult D, , 2010. Tropheryma whipplei bacteremia during fever in rural west Africa. Clin Infect Dis 51: 515521.[Crossref] [Google Scholar]
  10. Angelakis E, Mediannikov O, Socolovschi C, Mouffok N, Bassene H, Tall A, Niangaly H, Doumbo O, Znazen A, Sarih M, Sokhna C, Raoult D, , 2014. Coxiella burnetii-positive PCR in febrile patients in rural and urban Africa. Int J Infect Dis 28: 107110 [Crossref] [Google Scholar]
  11. Mediannikov O, Diatta G, Fenollar F, Sokhna C, Trape JF, Raoult D, , 2010. Tick-borne rickettsioses, neglected emerging diseases in rural Senegal. PLoS Negl Trop Dis 4: e821.[Crossref] [Google Scholar]
  12. Mediannikov O, Socolovschi C, Bassene H, Diatta G, Ratmanov P, Fenollar F, Sokhna C, Raoult D, , 2014. Borrelia crocidurae infection in acutely febrile patients, Senegal. Emerg Infect Dis 20: 13351338.[Crossref] [Google Scholar]
  13. Mediannikov O, Socolovschi C, Edouard S, Fenollar F, Mouffok N, Bassene H, Diatta G, Tall A, Niangaly H, Doumbo O, Lekana-Douki JB, Znazen A, Sarih M, Ratmanov P, Richet H, Ndiath MO, Sokhna C, Parola P, Raoult D, , 2013. Common epidemiology of Rickettsia felis infection and malaria, Africa. Emerg Infect Dis 19: 17751783.[Crossref] [Google Scholar]
  14. Sokhna C, Mediannikov O, Fenollar F, Bassene H, Diatta G, Tall A, Trape JF, Drancourt M, Raoult D, , 2013. Point-of-care laboratory of pathogen diagnosis in rural Senegal. PLoS Negl Trop Dis 7: e1999.[Crossref] [Google Scholar]
  15. Elbir H, Raoult D, Drancourt M, , 2013. Relapsing fever borreliae in Africa. Am J Trop Med Hyg 89: 288292.[Crossref] [Google Scholar]
  16. Richards AL, Jiang J, Omulo S, Dare R, Abdirahman K, Ali A, Sharif SK, Feikin DR, Breiman RF, Njenga MK, , 2010. Human infection with Rickettsia felis, Kenya. Emerg Infect Dis 16: 10811086.[Crossref] [Google Scholar]
  17. Maina AN, Knobel DL, Jiang J, Halliday J, Feikin DR, Cleaveland S, Ng'ang'a Z, Junghae M, Breiman RF, Richards AL, Njenga MK, , 2012. Rickettsia felis infection in febrile patients, western Kenya, 2007–2010. Emerg Infect Dis 18: 328331.[Crossref] [Google Scholar]
  18. Cutler SJ, Bonilla EM, Singh RJ, , 2010. Population structure of east African relapsing fever Borrelia spp. Emerg Infect Dis 16: 10761080.[Crossref] [Google Scholar]
  19. Matsumoto K, Parola P, Rolain JM, Jeffery K, Raoult D, , 2007. Detection of “Rickettsia sp. strain Uilenbergi” and “Rickettsia sp. strain Davousti” in Amblyomma tholloni ticks from elephants in Africa. BMC Microbiol 7: 74.[Crossref] [Google Scholar]
  20. Rolain JM, Bourry O, Davoust B, Raoult D, , 2005. Bartonella quintana and Rickettsia felis in Gabon. Emerg Infect Dis 11: 17421744.[Crossref] [Google Scholar]
  21. Socolovschi C, Pages F, Raoult D, , 2012. Rickettsia felis in Aedes albopictus mosquitoes, Libreville, Gabon. Emerg Infect Dis 18: 16871689.[Crossref] [Google Scholar]
  22. Gundi VA, Bourry O, Davous B, Raoult D, La Scola B, , 2004. Bartonella clarridgeiae and B. henselae in dogs, Gabon. Emerg Infect Dis 10: 22612262. [Google Scholar]
  23. Radulovic S, Higgins JA, Jaworski DC, Dasch GA, Azad AF, , 1995. Isolation, cultivation, and partial characterization of the ELB agent associated with cat fleas. Infect Immun 63: 48264829. [Google Scholar]
  24. Schaumburg F, Biallas B, Ngoune FE, Alabi AS, Mordmuller B, Kremsner PG, Grobusch MP, Lell B, van der Linden M, Peters G, Adegnika AA, , 2013. Carriage of encapsulated bacteria in Gabonese children with sickle cell anaemia. Clin Microbiol Infect 19: 235241.[Crossref] [Google Scholar]
  25. Bottieau E, Verbruggen E, Aubry C, Socolovschi C, Vlieghe E, , 2012. Meningoencephalitis complicating relapsing fever in traveler returning from Senegal. Emerg Infect Dis 18: 697698.[Crossref] [Google Scholar]
  26. Mawili-Mboumba DP, Bouyou-Akotet MK, Kendjo E, Nzamba J, Medang MO, Mbina JR, Kombila M, , 2013. Increase in malaria prevalence and age of at risk population in different areas of Gabon. Malar J 12: 3.[Crossref] [Google Scholar]
  27. Bouyou-Akotet MK, Offouga CL, Mawili-Mboumba DP, Essola L, Madoungou B, Kombila M, , 2014. Falciparum malaria as an emerging cause of fever in adults living in Gabon, central Africa. Biomed Res Int 2014: 351281.[Crossref] [Google Scholar]
  28. Nkoghe D, Akue JP, Gonzalez JP, Leroy EM, , 2011. Prevalence of Plasmodium falciparum infection in asymptomatic rural Gabonese populations. Malar J 10: 33.[Crossref] [Google Scholar]
  29. Morpeth SC, Ramadhani HO, Crump JA, , 2009. Invasive non-typhi Salmonella disease in Africa. Clin Infect Dis 49: 606611.[Crossref] [Google Scholar]
  30. Nadjm B, Amos B, Mtove G, Ostermann J, Chonya S, Wangai H, Kimera J, Msuya W, Mtei F, Dekker D, Malahiyo R, Olomi R, Crump JA, Whitty CJ, Reyburn H, , 2010. WHO guidelines for antimicrobial treatment in children admitted to hospital in an area of intense Plasmodium falciparum transmission: prospective study. BMJ 340: c1350.[Crossref] [Google Scholar]
  31. Biggs HM, Lester R, Nadjm B, Mtove G, Todd JE, Kinabo GD, Philemon R, Amos B, Morrissey AB, Reyburn H, Crump JA, , 2014. Invasive Salmonella infections in areas of high and low malaria transmission intensity in Tanzania. Clin Infect Dis 58: 638647.[Crossref] [Google Scholar]
  32. D'Acremont V, Kilowoko M, Kyungu E, Philipina S, Sangu W, Kahama-Maro J, Lengeler C, Cherpillod P, Kaiser L, Genton B, , 2014. Beyond malaria—causes of fever in outpatient Tanzanian children. N Engl J Med 370: 809817.[Crossref] [Google Scholar]
  33. Cevey-Macherel M, Galetto-Lacour A, Gervaix A, Siegrist CA, Bille J, Bescher-Ninet B, Kaiser L, Krahenbuhl JD, Gehri M, , 2009. Etiology of community-acquired pneumonia in hospitalized children based on WHO clinical guidelines. Eur J Pediatr 168: 14291436.[Crossref] [Google Scholar]
  34. Peltola V, Waris M, Osterback R, Susi P, Hyypia T, Ruuskanen O, , 2008. Clinical effects of rhinovirus infections. J Clin Virol 43: 411414.[Crossref] [Google Scholar]
  35. Crump JA, Morrissey AB, Nicholson WL, Massung RF, Stoddard RA, Galloway RL, Ooi EE, Maro VP, Saganda W, Kinabo GD, Muiruri C, Bartlett JA, , 2013. Etiology of severe non-malaria febrile illness in northern Tanzania: a prospective cohort study. PLoS Negl Trop Dis 7: e2324.[Crossref] [Google Scholar]
  36. Ayoola OO, Adeyemo AA, Osinusi K, , 2005. Concurrent bacteraemia and malaria in febrile Nigerian infants. Trop Doct 35: 3436.[Crossref] [Google Scholar]
  37. Animut A, Mekonnen Y, Shimelis D, Ephraim E, , 2009. Febrile illnesses of different etiology among outpatients in four health centers in northwestern Ethiopia. Jpn J Infect Dis 62: 107110. [Google Scholar]
  38. Mediannikov O, Socolovschi C, Million M, Sokhna C, Bassene H, Diatta G, Fenollar F, Raoult D, , 2014. Molecular identification of pathogenic bacteria in eschars from acute febrile patients, Senegal. Am J Trop Med Hyg 91: 10151019.[Crossref] [Google Scholar]
  39. Msaki BP, Mshana SE, Hokororo A, Mazigo HD, Morona D, , 2012. Prevalence and predictors of urinary tract infection and severe malaria among febrile children attending Makongoro health centre in Mwanza city, North-Western Tanzania. Arch Public Health 70: 4.[Crossref] [Google Scholar]
  40. Raoult D, Roblot F, Rolain JM, Besnier JM, Loulergue J, Bastides F, Choutet P, , 2006. First Isolation of Bartonella alsatica from a valve of a patient with endocarditis. J Clin Microbiol 44: 278279.[Crossref] [Google Scholar]
  41. Parola P, Ryelandt J, Mangold AJ, Mediannikov O, Guglielmone AA, Raoult D, , 2011. Relapsing fever Borrelia in ornithodoros ticks from Bolivia. Ann Trop Med Parasitol 105: 407411.[Crossref] [Google Scholar]
  42. Keita AK, Bassene H, Tall A, Sokhna C, Ratmanov P, Trape JF, Raoult D, Fenollar F, , 2011. Tropheryma whipplei: a common bacterium in rural Senegal. PLoS Negl Trop Dis 5: e1403.[Crossref] [Google Scholar]
  43. Mediannikov O, Fenollar F, Socolovschi C, Diatta G, Bassene H, Molez JF, Sokhna C, Trape JF, Raoult D, , 2010. Coxiella burnetii in humans and ticks in rural Senegal. PLoS Negl Trop Dis 4: e654.[Crossref] [Google Scholar]
  44. González-Escalona N, Hammack TS, Russell M, Jacobson AP, De Jesús AJ, Brown EW, Lampel KA, , 2009. Detection of Live Salmonella sp. cells in produce by a TaqMan-based quantitative reverse transcriptase real-time PCR targeting invA mRNA. Appl Environ Microbiol 75: 37143720.[Crossref] [Google Scholar]
  45. Ruiz-Pérez de Pipaón M, Torres-Sánchez MJ, Arroyo-Pedrero LA, Prados-Blanco T, Palomares-Folía JC, Aznar-Martín J, , 2005. Detection of methicillin resistance and identification of Staphylococcus spp. from positive blood culture bottles using the mecA and nucA genes with the LightCycler system. Enferm Infecc Microbiol Clin 23: 208212.[Crossref] [Google Scholar]
  46. Carvalho M da G, Tondella ML, McCaustland K, Weidlich L, McGee L, Mayer LW, Steigerwalt A, Whaley M, Facklam RR, Fields B, Carlone G, Ades EW, Dagan R, Sampson JS, , 2007. Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA. J Clin Microbiol 45: 24602466.[Crossref] [Google Scholar]
  47. Saravolatz LD, Johnson L, Galloway L, Manzor O, Pawlak J, Belian B, , 2007. Detection of Streptococcus pneumoniae colonisation in respiratory tract secretions of military personnel. Clin Microbiol Infect 13: 932936.[Crossref] [Google Scholar]
  48. Xiong L, Kong F, Yang Y, Cheng J, Gilbert GL, , 2006. Use of PCR and reverse line blot hybridization macroarray based on 16S–23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 Mycobacterium species. J Clin 44: 35443550. [Google Scholar]
  49. Thaipadungpanit J, Chierakul W, Wuthiekanun V, Limmathurotsakul D, Amornchai P, Boonslip S, Smythe LD, Limpaiboon R, Hoffmaster AR, Day NP, Peacock SJ, , 2011. Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: a case-control study. PLoS One 6: e16236.[Crossref] [Google Scholar]

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  • Received : 04 Nov 2014
  • Accepted : 07 Feb 2015
  • Published online : 06 May 2015

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