1921
Volume 93, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Routine national notifications of dengue cases typically do not reflect the true dengue situation due to large proportion of unreported cases. Serosurveys, when conducted periodically, could shed light on the true dengue infections in the population. To determine the magnitude of dengue infections of the adult population in Singapore following the outbreak in 2007, we performed a cross-sectional study on blood donor samples from December 2009 to February 2010. The residual blood of 3,995 donors (aged 16–60 years) was screened for the presence of dengue-specific immunoglobulin G (IgG) and IgM using enzyme-linked immunosorbent assay (ELISA) kits. The age-weighted IgG prevalence of residents was 50.8% ( = 3,627, 95% confidence interval [CI] = 49.4–52.3%). Dengue IgG prevalence increased with age, with the lowest in 16–20 years age group (16.1%) and the highest in 56–60 years age group (86.6%). Plaque reduction neutralization test (PRNT) on samples of young resident adults (aged 16–30 years) revealed lower prevalence of neutralizing antibodies to each serotype, ranging from 5.4% to 20.3% compared with the older age groups. The level of exposure to dengue among the young adults is relatively low despite the endemicity of the disease in Singapore. It partially explains the population's susceptibility to explosive outbreaks and the high incidence rate among young adults.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.14-0671
2015-07-08
2017-09-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/93/1/40.html?itemId=/content/journals/10.4269/ajtmh.14-0671&mimeType=html&fmt=ahah

References

  1. World Health Organization, 2012. Global Strategy for Dengue Prevention and Control. Available at: http://www.who.int/denguecontrol/9789241504034/en. Accessed January 21, 2013.
  2. World Health Organization, 2012. Dengue and Severe Dengue. Fact sheet No. 117. Available at: http://www.who.int/mediacentre/factsheets/fs117/en/. Accessed January 21, 2013.
  3. Murray NE, Quam MB, Wilder-Smith A, , 2013. Epidemiology of dengue: past, present and future prospects. Clin Epidemiol 5: 299309.
  4. Wichmann O, Yoon IK, Vong S, Limkittikul K, Gibbons RV, Mammen MP, Ly S, Buchy P, Sirivichayakul C, Buathong R, Huy R, Letson GW, Sabchareon A, , 2011. Dengue in Thailand and Cambodia: an assessment of the degree of underrecognized disease burden based on reported cases. PLoS Negl Trop Dis 5: e996.[Crossref]
  5. Standish K, Kuan G, Aviles W, Balmaseda A, Harris E, , 2010. High dengue case capture rate in four years of a cohort study in Nicaragua compared to national surveillance data. PLoS Negl Trop Dis 4: e633.[Crossref]
  6. Burke DS, Nisalak A, Johnson DE, Scott RM, , 1988. A prospective study of dengue infections in Bangkok. Am J Trop Med Hyg 38: 172180.
  7. Yap G, Li C, Mutalib A, Lai YL, Ng LC, , 2013. High rates of inapparent dengue in older adults in Singapore. Am J Trop Med Hyg 88: 10651069.[Crossref]
  8. Goh KT, Yamazaki S, , 1987. Serological survey on dengue virus infection in Singapore. Trans R Soc Trop Med Hyg 81: 687689.[Crossref]
  9. Goh KT, , 1995. Changing epidemiology of dengue in Singapore. Lancet 346: 1098.[Crossref]
  10. Koh BK, Ng LC, Kita Y, Tang CS, Ang LW, Wong KY, James L, Goh KT, , 2008. The 2005 dengue epidemic in Singapore: epidemiology, prevention and control. Ann Acad Med Singapore 37: 538545.
  11. Ministry of Health, Singapore, 2005. Communicable Diseases Surveillance in Singapore 2005. Singapore: Ministry of Health, 2547.
  12. Ministry of Health, Singapore, 2006. Communicable Diseases Surveillance in Singapore 2006. Singapore: Ministry of Health, 2543.
  13. Ministry of Health, Singapore, 2007. Communicable Diseases Surveillance in Singapore 2007. Singapore: Ministry of Health, 2143.
  14. Ler TS, Ang LW, Yap G, Ng LC, Tai JC, James L, Goh KT, , 2011. Epidemiological characteristics of the 2005 and 2007 dengue epidemics in Singapore: similarities and distinctions. Western Pac Surveill Response J 2: 2429.
  15. Committee on Epidemic Diseases, Singapore, 2000. Seroprevalence of dengue virus infection in Singapore. Epidemiol News Bull 26: 3031.
  16. Wilder-Smith A, Yoksan S, Earnest A, Subramaniam R, Paton NI, , 2005. Serological evidence for the co-circulation of multiple dengue virus serotypes in Singapore. Epidemiol Infect 133: 667671.[Crossref]
  17. Chow VTK, Lim CS, Phoon MC, Tan HC, , 2005. A Seroprevalence survey of dengue virus infection in healthy Singapore University undergraduates by enzyme immunoassay and plaque reduction neutralization test. Dengue Bull 29: 97105.
  18. Yew YW, Ye T, Ang LW, Ng LC, Yap G, James L, Chew SK, Goh KT, , 2009. Seroepidemiology of dengue virus infection among adults in Singapore. Ann Acad Med Singapore 38: 667675.
  19. Morens DM, Halstead SB, Repik PM, Putvatana R, Raybourne N, , 1985. Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. J Clin Microbiol 22: 250254.
  20. Russell PK, Nisalak A, Sukhavachana P, Vivona S, , 1967. A plaque reduction test for dengue virus neutralizing antibodies. J Immunol 99: 285290.
  21. Yew YW, Ye T, Ang LW, Ng LC, Yap G, James L, Chew SK, Goh KT, , 2009. Seroepidemiology of dengue virus infection among adults in Singapore. Ann Acad Med Singapore 38: 667675.
  22. Chungue E, Marche G, Plichart R, Boutin JP, Roux J, , 1989. Comparison of immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA) and haemagglutination inhibition (HI) test for the detection of dengue antibodies. Prevalence of dengue IgG-ELISA antibodies in Tahiti. Trans R Soc Trop Med Hyg 83: 708711.[Crossref]
  23. McBride WJ, Mullner H, LaBrooy JT, Wronski I, , 1998. The 1993 dengue 2 epidemic in north Queensland: a serosurvey and comparison of hemagglutination inhibition with an ELISA. Am J Trop Med Hyg 59: 457461.
  24. Ministry of Health, Singapore, 2004. Communicable Diseases Surveillance in Singapore, 2004. Singapore: Ministry of Health, 2738.
  25. Ministry of Health, Singapore, 2013. Communicable Diseases Surveillance in Singapore, 2013. Singapore: Ministry of Health, 5265.
  26. Ministry of Health, Singapore, 2014. Prevalence of past dengue virus infection among children and adults in Singapore. Epidemiol News Bull 40: 102106.
  27. Thai KT, Nishiura H, Hoang PL, Tran NT, Phan GT, Le HQ, Tran BQ, Nguyen NV, de Vries PJ, , 2011. Age-specificity of clinical dengue during primary and secondary infections. PLoS Negl Trop Dis 5: e1180.[Crossref]
  28. Malavige GN, Fernando S, Aaskov J, Sivayogan S, Dissanayaka T, Peelawattage M, Dabare M, , 2006. Seroprevalence of anti-dengue virus antibodies in children in Colombo District, Sri Lanka. Dengue Bull 30: 6871.
  29. Thai KT, Binh TQ, Giao PT, Phuong HL, Hung le Q, Van Nam N, Nga TT, Groen J, Nagelkerke N, de Vries PJ, , 2005. Seroprevalence of dengue antibodies, annual incidence and risk factors among children in southern Vietnam. Trop Med Int Health 10: 379386.[Crossref]
  30. Brunkard JM, Robles Lopez JL, Ramirez J, Cifuentes E, Rothenberg SJ, Hunsperger EA, Moore CG, Brussolo RM, Villarreal NA, Haddad BM, , 2007. Dengue fever seroprevalence and risk factors, Texas–Mexico border, 2004. Emerg Infect Dis 13: 14771483.[Crossref]
  31. Balmaseda A, Hammond SN, Tellez Y, Imhoff L, Rodriguez Y, Saborio SI, Mercado JC, Perez L, Videa E, Almanza E, Kuan G, Reyes M, Saenz L, Amador JJ, Harris E, , 2006. High seroprevalence of antibodies against dengue virus in a prospective study of schoolchildren in Managua, Nicaragua. Trop Med Int Health 11: 935942.[Crossref]
  32. Yamashiro T, Disla M, Petit A, Taveras D, Castro-Bello M, Lora-Orste M, Vardez S, Cesin AJ, Garcia B, Nishizono A, , 2004. Seroprevalence of IgG specific for dengue virus among adults and children in Santo Domingo, Dominican Republic. Am J Trop Med Hyg 71: 138143.
  33. Braga C, Luna CF, Martelli CM, de Souza WV, Cordeiro MT, Alexander N, de Albuquerque M de F, Junior JC, Marques ET, , 2010. Seroprevalence and risk factors for dengue infection in socio-economically distinct areas of Recife, Brazil. Acta Trop 113: 234240.[Crossref]
  34. Muhammad Azami NA, Salleh SA, Neoh HM, Syed Zakaria SZ, Jamal R, , 2011. Dengue epidemic in Malaysia: not a predominantly urban disease anymore. BMC Res Notes 4: 216.[Crossref]
  35. Lee KS, Lai YL, Lo S, Barkham T, Aw P, Ooi PL, Tai JC, Hibberd M, Johansson P, Khoo SP, Ng LC, , 2010. Dengue virus surveillance for early warning, Singapore. Emerg Infect Dis 16: 847849.[Crossref]
  36. Ministry of Health, Singapore, 2012. Review of dengue serotype surveillance programme in Singapore. Epidemiol News Bull 38: 5462.
  37. Schwartz E, Mileguir F, Grossman Z, Mendelson E, , 2000. Evaluation of ELISA-based sero-diagnosis of dengue fever in travelers. J Clin Virol 19: 169173.[Crossref]
  38. Cordeiro MT, Braga-Neto U, Nogueira RM, Marques ET, Jr, 2009. Reliable classifier to differentiate primary and secondary acute dengue infection based on IgG ELISA. PLoS One 4: e4945.[Crossref]
  39. Koh YL, Tan BH, Loh JJ, Ooi EE, Su SY, Hsu LY, , 2006. Japanese encephalitis, Singapore. Emerg Infect Dis 12: 525526.[Crossref]
  40. World Health Organization, 2014. World Health Statistics 2014. Available at: http://www.who.int/gho/publications/world_health_statistics/2014/en/. Accessed August 23, 2014.
  41. Teo D, Ng LC, Lam S, , 2009. Is dengue a threat to the blood supply? Transfus Med 19: 6677.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.14-0671
Loading
/content/journals/10.4269/ajtmh.14-0671
Loading

Data & Media loading...

Supplementary Data

Supplementary PDF

  • Received : 23 Oct 2014
  • Accepted : 24 Mar 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error