Volume 92, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In Tunisia, malaria transmission has been interrupted since 1980. However, the growing number of imported cases and the persistence of putative vectors stress the need for additional studies to assess the risk of malaria resurgence in the country. In this context, our aim was to update entomological data concerning mosquitoes in Tunisia. From May to October of 2012, mosquito larval specimens were captured in 60 breeding sites throughout the country and identified at the species level using morphological keys. Environmental parameters of the larval habitats were recorded. Specimens belonging to the complex were further identified to sibling species by the ribosomal deoxyribonucleic acid (rDNA)–internal transcribed spacer 2 (ITS2) polymerase chain reaction (PCR) technique. In total, 647 larvae were collected from 25 habitats. Four species, including , , , and , were morphologically identified. rDNA-ITS2 PCR confirmed that is the sole member of the complex in Tunisia. was collected throughout northern and central Tunisia, and it was highly associated with rural habitat, clear water, and sunlight areas. Larvae of and existed separately or together and were collected in southern Tunisia in similar types of breeding places.


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  • Received : 12 Aug 2014
  • Accepted : 04 Nov 2014

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