Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In September 2009, a cross-sectional study was conducted to evaluate parasitic infections in a child care center in Khlong Toei, Bangkok, Thailand. Of 503 children and staff members, 258 (51.3%) stool samples and questionnaires were obtained. The most common parasitic infection was sp. (13.6%). sp. subtype 3 was predominantly found (80.0%), followed by subtypes 2 (12.0%) and 1 (8.0%). The prevalence of infection varied among different age groups. The prevalence of infection in non-HIV-infected children aged < 10 and 10–19 years were 14.5% and 10.3%, respectively, which were not significantly different. All 31 HIV-infected children were not infected with sp. The most likely reason could be the result of properly using prevention measures for this specific group.


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  1. Saksirisampant W, Nuchprayoon S, Wiwanitkit V, Yenthakam S, Ampavasiri A, , 2003. Intestinal parasitic infestations among children in an orphanage in Pathum Thani province. J Med Assoc Thai 86: 263270.
  2. Boondit J, Pipatsatitpong D, Mungthin M, Taamasri P, Tan-ariya P, Naaglor T, Leelayoova S, , 2014. Incidence and risk factors of Blastocystis infection in orphans at the Babies' Home, Nonthaburi Province, Thailand. J Med Assoc Thai 97 (Suppl 2): S52S59.
  3. Thathaisong U, Siripattanapipong S, Mungthin M, Pipatsatitpong D, Tan-Ariya P, Naaglor T, Leelayoova S, , 2012. Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand. Am J Trop Med Hyg 88: 352358.[Crossref]
  4. Jantermtor S, Pinlaor P, Sawadpanich K, Pinlaor S, Sangka A, Wilailuckana C, Wongsena W, Yoshikawa H, , 2013. Subtype identification of Blastocystis spp. isolated from patients in a major hospital in northeastern Thailand. Parasitol Res 112: 17811786.[Crossref]
  5. Leelayoova S, Siripattanapipong S, Thathaisong U, Naaglor T, Taamasri P, Piyaraj P, Mungthin M, , 2008. Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand. Am J Trop Med Hyg 79: 401406.
  6. Al FD, Hokelek M, , 2007. Is Blastocystis hominis an opportunist agent? Turkiye Parazitol Derg 31: 2836.
  7. Hussein EM, Hussein AM, Eida MM, Atwa MM, , 2008. Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats. Parasitol Res 102: 853860.[Crossref]
  8. Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Ahmed A, Surin J, Mak JW, , 2012. Drinking water is a significant predictor of Blastocystis infection among rural Malaysian primary schoolchildren. Parasitology 139: 10141020.[Crossref]
  9. Lee IL, Tan TC, Tan PC, Nanthiney DR, Biraj MK, Surendra KM, Suresh KG, , 2012. Predominance of Blastocystis sp. subtype 4 in rural communities, Nepal. Parasitol Res 110: 15531562.[Crossref]
  10. Yoshikawa H, Abe N, Iwasawa M, Kitano S, Nagano I, Wu Z, Takahashi Y, , 2000. Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities. J Clin Microbiol 38: 13241330.
  11. Institute of Nutrition, Mahidol University, 2009. INMU ThaiGrowth. Available at: http://www.inmu.mahidol.ac.th/thaigrowth/. Accessed September 1, 2009.
  12. World Health Organization, 2005. The Treatment of Diarrhoea: A Manual for Physicians and Other Senior Health Workers. Geneva, Switzerland: WHO Press, 343.
  13. Yaicharoen R, Ngrenngarmlert W, Wongjindanon N, Sripochang S, Kiatfuengfoo R, , 2006. Infection of Blastocystis hominis in primary schoolchildren from Nakhon Pathom Province, Thailand. Trop Biomed 23: 117122.
  14. Rayan P, Verghese S, McDonnell P, , 2010. Geographical location and age affects the incidence of parasitic infestations in school children. Indian J Pathol Microbiol 53: 498502.[Crossref]
  15. Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M, , 2002. In-vitro cultivation: a sensitive method for detecting Blastocystis hominis . Ann Trop Med Parasitol 96: 803807.[Crossref]
  16. Roberts T, Barratt J, Harkness J, Ellis J, Stark D, , 2011. Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples. Am J Trop Med Hyg 84: 308312.[Crossref]
  17. El Safadi D, Gaayeb L, Meloni D, Cian A, Poirier P, Wawrzyniak I, Delbac F, Dabboussi F, Delhaes L, Seck M, Hamze M, Riveau G, Viscogliosi E, , 2014. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide. BMC Infect Dis 14: 164174.[Crossref]
  18. Yoshikawa H, Dogruman-Ai F, Turk S, Kustimur S, Balaban N, Sultan N, , 2011. Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples. Parasitol Res 109: 10451050.[Crossref]
  19. Yaicharoen R, Sripochang S, Sermsart B, Pidetcha P, , 2005. Prevalence of Blastocystis hominis infection in asymptomatic individuals from Bangkok, Thailand. Southeast Asian J Trop Med Public Health 36: 1720.
  20. Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Ahmed A, Surin J, Mak JW, , 2012. Drinking water is a significant predictor of Blastocystis infection among rural Malaysian primary schoolchildren. Parasitology 139: 10141020.[Crossref]
  21. Anuar TS, Ghani MK, Azreen SN, Salleh FM, Moktar N, , 2013. Blastocystis infection in Malaysia: evidence of waterborne and human-to-human transmissions among the Proto-Malay, Negrito and Senoi tribes of Orang Asli. Parasit Vectors 6: 4051.[Crossref]
  22. Suresh K, Venilla G, Tan T, Rohela M, , 2009. In vivo encystation of Blastocystis hominis . Parasitol Res 104: 13731380.[Crossref]
  23. Zhou XB, Zhang X, Qiao JY, Cai J, Cheng S, Yuan Y, Li J, , 2010. Encystation-survival of Blastocystis hominis in immunocompetent mice abdomen cavity. Parasitol Res 106: 13151320.[Crossref]
  24. Alemu A, Shiferaw Y, Getnet G, Yalew A, Addis Z, , 2011. Opportunistic and other intestinal parasites among HIV/AIDS patients attending Gambi higher clinic in Bahir Dar city, north west Ethiopia. Asian Pac J Trop Med 4: 661665.[Crossref]
  25. Kulkarni SV, Kairon R, Sane SS, Padmawar PS, Kale VA, Thakar MR, Mehendale SM, Risbud AR, , 2009. Opportunistic parasitic infections in HIV/AIDS patients presenting with diarrhoea by the level of immunesuppression. Indian J Med Res 130: 6366.
  26. Hailemariam G, Kassu A, Abebe G, Abate E, Damte D, Mekonnen E, Ota F, , 2004. Intestinal parasitic infections in HIV/AIDS and HIV seronegative individuals in a teaching hospital, Ethiopia. Jpn J Infect Dis 57: 4143.
  27. Teklemariam Z, Abate D, Mitiku H, Dessie Y, , 2013. Prevalence of intestinal parasitic infection among HIV positive persons who are naive and on antiretroviral treatment in Hiwot Fana Specialized University Hospital, eastern Ethiopia. ISRN AIDS 11: 324329.
  28. Adamu H, Wegayehu T, Petros B, , 2013. High prevalence of diarrhoegenic intestinal parasite infections among non-ART HIV patients in Fitche Hospital, Ethiopia. PLoS One 8: 72634.[Crossref]
  29. Tian LG, Chen JX, Wang TP, Cheng GJ, Steinmann P, Wang FF, Cai YC, Yin XM, Guo J, Zhou L, Zhou XN, , 2012. Co-infection of HIV and intestinal parasites in rural area of China. Parasit Vectors 5: 36.[Crossref]
  30. Nielsen NO, Simonsen PE, Magnussen P, Magesa S, Friis H, , 2006. Cross-sectional relationship between HIV, lymphatic filariasis and other parasitic infections in adults in coastal northeastern Tanzania. Trans R Soc Trop Med Hyg 100: 543550.[Crossref]
  31. Nielsen NO, Friis H, Magnussen P, Krarup H, Magesa S, , 2007. Co-infection with subclinical HIV and Wuchereria bancrofti, and the role of malaria and hookworms, in adult Tanzanians: infection intensities, CD4/CD8 counts and cytokine responses. Trans R Soc Trop Med Hyg 101: 602612.[Crossref]
  32. Pipatsatitpong D, Rangsin R, Leelayoova S, Naaglor T, Mungthin M, , 2012. Incidence and risk factors of Blastocystis infection in an orphanage in Bangkok, Thailand. Parasit Vectors 5: 37.[Crossref]
  33. Thathaisong U, Worapong J, Mungthin M, Tan-Ariya P, Viputtigul K, Sudatis A, Noonai A, Leelayoova S, , 2003. Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis . J Clin Microbiol 41: 967975.[Crossref]
  34. Parkar U, Traub RJ, Kumar S, Mungthin M, Vitali S, Leelayoova S, Morris K, Thompson RC, , 2007. Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitology 134: 359367.[Crossref]
  35. Tan TC, Suresh KG, Smith HV, , 2008. Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitol Res 104: 8593.[Crossref]
  36. Elwakil HS, Talaat RM, , 2009. Genetic analysis of Blastocystis hominis isolated from symptomatic and asymptomatic human hosts in Egypt. J Egypt Soc Parasitol 39: 99109.

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  • Received : 06 Aug 2014
  • Accepted : 11 Apr 2015

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