Volume 92, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In this study, three molecular assays (real-time multiplex polymerase chain reaction [PCR], merozoite surface antigen gene []-multiplex PCR, and the PlasmoNex Multiplex PCR Kit) have been developed for diagnosis of species. In total, 52 microscopy-positive and 20 malaria-negative samples were used in this study. We found that real-time multiplex PCR was the most sensitive for detecting and . The -multiplex PCR assay and the PlasmoNex Multiplex PCR Kit were equally sensitive for diagnosing infection, whereas the PlasmoNex Multiplex PCR Kit and real-time multiplex PCR showed similar sensitivity for detecting . The three molecular assays displayed 100% specificity for detecting malaria samples. We observed no significant differences between -multiplex PCR and the PlasmoNex multiplex PCR kit (McNemar's test: = 0.1489). However, significant differences were observed comparing real-time multiplex PCR with the PlasmoNex Multiplex PCR Kit (McNemar's test: = 0.0044) or real-time multiplex PCR with -multiplex PCR (McNemar's test: = 0.0012).


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  • Received : 19 May 2014
  • Accepted : 17 Sep 2014
  • Published online : 07 Jan 2015

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