1921
Volume 92, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Accurate diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is required to avoid the risk of acute hemolysis associated with 8-aminoquinoline treatment. The performance of the BinaxNOW G6PD test compared with the quantitative spectrophotometric analysis of G6PD activity was assessed in 356 infected subjects in Brazil, Peru, Thailand, and India. In the quantitative assay, the median G6PD activity was 8.81 U/g hemoglobin (range = 0.05–20.19), with 11 (3%) subjects identified as deficient. Sensitivity of the BinaxNOW G6PD to detect deficient subjects was 54.5% (6 of 11), and specificity was 100% (345 of 345). Room temperatures inadvertently falling outside the range required to perform the rapid test (18–25°C) together with subtlety of color change and insufficient training could partially explain the low sensitivity found. Ensuring safe use of 8-aminoquinolines depends on additional development of simple, highly sensitive G6PD deficiency diagnostic tests suitable for routine use in malaria-endemic areas.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.14-0298
2015-01-07
2017-11-17
Loading full text...

Full text loading...

/deliver/fulltext/14761645/92/1/22.html?itemId=/content/journals/10.4269/ajtmh.14-0298&mimeType=html&fmt=ahah

References

  1. Battle KE, Gething PW, Elyazar IR, Moyes CL, Sinka ME, Howes RE, Guerra CA, Price RN, Baird KJ, Hay SI, , 2012. The global public health significance of Plasmodium vivax . Adv Parasitol 80: 1111.[Crossref]
  2. World Health Organization, 2010. Guidelines for the Treatment of Malaria. Geneva: WHO Press.
  3. Llanos-Cuentas A, Lacerda MV, Rueangweerayut R, Krudsood S, Gupta SK, Kochar SK, Arthur P, Chuenchom N, Möhrle JJ, Duparc S, Ugwuegbulam C, Kleim JP, Carter N, Green JA, Kellam L, , 2013. Tafenoquine plus chloroquine for the treatment and relapse prevention of Plasmodium vivax malaria (DETECTIVE): a multicentre, double-blind, randomised, phase 2b dose-selection study. Lancet 383: 10491058.[Crossref]
  4. Howes RE, Piel FB, Patil AP, Nyangiri OA, Gething PW, Dewi M, Hogg MM, Battle KE, Padilla CD, Baird JK, Hay SI, , 2012. G6PD deficiency prevalence and estimates of affected populations in malaria endemic countries: a geostatistical model-based map. PLoS Med 9: e1001339.[Crossref]
  5. Burgoine KL, Bancone G, Nosten F, , 2010. The reality of using primaquine. Malar J 9: 376.[Crossref]
  6. Cappellini MD, Fiorelli G, , 2008. Glucose-6-phosphate dehydrogenase deficiency. Lancet 371: 6474.[Crossref]
  7. Beutler E, Yeh M, Fairbanks VF, , 1962. The normal human female as a mosaic of X-chromosome activity: studies using the gene for G-6-PD-deficiency as a marker. Proc Natl Acad Sci USA 48: 916.[Crossref]
  8. Domingo GJ, Satyagraha AW, Anvikar A, Baird K, Bancone G, Bansil P, Carter N, Cheng Q, Culpepper J, Eziefula C, Fukuda M, Green J, Hwang J, Lacerda M, McGray S, Menard D, Nosten F, Nuchprayoon I, Oo NN, Bualombai P, Pumpradit W, Qian K, Recht J, Roca A, Satimai W, Sovannaroth S, Vestergaard LS, Von Seidlein L, , 2013. G6PD testing in support of treatment and elimination of malaria: recommendations for evaluation of G6PD tests. Malar J 12: 391.[Crossref]
  9. Luzzatto L, Seneca E, , 2014. G6PD deficiency: a classic example of pharmacogenetics with on-going clinical implications. Br J Haematol 164: 469480.[Crossref]
  10. Shah SS, Diakite SA, Traore K, Diakite M, Kwiatkowski DP, Rockett KA, Wellems TE, Fairhurst RM, , 2012. A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency. Sci Rep 2: 299.[Crossref]
  11. Tantular IS, Kawamoto F, , 2003. An improved, simple screening method for detection of glucose-6-phosphate dehydrogenase deficiency. Trop Med Int Health 8: 569574.[Crossref]
  12. von Seidlein L, Auburn S, Espino F, Shanks D, Cheng Q, McCarthy J, Baird K, Moyes C, Howes R, Ménard D, Bancone G, Winasti-Satyahraha A, Vestergaard LS, Green J, Domingo G, Yeung S, Price R, , 2013. Review of key knowledge gaps in glucose-6-phosphate dehydrogenase deficiency detection with regard to the safe clinical deployment of 8-aminoquinoline treatment regimens: a workshop report. Malar J 12: 112.[Crossref]
  13. Kim S, Nguon C, Guillard B, Duong S, Chy S, Sum S, Nhem S, Bouchier C, Tichit M, Christophel E, Taylor WR, Baird JK, Menard D, , 2011. Performance of the CareStart™ G6PD deficiency screening test, a point-of-care diagnostic for primaquine therapy screening. PLoS ONE 6: e28357.[Crossref]
  14. Tinley KE, Loughlin AM, Jepson A, Barnett ED, , 2010. Evaluation of a rapid qualitative enzyme chromatographic test for glucose-6-phosphate dehydrogenase deficiency. Am J Trop Med Hyg 82: 210214.[Crossref]
  15. Beutler E, , 1994. G6PD deficiency. Blood 84: 36133636.
  16. Beutler E, Blume KG, Kaplan JC, Löhr GW, Ramot B, Valentine WN, , 1977. International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis. Br J Haematol 35: 331340.[Crossref]
  17. LaRue N, Kahn M, Murray M, Leader BT, Bansil P, McGray S, Kalnoky M, Zhang H, Huang H, Jiang H, Domingo G, , 2014. Comparison of quantitative and qualitative tests for glucose-6-phosphate dehydrogenase deficiency. Am J Trop Med Hyg 91: 854861.[Crossref]
  18. Roth EF, Schulman S, Vanderberg J, Olson J, , 1986. Pathways for the reduction of oxidized glutathione in the Plasmodium falciparum-infected erythrocyte: can parasite enzymes replace host red cell glucose-6-phosphate dehydrogenase? Blood 67: 827830.
  19. Thorburn DR, Kuchel PW, , 1987. G6PD in Plasmodium-infected erythrocytes. Blood 69: 700701.
  20. Howes RE, Battle KE, Satyagraha AW, Baird JK, Hay SI, , 2013. G6PD deficiency: global distribution, genetic variants and primaquine therapy. Adv Parasitol 81: 133201.[Crossref]
  21. Leslie T, Moiz B, Mohammad N, Amanzai O, Ur Rasheed H, Jan S, Siddiqi AM, Nasir A, Beg MA, Vink M, , 2013. Prevalence and molecular basis of glucose-6-phosphate dehydrogenase deficiency in Afghan populations: implications for treatment policy in the region. Malar J 12: 230.[Crossref]
  22. Guyatt G, Drummond R, Meade M, Cook D, , 2008. User's Guides to the Medical Literature: A Manual for Evidence-Based Clinical Practice. New York: McGraw Hill.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.14-0298
Loading
/content/journals/10.4269/ajtmh.14-0298
Loading

Data & Media loading...

  • Received : 13 May 2014
  • Accepted : 28 Aug 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error