1921
Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Cases of human visceral leishmaniasis (HVL) were not recorded until recently in the Chaco region of northwestern Argentina. Dogs were surveyed at the sites of infection of two HVL index cases in the Chaco region of Salta province. Canine cases (CanL) were diagnosed by two parasitological methods, two molecular methods targeting mini- and maxicircle DNA, and immunochromatographic dipstick. Among 77 dogs studied, 10 (13%) were found infected with spp. In seven dogs and two humans, the infecting species was typed as () . The same genotype was detected in the human and two of the CanL. Although several diagnostic methods displayed weak or moderate agreement, the concordance values for serology versus maxicircle PCR were very good (Kappa index = 0.84). Sandflies captured in the area were identified as and / ( complex). The focal appearance of leishmaniasis in dogs and humans in a sylvatic region and its relatively low prevalence of infection suggests that () transmission to dogs and humans may, in this region, stem from sylvatic reservoirs.

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2015-08-05
2017-11-20
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References

  1. Souza TD, Turchetti AP, Fujiwara RT, Paixão TA, Santos RL, , 2014. Visceral leishmaniasis in zoo and wildlife. Vet Parasitol 200: 233241 [Review].[Crossref]
  2. Baneth G, Koutinas AF, Solano-Gallego L, Bourdeau P, Ferrer L, , 2008. Canine leishmaniosis-new concepts and insights on an expanding zoonosis. Trends Parasitol 24: 324330 [Review].[Crossref]
  3. Quinnel RJ, Courtenay O, Garcez LM, Dye C, , 1997. Epidemiology of canine leishmaniasis: transmission rates estimated from a cohort study in Amazon Brazil. Parasitology 115: 143156.[Crossref]
  4. Salomón OD, Sinagra A, Nevot MC, Barberian G, Paulin P, Estevez JO, Riarte A, Estevez J, , 2008. First visceral leishmaniasis focus in Argentina. Mem Inst Oswaldo Cruz 103: 109111.[Crossref]
  5. Basombrío MA, Taranto NJ, , 2000. Outbreaks of mucocutaneous leishmaniasis in northern Argentina. Anim Biol 9: 131133.
  6. Marco JD, Barroso P, Calvopiña M, Kumazawa H, Furuya M, Korenaga M, Cajal SP, Mora MC, Rea MM, Borda CE, Basombrío MA, Taranto NJ, Hashiguchi Y, , 2005. Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in northern Argentina. Am J Trop Med Hyg 72: 606611.
  7. Marco JD, Padilla AM, Diosque P, Fernandez MM, Malchiodi E, Basombrío MA, , 2001. Force of infection an evolution of lesions of canine tegumentary leishmaniasis in northwestern Argentina. Mem Inst Oswaldo Cruz 96: 649652.[Crossref]
  8. Padilla AM, Marco JD, Diosque P, Segura MA, Mora MC, Fernandez MM, Malchiodi EL, Basombrío MA, , 2002. Canine infection and the possible role of dogs in the transmission of American tegumentary leishmaniasis in Salta, Argentina. Vet Parasitol 110: 110.[Crossref]
  9. Barrouin-Melo SM, Farias-Laranjeira D, de Andrade-Filho FA, Trigo J, Juliao FS, Franke CR, Palis-Aguiar PH, Conrado-dos-Santos WL, Pontes-de-Carvalho L, , 2006. Can spleen aspirates be safely used for the parasitological diagnosis of canine visceral leishmaniasis? A study of asymptomatic and polysymptomatic animals. Vet J 171: 331339.[Crossref]
  10. Lainson R, Rangel EF, , 2005. Lutzomyia longipalpis and the eco-epidemiology of American visceral leishmaniasis, with particular reference to Brazil: a review. Mem Inst Oswaldo Cruz 100: 811827.[Crossref]
  11. Bravo AG, Quintana MG, Abril M, Salomón OD, , 2013. The first record of Lutzomyia longipalpis in the Argentine northwest. Mem Inst Oswaldo Cruz 108: 10711073.[Crossref]
  12. Barrio A, Parodi CM, Locatelli F, Mora MA, Basombrio MA, Korenaga M, Hashiguchi Y, García Bustos MF, Gentile A, Marco JD, , 2012. Leishmania infantum and visceral leishmaniasis, Argentina. Emerg Infect Dis 18: 354355.[Crossref]
  13. National Research Council Committee for the Update of the Guide for the Care and Use of Laboratory Animals, 2010. Guide for the Care and Use of Laboratory Animals, 8th edition. Washington, DC: National Academies Press, 248. Available at: http://www.nap.edu/catalog/12910.html.
  14. Marco JD, Barroso PA, Mimori T, Locatelli FM, Tomatani A, Mora MC, Cajal SP, Nasser JR, Parada LA, Taniguchi T, Korenaga M, Basombrío MA, Hashiguchi Y, , 2012. Polymorphism-specific PCR enhances the diagnostic performance of American tegumentary leishmaniasis and allows the rapid identification of Leishmania species from Argentina. BMC Infect Dis 12: 191198.[Crossref]
  15. Higuchi H, , 1990. Rapid, efficient DNA extraction for PCR from cells or blood. Perkin Elmer Cetus Amplifications 2: 13.
  16. Kato H, Uezato H, Katakura K, Calvopiña M, Marco JD, Barroso P, Gomez EA, Mimori T, Korenaga M, Iwata H, Nonaka S, Hashiguchi Y, , 2005. Detection and identification of Leishmania species within naturally infected sandflies in the Andean areas of Ecuador by polymerase chain reaction. Am J Trop Med Hyg 72: 8793.
  17. Young DG, Duncan MA, , 1994. Guide to the identification and geographic distribution of Lutzomyia sand flies in Mexico, the West Indies, Central and South America (Diptera: Psychodidae). Mem Am Entomol Inst 54: 1881.
  18. Tomasini N, Lauthier JJ, Llewellyn MS, Diosque P, , 2013. MLSTest: novel software for multi-locus sequence data analysis in eukaryotic organisms. Infect Genet Evol 20: 188196.[Crossref]
  19. Marco JD, Uezato H, Mimori T, Barroso PA, Korenaga M, Nonaka S, Basombrío MA, Taranto NJ, Hashiguchi Y, , 2006. Are cytochrome B sequencing and polymorphism-specific polymerase-chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? Am J Trop Med Hyg 75: 256260.
  20. Quinnell RJ, Carson C, Reithinger R, Garcez LM, Courtenay O, , 2009. Evaluation of rK39 rapid diagnostic tests for canine visceral leishmaniasis: longitudinal study and meta-analysis. PLoS Negl Trop Dis 7: e1992.[Crossref]
  21. Asato Y, Oshiro M, Myint CK, Yamamoto Y, Kato H, Marco JD, Mimori T, Gomez EA, Hashiguchi Y, Uezato H, , 2009. Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing. Exp Parasitol 121: 352361.[Crossref]
  22. Foulet F, Botterel F, Buffet P, Morizot G, Rivollet D, Deniau M, Pratlong F, Costa JM, Bretagne S, , 2007. Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome b gene. J Clin Microbiol 45: 21102115.[Crossref]
  23. Chaves LF, Hernandez MJ, Dobson AP, Pascual M, , 2007. Sources and sinks: revisiting the criteria for identifying reservoirs for American cutaneous leishmaniasis. Trends Parasitol 23: 311316.[Crossref]
  24. Silva ES, Gontijo CM, Melo MN, , 2005. Contribution of molecular techniques to the epidemiology of neotropical Leishmania species. Trends Parasitol 21: 550552.[Crossref]
  25. Barrio A, Mora MC, Ramos F, Moreno R, Samson R, Basombrío MA, , 2007. Short report: use of kDNA-based polymerase-chain reaction as sensitive and differentially diagnostic method of American tegumentary leishmaniasis in disease-endemic areas of northern Argentina. Am J Trop Med Hyg 77: 636639.
  26. Santini MS, Fernandez MS, Perez AA, Sandoval AE, Salomon OD, , 2012. Lutzomyia longipalpis abundance in the city of Posadas, north-east Argentina: variations at different spatial scales. Mem Inst Oswaldo Cruz 107: 767771.[Crossref]
  27. Salomón OD, Quintana MG, Bezzic G, Moranc ML, Bethbederc E, Valdez DV, , 2010. Short communication: Lutzomyia migonei as putative vector of visceral leishmaniasis in La Banda, Argentina. Acta Trop 113: 8487.[Crossref]
  28. Rosa J, Pereira DP, Brazil RP, Filho JD, Salomón O, Szelag E, , 2012. Natural infection of cortelezzii complex (Diptera: Psychodidae: Phlebotominae) with Leishmania braziliensis in Chaco, Argentina. Acta Trop 123: 128131.[Crossref]
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  • Received : 30 Apr 2014
  • Accepted : 10 Mar 2015

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