1921
Volume 91, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We evaluated the Newton Nm1, a commercially available handheld light microscope and compared it with conventional light microscopy for the diagnosis of soil-transmitted helminth infections. A total of 91 Kato-Katz thick smears were examined by experienced microscopists and helminth eggs were counted and expressed as eggs per gram of stool (EPG). Mean egg counts were significantly higher with the conventional light microscope (5,190 EPG versus 2,386 EPG for ; 826 versus 456 for ; both < 0.05). Using regression coefficients and accounting for intensity of infection, we found that the agreement between the two devices was excellent for both species (κ = 0.90, 95% confidence interval = 0.82–0.99 for and κ = 0.96, 95% CI = 0.91–1.00 for ). The Newton Nm1 microscope may be a useful tool for the detection and quantification of soil-transmitted helminth infection in clinical, epidemiologic, and public health settings.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.14-0253
2014-12-03
2017-11-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/91/6/1138.html?itemId=/content/journals/10.4269/ajtmh.14-0253&mimeType=html&fmt=ahah

References

  1. Pullan RL, Smith JL, Jasrasaria R, Brooker SJ, , 2014. Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasit Vectors 7: 37.[Crossref]
  2. Hotez PJ, Brindley PJ, Bethony JM, King CH, Pearce EJ, Jacobson J, , 2008. Helminth infections: the great neglected tropical diseases. J Clin Invest 118: 13111321.[Crossref]
  3. Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, Hotez PJ, , 2006. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. Lancet 367: 15211532.[Crossref]
  4. Hall A, Hewitt G, Tuffrey V, de Silva N, , 2008. A review and meta-analysis of the impact of intestinal worms on child growth and nutrition. Matern Child Nutr 4 (Suppl 1): 118236.[Crossref]
  5. Bergquist R, Johansen MV, Utzinger J, , 2009. Diagnostic dilemmas in helminthology: what tools to use and when? Trends Parasitol 25: 151156.[Crossref]
  6. Katz N, Chaves A, Pellegrino J, , 1972. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397400.
  7. Knopp S, Mgeni AF, Khamis IS, Steinmann P, Stothard JR, Rollinson D, Marti H, Utzinger J, , 2008. Diagnosis of soil-transmitted helminths in the era of preventive chemotherapy: effect of multiple stool sampling and use of different diagnostic techniques. PLoS Negl Trop Dis 2: e331.[Crossref]
  8. Speich B, Knopp S, Mohammed KA, Khamis IS, Rinaldi L, Cringoli G, Rollinson D, Utzinger J, , 2010. Comparative cost assessment of the Kato-Katz and FLOTAC techniques for soil-transmitted helminth diagnosis in epidemiological surveys. Parasit Vectors 3: 71.[Crossref]
  9. Stothard JR, Kabatereine NB, Tukahebwa EM, Kazibwe F, Mathieson W, Webster JP, Fenwick A, , 2005. Field evaluation of the Meade Readiview handheld microscope for diagnosis of intestinal schistosomiasis in Ugandan school children. Am J Trop Med Hyg 73: 949955.
  10. Bogoch II, Andrews JR, Speich B, Utzinger J, Ame SM, Ali SM, Keiser J, , 2013. Mobile phone microscopy for the diagnosis of soil-transmitted helminth infections: a proof-of-concept study. Am J Trop Med Hyg 88: 626629.[Crossref]
  11. Martin LK, Beaver PC, , 1968. Evaluation of Kato thick-smear technique for quantitative diagnosis of helminth infections. Am J Trop Med Hyg 17: 382391.
  12. World Health Organization, 2002. Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. WHO Tech Rep Ser 912: 157.
  13. Balsam J, Ossandon M, Bruck HA, Lubensky I, Rasooly A, , 2013. Low-cost technologies for medical diagnostics in low-resource settings. Expert Opin Med Diagn 7: 243255.[Crossref]
  14. Bogoch II, Coulibaly JT, Andrews JA, Speich B, Keiser J, Stothard JR, N'Goran EK, Utzinger J, , 2014. Evaluation of portable microscopic devices for the diagnosis of Schistosoma and soil-transmitted helminth infection. Parasitology (in press). DOI:http://dx.doi.org/10.1017/S0031182014000432).
  15. Lustigman S, Prichard RK, Gazzinelli A, Grant WN, Boatin BA, McCarthy JS, Basáñez M-G, , 2012. A research agenda for helminth diseases of humans: the problem of helminthiases. PLoS Negl Trop Dis 6: e1582255.[Crossref]
  16. Stephenson LS, Latham MC, Adams EJ, Kinoti SN, Pertet A, , 1993. Physical fitness, growth and appetite of Kenyan school boys with hookworm, Trichuris trichiura and Ascaris lumbricoides infections are improved four months after a single dose of albendazole. J Nutr 123: 10361046.
  17. Yap P, Fürst T, Müller I, Kriemler S, Utzinger J, Steinmann P, , 2012. Determining soil-transmitted helminth infection status and physical fitness of school-aged children. J Vis Exp 66: e3966.
  18. Lee M, Yaglidere O, Ozcan A, , 2011. Field-portable reflection and transmission microscopy based on lensless holography. Biomed Opt Express 2: 27212730.[Crossref]
  19. Greenbaum A, Sikora U, Ozcan A, , 2012. Field-portable wide-field microscopy of dense samples using multi-height pixel super-resolution based lensfree imaging. Lab Chip 12: 12421245.[Crossref]
  20. Isikman SO, Greenbaum A, Lee M, Bishara W, Mudanyali O, Su T-W, Ozcan A, , 2012. Lensfree computational microscopy tools for cell and tissue imaging at the point-of-care and in low-resource settings. Anal Cell Pathol Amst 35: 229247.[Crossref]
  21. Linder E, Grote A, Varjo S, Linder N, Lebbad M, Lundin M, Diwan V, Hannuksela J, Lundin J, , 2013. On-chip imaging of Schistosoma haematobium eggs in urine for diagnosis by computer vision. PLoS Negl Trop Dis 7: e2547.[Crossref]
  22. Tuijn CJ, Hoefman BJ, van Beijma H, Oskam L, Chevrollier N, , 2011. Data and image transfer using mobile phones to strengthen microscopy-based diagnostic services in low and middle income country laboratories. PLoS One 6: e28348.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.14-0253
Loading
/content/journals/10.4269/ajtmh.14-0253
Loading

Data & Media loading...

  • Received : 24 Apr 2014
  • Accepted : 07 Aug 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error