1921
Volume 92, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Early diagnosis of dengue has been made easier in recent years owing to the advancement in diagnostic technologies. The rapid non-structural protein 1 (NS1) test strip is widely used in many developed and developing regions at risk of dengue. Despite the relatively high specificity of this test, we recently encountered two cases of false-positive dengue NS1 antigen in patients with underlying hematological malignancies. We reviewed the literature for causes of false-positive dengue NS1.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.14-0247
2015-02-04
2020-11-26
Loading full text...

Full text loading...

/deliver/fulltext/14761645/92/2/367.html?itemId=/content/journals/10.4269/ajtmh.14-0247&mimeType=html&fmt=ahah

References

  1. Simmons CP, Farrar JJ, Nguyen vV, Wills B, 2012. Dengue. N Engl J Med 366: 14231432.[Crossref]
    [Google Scholar]
  2. World Health Organization, 2009. Dengue: Guidelines for Treatment, Prevention and Control. Geneva: World Health Organization.
    [Google Scholar]
  3. Andries AC, Duong V, Ngan C, Ong S, Huy R, Sroin KK, Te V, Y B, Try PL, Buchy P, 2012. Field evaluation and impact on clinical management of a rapid diagnostic kit that detects dengue NS1, IgM and IgG. PLoS Negl Trop Dis 6: e1993.[Crossref]
    [Google Scholar]
  4. Gan VC, Tan KL, Lye DC, Pok KY, Mok SQ, Chua RC, Leo YS, Ng LC, 2014. Diagnosing dengue at point-of-care: utility of a rapid combined diagnostic kit in Singapore. PLoS ONE 9: e90037.[Crossref]
    [Google Scholar]
  5. Blacksell SD, Jarman RG, Bailey MS, Tanganuchitcharnchai A, Jenjaroen K, Gibbons RV, Paris DH, Premaratna R, de Silva HJ, Lalloo DG, Day NP, 2011. Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy. Clin Vaccine Immunol 18: 20952101.[Crossref]
    [Google Scholar]
  6. Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45: 935941.[Crossref]
    [Google Scholar]
  7. Santiago GA, Vergne E, Quiles Y, Cosme J, Vazquez J, Medina JF, Colón C, Margolis H, Muñoz-Jordán JL, 2013. Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. PLoS Negl Trop Dis 7: e2311.[Crossref]
    [Google Scholar]
  8. Singapore National Environmental Agency. Campaign Against Dengue. Available at: http://dengue.gov.sg/. Accessed March 4, 2014.
    [Google Scholar]
  9. Alere, 2014. SD Bioline Dengue Duo NS1 Ag + Ab Combo. Available at: http://www.alere.com/ww/en/product-details/sd-bioline-dengue-duo-ns1-ag—ab-combo.html. Accessed March 4, 2014.
    [Google Scholar]
  10. Phuong HL, Thai KT, Nga TT, Giao PT, Hung le Q, Binh TQ, Nam NV, Groen J, de Vries PJ, 2009. Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever. Diagn Microbiol Infect Dis 63: 372378.[Crossref]
    [Google Scholar]
  11. Wang SM, Sekaran SD, 2010. Evaluation of a commercial SD dengue virus NS1 antigen capture enzyme-linked immunosorbent assay kit for early diagnosis of dengue virus infection. J Clin Microbiol 48: 27932797.[Crossref]
    [Google Scholar]
  12. Pok KY, Lai YL, Ang J, Ng LC, 2010. Evaluation of nonstructural 1 antigen assays for the diagnosis and surveillance of dengue in Singapore. Vector Borne Zoonotic Dis 10: 10091016.[Crossref]
    [Google Scholar]
  13. Colombo TE, Vedovello D, Araki CS, Cogo-Moreira H, dos Santos IN, Reis AF, Costa FR, Cruz LE, Casagrande L, Regatieri LJ, Junior JF, Bronzoni RV, Schmidt DJ, Nogueira ML, 2013. Dengue-4 false negative results by Panbio® dengue early ELISA assay in Brazil. J Clin Virol 58: 710712.[Crossref]
    [Google Scholar]
  14. Felix AC, Romano CM, Centrone C de C, Rodrigues CL, Villas-Boas L, Araújo ES, de Matos AM, Carvalho KI, Martelli CM, Kallas EG, Pannuti CS, Levi JE, 2012. Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil in 2010. Clin Vaccine Immunol 19: 19721976.[Crossref]
    [Google Scholar]
  15. Chuansumrit A, Chaiyaratana W, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2008. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J 27: 4348.[Crossref]
    [Google Scholar]
  16. Fry SR, Meyer M, Semple MG, Simmons CP, Sekaran SD, Huang JX, McElnea C, Huang CY, Valks A, Young PR, Cooper MA, 2011. The diagnostic sensitivity of dengue rapid test assays is significantly enhanced by using a combined antigen and antibody testing approach. PLoS Negl Trop Dis 5: e1199.[Crossref]
    [Google Scholar]
  17. Rueda E, Mendez A, Gonzalez G, 2002. Haemophagocytic syndrome associated with dengue hemorrhagic fever. Biomedica 22: 160166.[Crossref]
    [Google Scholar]
  18. Ramanathan M, Duraisamy G, 1991. Haemophagocytosis in dengue haemorrhagic fever: a case report. Ann Acad Med Singapore 20: 803804.
    [Google Scholar]
  19. Muller DA, Young PR, 2013. The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic marker. Antiviral Res 98: 192208.[Crossref]
    [Google Scholar]
  20. Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B, 2010. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J 7: 361.[Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.14-0247
Loading
/content/journals/10.4269/ajtmh.14-0247
Loading

Data & Media loading...

  • Received : 21 Apr 2014
  • Accepted : 29 Sep 2014
  • Published online : 04 Feb 2015
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error