Volume 91, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A modified imprint method, Press-Imprint-Smear, was compared with histopathology for the diagnosis of cutaneous leishmaniasis. Amastigotes were seen in 69 (92%) of 75 individuals in one or both assays. The Press-Imprint-Smear was positive in 85.3%, and histopathology was positive in 44%. Press-Imprint-Smear is a rapid and relatively sensitive method for the diagnosis of cutaneous leishmaniasis.


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  1. Alvar J, Velez ID, Bern C, Herero M, Desjeux P, Cano J, Jannin J, den Boer M, WHO Leishmaniasis Control Team; , 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7: e35671.[Crossref] [Google Scholar]
  2. Desjeux P, , 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305318.[Crossref] [Google Scholar]
  3. World Health Organization, 2007. Report of the Fifth Consultative Meeting on Leishmania/HIV Coinfection. Available at: http://www.who.int/leishmaniasis/resources/Leishmaniasis_hiv_coinfection5.pdf. Accessed November 1, 2013. [Google Scholar]
  4. Ministério da Saúde do Brasil, 2013. Casos de Leishmaniose Tegumentar Americana. Brasil. Grandes Regiões e Unidades Federadas. 1990–2011. Available at: http://portal.saude.gov.br/portal/arquivos/pdf/2012_11_casos_de_lta_entre_1990_e_2011.pdf. Accessed November 1, 2013. [Google Scholar]
  5. Levitt J, Bernardo S, Talley Whang T, , 2013. How to perform a punch biopsy of the skin—videos in clinical medicine. N Engl J Med 369: e13.[Crossref] [Google Scholar]
  6. De Lorenzi D, Mandara MT, Tranquillo M, Baroni M, Gasparinetti N, Gandini G, Masserdotti C, Bonfanti U, Bertolini G, Vian P, Bernardini M, , 2006. Squash–prep cytology in the diagnosis of canine and feline nervous system lesions: a study of 42 cases. Vet Clin Pathol 35: 208214.[Crossref] [Google Scholar]
  7. Mitra S, Kumar M, Sharma V, Mukhopadhyay D, , 2010. Squash preparation: a reliable diagnostic tool in the intraoperative diagnosis of central nervous system tumors. J Cytol 27: 8185.[Crossref] [Google Scholar]
  8. Alves CF, Alves CF, Figueiredo MM, Souza CC, Machado-Coelho GL, Melo MN, Tafuri WL, Raso P, Soares RP, Tafuri WL, , 2013. American tegumentary leishmaniasis: effectiveness of an immunohistochemical protocol for the detection of Leishmania in skin. PLoS ONE 8: e63343.[Crossref] [Google Scholar]
  9. Berman JD, , 1997. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years. Clin Infect Dis 24: 684703.[Crossref] [Google Scholar]
  10. Weigle KA, de Dávalos M, Heredia P, Molineros R, Saravia NG, D'Alessandro A, , 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489496. [Google Scholar]
  11. Sousa AQ, Parise ME, Pompeu MM, Coelho Filho JM, Vasconcelos IA, Lima JW, Oliveira EG, Vasconcelos AW, David JR, Maguire JH, , 1995. Bubonic leishmaniasis: a common manifestation of Leishmania (Viannia) braziliensis infection in Ceara, Brazil. Am J Trop Med Hyg 53: 380385. [Google Scholar]
  12. Sousa AQ, Pompeu MML, Frutuoso MS, Moraes EA, Pearson RD, , 2012. Reply to Drs. Torres and Suarez. Clin Infect Dis 55: 309310.[Crossref] [Google Scholar]
  13. Aviles H, Belli A, Armijos R, Monroy FP, Harris E, , 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85: 181187.[Crossref] [Google Scholar]
  14. Ramirez JR, Agudelo S, Muskus C, , 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitological diagnosis. J Clin Microbiol 38: 37683773. [Google Scholar]
  15. Sharquie KE, Hassen AS, Hassan SA, Al-Hamami IA, , 2002. Evaluation of diagnosis of cutaneous leishmaniasis by direct smear, culture and histopathology. Saudi Med J 23: 925928. [Google Scholar]

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  • Received : 17 Mar 2014
  • Accepted : 07 Jul 2014
  • Published online : 05 Nov 2014

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