Volume 91, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A woman who recently traveled to Thailand came to a local emergency department with a fever and papular rash. She was tested for measles, malaria, and dengue. Positive finding for IgM antibody against dengue and a failure to seroconvert for IgG against dengue for multiple blood samples suggested an alternate flavivirus etiology. Amplification of a conserved region of the non-structural protein 5 gene of the genus yielded a polymerase chain reaction product with a matching sequence of 99% identity with Zika virus. A urine sample and a nasopharygeal swab specimen obtained for the measles investigation were also positive for this virus by reverse transcription polymerase chain reaction. Subsequently, the urine sample yielded a Zika virus isolate in cell culture. This case report describes a number of novel clinical and laboratory findings, the first documentation of this virus in Canada, and the second documentation from this region in Thailand.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Ayers M, Adachi D, Johnson G, Andonova M, Drebot M, Tellier R, , 2006. A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses. J Virol Methods 135: 235239.[Crossref] [Google Scholar]
  2. ProMed-mail. Zika Virus in a Returning Canadian Traveler. May 29, 2013. Available at: http://www.promed.org, archive no 20130529.1744108. [Google Scholar]
  3. Tappe D, Rissland J, Gabriel M, Emmerich P, Günther S, Held G, Smola S, Schmidt-Chanasit J, , 2014. First case of laboratory-confirmed Zika virus infection imported into Europe, November 2013. Euro Surveill 19: 20685.[Crossref] [Google Scholar]
  4. Dick GW, Kitchen SF, Haddow AJ, , 1952. Zika virus (I). Isolations and serological specificity. Trans R Soc Trop Med Hyg 46: 509520.[Crossref] [Google Scholar]
  5. Heang V, Yasuda CY, Sovann L, Haddow AD, Travassos da Rosa AP, Tesh RB, Kasper MR, , 2012. Zika virus infection, Cambodia, 2010. Emerg Infect Dis 18: 349350.[Crossref] [Google Scholar]
  6. Duffy MR, Chen TH, Hancock WT, Powers AM, Kool JL, Lanciotti RS, Pretrick M, Marfel M, Holzbauer S, Dubray C, Guillaumot L, Griggs A, Bel M, Lambert AJ, Laven J, Kosoy O, Panella A, Biggerstaff BJ, Fischer M, Hayes EB, , 2009. Zika virus outbreak on Yap Island, Federated States of Micronesia. N Engl J Med 360: 25362543.[Crossref] [Google Scholar]
  7. European Centre for Disease Prevention and Control (ECDC). Rapid Risk Assessment: Zika Virus Infection Outbreak, French Polynesia, February 14, 2014. Stockholm: ECDC; 2014. Available at: http://www.ecdc.europa.eu/en/publications/Publications/Zika-virus-French-Polynesia-rapid-risk-assessment.pdf. [Google Scholar]
  8. Rudolph KE, Lessler J, Moloney RM, Kmush B, Cummings DAT, , 2014. Incubation periods of mosquito-borne viral infections: a systematic review. Am J Trop Med Hyg 90: 882891.[Crossref] [Google Scholar]
  9. Foy BD, Kobylinski KC, Foy JLC, Blitvich BJ, Travassos da Rosa A, Haddow AD, Lanciotti RS, Tesh RB, , 2011. Probable non–vector-borne transmission of Zika virus, Colorado, USA. Emerg Infect Dis 17: 880882.[Crossref] [Google Scholar]
  10. Musso D, Nhan T, Robin E, Roche C, Bierlaire D, Zisou K, Shan Yan A, Cao-Lormeau VM, Broult J, , 2014. Potential for Zika virus transmission though blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014. Euro Surveill 19: pii: 20761.[Crossref] [Google Scholar]
  11. Petersen LR, Busch MP, , 2010. Transfusion-transmitted arboviruses. Vox Sang 98: 495503.[Crossref] [Google Scholar]
  12. Huhtamo E, Hasu E, Uzcategui NY, Erra E, Nikkari S, Kantele A, Vapalahti O, Piiparinen H, , 2010. Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology. J Clin Virol 47: 4953.[Crossref] [Google Scholar]
  13. Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, Stanfield SM, Duffy MR, , 2008. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis 14: 12321239.[Crossref] [Google Scholar]
  14. Kwong JC, Druce JD, Leder K, , 2013. Zika virus infection acquired during brief travel to Indonesia. Am J Trop Med Hyg 89: 516517.[Crossref] [Google Scholar]
  15. Lertanekawattana S, Anantapreecha S, Jiraphongsa C, Duan-ngern P, Potjalongsin S, Wiittayabamrung W, Daroon P, Techolarn M, , 2013. Prevalence and characteristics of dengue and chikungunya infections among acute febrile patients in Nong Khai Province, Thailand. Southeast Asian J Trop Med Public Health 44: 780790. [Google Scholar]
  16. Chen LH, Wilson ME, , 2010. Dengue and chikungunya infections in travelers. Curr Opin Infect Dis 23: 438444.[Crossref] [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, , 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony method. Mol Biol Evol 28: 27312739.[Crossref] [Google Scholar]

Data & Media loading...

  • Received : 13 Mar 2014
  • Accepted : 14 Jul 2014
  • Published online : 05 Nov 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error