Volume 92, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The aim of this study was to investigate the seroprevalence of antichikungunya virus (anti-CHIKV) antibodies in pregnant women living in an urban area of Benin (West Africa). Results were obtained by screening sera collected in 2006 and 2007 with enzyme-linked immunosorbent assay (ELISA) for anti-CHIKV immunoglobulin G (IgG) and IgM. Positive results were confirmed by indirect immunofluorescence test and microneutralization assay. We found that a large proportion (36.1%) of pregnant women living in Cotonou had specific IgG against CHIKV, indicating a high seroprevalence of the infection in urban southern Benin, whereas no active cases of CHIKV infection were detected.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, Hunsperger E, Kroeger A, Margolis HS, Martinez E, Nathan MB, Pelegrino JL, Simmons C, Yoksan S, Peeling RW, , 2010. Dengue: a continuing global threat. Nat Rev Microbiol 8: S7S16.[Crossref] [Google Scholar]
  2. Queyriaux B, Simon F, Grandadam M, Michel R, Tolou H, Boutin JP, , 2008. Clinical burden of chikungunya virus infection. Lancet Infect Dis 8: 23.[Crossref] [Google Scholar]
  3. Charrel RN, de Lamballerie X, Raoult D, , 2007. Chikungunya outbreaks–the globalization of vectorborne diseases. N Engl J Med 356: 769771.[Crossref] [Google Scholar]
  4. Thonnon J, Spiegel A, Diallo M, Diallo A, Fontenille D, , 1999. Chikungunya virus outbreak in Senegal in 1996 and 1997. Bull Soc Pathol Exot 92: 7982. [Google Scholar]
  5. Pastorino B, Muyembe-Tamfum JJ, Bessaud M, Tock F, Tolou H, Durand JP, Peyrefitte CN, , 2004. Epidemic resurgence of chikungunya virus in Democratic Republic of the Congo: identification of a new central African strain. J Med Virol 74: 277282.[Crossref] [Google Scholar]
  6. Pialoux G, Gauzere BA, Jaureguiberry S, Strobel M, , 2007. Chikungunya, an epidemic arbovirosis. Lancet Infect Dis 7: 319327.[Crossref] [Google Scholar]
  7. Gould LH, Osman MS, Farnon EC, Griffith KS, Godsey MS, Karch S, Mulenda B, El Kholy A, Grandesso F, de Radigues X, Brair ME, Briand S, El Tayeb el SM, Hayes EB, Zeller H, Perea W, , 2008. An outbreak of yellow fever with concurrent chikungunya virus transmission in South Kordofan, Sudan, 2005. Trans R Soc Trop Med Hyg 102: 12471254.[Crossref] [Google Scholar]
  8. Hertz JT, Munishi OM, Ooi EE, Howe S, Lim WY, Chow A, Morrissey AB, Bartlett JA, Onyango JJ, Maro VP, Kinabo GD, Saganda W, Gubler DJ, Crump JA, , 2012. Chikungunya and dengue fever among hospitalized febrile patients in northern Tanzania. Am J Trop Med Hyg 86: 171177.[Crossref] [Google Scholar]
  9. Caron M, Paupy C, Grard G, Becquart P, Mombo I, Nso BB, Kassa F, Nkoghe D, Leroy EM, , 2012. Recent introduction and rapid dissemination of chikungunya virus and dengue virus serotype 2 associated with human and mosquito coinfections in Gabon, central Africa. Clin Infect Dis 55: e45e53.[Crossref] [Google Scholar]
  10. Kelvin AA, , 2011. Outbreak of Chikungunya in the Republic of Congo and the global picture. J Infect Dev Ctries 5: 441444. [Google Scholar]
  11. Sutherland LJ, Cash AA, Huang YJ, Sang RC, Malhotra I, Moormann AM, King CL, Weaver SC, King CH, LaBeaud AD, , 2011. Serologic evidence of arboviral infections among humans in Kenya. Am J Trop Med Hyg 85: 158161.[Crossref] [Google Scholar]
  12. Kuniholm MH, Wolfe ND, Huang CY, Mpoudi-Ngole E, Tamoufe U, LeBreton M, Burke DS, Gubler DJ, , 2006. Seroprevalence and distribution of Flaviviridae, Togaviridae, and Bunyaviridae arboviral infections in rural Cameroonian adults. Am J Trop Med Hyg 74: 10781083. [Google Scholar]
  13. Mease LE, Coldren RL, Musila LA, Prosser T, Ogolla F, Ofula VO, Schoepp RJ, Rossi CA, Adungo N, , 2011. Seroprevalence and distribution of arboviral infections among rural Kenyan adults: a cross-sectional study. Virol J 8: 371.[Crossref] [Google Scholar]
  14. Pistone T, Ezzedine K, Boisvert M, Receveur MC, Schuffenecker I, Zeller H, Lafon ME, Fleury H, Malvy D, , 2009. Cluster of chikungunya virus infection in travelers returning from Senegal, 2006. J Travel Med 16: 286288.[Crossref] [Google Scholar]
  15. Jentes ES, Robinson J, Johnson BW, Conde I, Sakouvougui Y, Iverson J, Beecher S, Bah MA, Diakite F, Coulibaly M, Bausch DG, Bryan J, , 2010. Acute arboviral infections in Guinea, West Africa, 2006. Am J Trop Med Hyg 83: 388394.[Crossref] [Google Scholar]
  16. Ansumana R, Jacobsen KH, Leski TA, Covington AL, Bangura U, Hodges MH, Lin B, Bockarie AS, Lamin JM, Bockarie MJ, Stenger DA, , 2013. Reemergence of chikungunya virus in Bo, Sierra Leone. Emerg Infect Dis 19: 11081110.[Crossref] [Google Scholar]
  17. Baba M, Logue CH, Oderinde B, Abdulmaleek H, Williams J, Lewis J, Laws TR, Hewson R, Marcello A, D'Agaro P, , 2013. Evidence of arbovirus co-infection in suspected febrile malaria and typhoid patients in Nigeria. J Infect Dev Ctries 7: 5159.[Crossref] [Google Scholar]
  18. Fievet N, Varani S, Ibitokou S, Briand V, Louis S, Perrin RX, Massougbogji A, Hosmalin A, Troye-Blomberg M, Deloron P, , 2009. Plasmodium falciparum exposure in utero, maternal age and parity influence the innate activation of foetal antigen presenting cells. Malar J 8: 251.[Crossref] [Google Scholar]
  19. Nahum A, Erhart A, Mayé A, Ahounou D, van Overmeir C, Menten J, van Loen H, Akogbeto M, Coosemans M, Massougbodji A, D'Alessandro U, , 2010. Malaria incidence and prevalence among children living in a peri-urban area on the coast of Benin, West Africa: a longitudinal study. Am J Trop Med Hyg 83: 465473.[Crossref] [Google Scholar]
  20. Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli AC, Panning M, Cordioli P, Fortuna C, Boros S, Magurano F, Silvi G, Angelini P, Dottori M, Ciufolini MG, Majori GC, Cassone A, , 2007. Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet 370: 18401846.[Crossref] [Google Scholar]
  21. Eisenhut M, Schwarz TF, Hegenscheid B, , 1999. Seroprevalence of dengue, chikungunya and sindbis virus infections in German aid workers. Infection 27: 8285.[Crossref] [Google Scholar]
  22. Fritel X, Rollot O, Gerardin P, Gauzere BA, Bideault J, Lagarde L, Dhuime B, Orvain E, Cuillier F, Ramful D, Samperiz S, Jaffar-Bandjee MC, Michault A, Cotte L, Kaminski M, Fourmaintraux A, , 2006. Chikungunya virus infection during pregnancy, Reunion, France. Emerg Infect Dis 16: 418425.[Crossref] [Google Scholar]
  23. Gérardin P, Barau G, Michault A, Bintner M, Randrianaivo H, Choker G, Lenglet Y, Touret Y, Bouveret A, Grivard P, Le Roux K, Blanc S, Schuffenecker I, Couderc T, Arenzana-Seisdedos F, Lecuit M, Robillard PY, , 2008. Multidisciplinary prospective study of mother-to-child chikungunya virus infections on the island of La Reunion. PLoS Med 5: e60.[Crossref] [Google Scholar]
  24. Ramful D, Carbonnier M, Pasquet M, Bouhmani B, Ghazouani J, Noormahomed T, Beullier G, Attali T, Samperiz S, Fourmaintraux A, Alessandri JL, , 2007. Mother-to-child transmission of Chikungunya virus infection. Pediatr Infect Dis J 26: 811815.[Crossref] [Google Scholar]
  25. Dauby N, Goetghebuer T, Kollmann TR, Levy J, Marchant A, , 2012. Uninfected but not unaffected: chronic maternal infections during pregnancy, fetal immunity, and susceptibility to postnatal infections. Lancet Infect Dis 12: 330340.[Crossref] [Google Scholar]

Data & Media loading...

  • Received : 10 Feb 2014
  • Accepted : 13 Nov 2014
  • Published online : 03 Jun 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error