Volume 91, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Studies investigating winter transmission of Eastern equine encephalitis virus (EEEV) were conducted in Hillsborough County, Florida. The virus was detected in and in February 2012 and 2013, respectively. During the winter months, herons were the most important avian hosts for all mosquito species encountered. In collections carried out in the summer of 2011, blood meals taken from herons were still common, but less frequently encountered than in winter, with an increased frequency of mammalian- and reptile-derived meals observed in the summer. Four wading bird species (Black-crowned Night Heron [], Yellow-crowned Night Heron [], Anhinga [], and Great Blue Heron []) were most frequently fed upon by and , suggesting that these species may participate in maintaining EEEV during the winter in Florida.


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  1. Scott TW, Weaver SC, , 1989. Eastern equine encephalomyelitis virus: epidemiology and evolution of mosquito transmission. Adv Virus Res 37: 277328.[Crossref] [Google Scholar]
  2. Villari P, Spielman A, Komar N, McDowell M, Timperi RJ, , 1995. The economic burden imposed by a residual case of eastern encephalitis. Am J Trop Med Hyg 52: 813. [Google Scholar]
  3. US Geological Service, 2014. ArboNET. Available at: https://wwwn.cdc.gov/arbonet/. Accessed January 5, 2014.
  4. Crans WJ, Caccamise DF, McNelly JR, , 1994. Eastern equine encephalomyelitis virus in relation to the avian community of a coastal cedar swamp. J Med Entomol 31: 711728.[Crossref] [Google Scholar]
  5. Chamberlain RW, Sikes RK, Nelson DB, Sudia WD, , 1954. Studies on the North American arthropod-borne encephalitides. VI. Quantitative determinations of virus-vector relationships. Am J Hyg 60: 278285. [Google Scholar]
  6. Burkett-Cadena ND, McClure CJ, Ligon RA, Graham SP, Guyer C, Hill GE, Ditchkoff SS, Eubanks MD, Hassan HK, Unnasch TR, , 2011. Host reproductive phenology drives seasonal patterns of host use in mosquitoes. PLoS ONE 6: e17681.[Crossref] [Google Scholar]
  7. Kilpatrick AM, Kramer LD, Jones MJ, Marra PP, Daszak P, , 2006. West Nile virus epidemics in North America are driven by shifts in mosquito feeding behavior. PLoS Biol 4: e82.[Crossref] [Google Scholar]
  8. Molaei G, Andreadis TG, Armstrong PM, Anderson JF, Vossbrinck CR, , 2006. Host feeding patterns of Culex mosquitoes and West Nile virus transmission, northeastern United States. Emerg Infect Dis 12: 468474.[Crossref] [Google Scholar]
  9. Cupp EW, Klingler K, Hassan HK, Viguers LM, Unnasch TR, , 2003. Transmission of eastern equine encephalomyelitis virus in central Alabama. Am J Trop Med Hyg 68: 495500. [Google Scholar]
  10. Cohen SB, Lewoczko K, Huddleston DB, Moody E, Mukherjee S, Dunn JR, Jones TF, Wilson R, Moncayo AC, , 2009. Host feeding patterns of potential vectors of eastern equine encephalitis virus at an epizootic focus in Tennessee. Am J Trop Med Hyg 81: 452456. [Google Scholar]
  11. Burkett-Cadena ND, White GS, Eubanks MD, Unnasch TR, , 2011. Winter biology of wetland mosquitoes at a focus of eastern equine encephalomyelitis virus transmission in Alabama, USA. J Med Entomol 48: 967973.[Crossref] [Google Scholar]
  12. Kissling RE, Chamberlain RW, Nelson DB, Stamm DD, , 1955. Studies on the North American arthropod-borne encephalitides. VIII. Equine encephalitis studies in Louisiana. Am J Hyg 62: 233254. [Google Scholar]
  13. Hassan HK, Cupp EW, Hill GE, Katholi CR, Klingler K, Unnasch TR, , 2003. Avian host preference by vectors of eastern equine encephalomyelitis virus. Am J Trop Med Hyg 69: 641647. [Google Scholar]
  14. Estep LK, McClure CJ, Burkett-Cadena ND, Hassan HK, Hicks TL, Unnasch TR, Hill GE, , 2011. A multi-year study of mosquito feeding patterns on avian hosts in a southeastern focus of eastern equine encephalitis virus. Am J Trop Med Hyg 84: 718726.[Crossref] [Google Scholar]
  15. Stamm DD, , 1958. Studies on the ecology of equine encephalomyelitis. Am J Pub Health Nat Health 48: 328335.[Crossref] [Google Scholar]
  16. Spalding MG, McLean RG, Burgess JH, Kirk LJ, , 1994. Arboviruses in water birds (Ciconiiformes, Pelecaniformes) from Florida. J Wildl Dis 30: 216221.[Crossref] [Google Scholar]
  17. Herman CM, , 1962. The role of birds in the epizootiology of eastern equine encephalitis. Auk 79: 99103.[Crossref] [Google Scholar]
  18. Aguirre AA, McLean RG, Cook RS, , 1992. Experimental inoculation of three arboviruses in black-bellied whistling ducks (Dendrocygna autumnalis). J Wildl Dis 28: 521525.[Crossref] [Google Scholar]
  19. McLean RG, Crans WJ, Caccamise DF, McNelly J, Kirk LJ, Mitchell CJ, Calisher CH, , 1995. Experimental infection of wading birds with eastern equine encephalitis virus. J Wildl Dis 31: 502508.[Crossref] [Google Scholar]
  20. Reisen WK, Wheeler S, Armijos MV, Fang Y, Garcia S, Kelley K, Wright S, , 2009. Role of communally nesting ardeid birds in the epidemiology of West Nile virus revisited. Vector Borne Zoonotic Dis 9: 275280.[Crossref] [Google Scholar]
  21. Powers CD, Dickerman RW, , 1975. Primary immunoglobulin response of herons to infection with Venezuelan encephalitis virus. Infect Immun 11: 303308. [Google Scholar]
  22. Scherer WF, Smith RP, , 1960. In vitro studies on the sites of Japanese encephalitis virus multiplication in the heron, an important natural host in Japan. Am J Trop Med Hyg 9: 5055. [Google Scholar]
  23. Boyle DB, Dickerman RW, Marshall ID, , 1983. Primary viremia responses of herons to experimental infection with Murray Valley encephalitis, Kunjin and Japanese encephalitis viruses. Aust J Exp Biol Med Sci 61: 655664.[Crossref] [Google Scholar]
  24. Bigler WJ, Lassing EB, Buff EE, Prather EC, Beck EC, Hoff GL, , 1976. Endemic eastern equine encephalomyelitis in Florida: a twenty-year analysis, 1955–1974. Am J Trop Med Hyg 25: 884890. [Google Scholar]
  25. Armstrong PM, Andreadis TG, Anderson JF, Stull JW, Mores CN, , 2008. Tracking eastern equine encephalitis virus perpetuation in the northeastern United States by phylogenetic analysis. Am J Trop Med Hyg 79: 291296. [Google Scholar]
  26. White GS, Pickett BE, Lefkowitz EJ, Johnson AG, Ottendorfer C, Stark LM, Unnasch TR, , 2011. Phylogenetic analysis of eastern equine encephalitis virus isolates from Florida. Am J Trop Med Hyg 84: 709717.[Crossref] [Google Scholar]
  27. Young DS, Kramer LD, Maffei JG, Dusek RJ, Backenson PB, Mores CN, Bernard KA, Ebel GD, , 2008. Molecular epidemiology of eastern equine encephalitis virus, New York. Emerg Infect Dis 14: 454460.[Crossref] [Google Scholar]
  28. Burkett-Cadena ND, , 2011. A wire-frame shelter for collecting resting mosquitoes. J Am Mosq Control Assoc 27: 152155.[Crossref] [Google Scholar]
  29. Burkett-Cadena ND, Eubanks MD, Unnasch TR, , 2008. Preference of female mosquitoes for natural and artificial resting sites. J Am Mosq Control Assoc 24: 228235.[Crossref] [Google Scholar]
  30. Darsie RF, Ward RA, , 2005. Identification and Geographical Distribution of the Mosquitoes of North America, North of Mexico. Gainesville, FL: University of Florida Press, 383 pp. [Google Scholar]
  31. Lambert AJ, Martin DA, Lanciotti RS, , 2003. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J Clin Microbiol 41: 379385.[Crossref] [Google Scholar]
  32. Volchkov VE, Volchkova VA, Netesov SV, , 1991. Complete nucleotide sequence of the Eastern equine encephalomyelitis virus genome. Mol Gen Mikrobiol Virusol 5: 815. [Google Scholar]
  33. Florida Department of Health, 2011. Real-time RT-PCR Arbovirology Protocol–2011. Tallahassee, FL: Florida Department of Health Bureau of Laboratories. [Google Scholar]
  34. Burkett-Cadena ND, Graham SP, Hassan HK, Guyer C, Eubanks MD, Katholi CR, Unnasch TR, , 2008. Blood feeding patterns of potential arbovirus vectors of the genus kCulex targeting ectothermic hosts. Am J Trop Med Hyg 79: 809815. [Google Scholar]
  35. Kitano T, Umetsu K, Tian W, Osawa M, , 2007. Two universal primer sets for species identification among vertebrates. Int J Legal Med 121: 423427.[Crossref] [Google Scholar]
  36. Butcher GS, Fuller MR, McAllister LS, Geissler P, , 1990. An evaluation of the Christmas bird count for monitoring population trends of selected species. Wildl Soc Bull 18: 129134. [Google Scholar]
  37. Butcher GS, Niven DK, Sauer JR, , 2005. Using Christmas Bird Count data to assess population dynamics and trends of waterbirds. Am Birds 59: 2325. [Google Scholar]
  38. McCrimmon DA, Fryska ST, Ogden JC, Butcher GS, , 1997. Nonlinear population dynamics of six species of Florida Ciconiiformes assessed by Christmas Bird Counts. Ecol Appl 7: 581592.[Crossref] [Google Scholar]
  39. Tozer D, Abraham K, Nol E, , 2006. Improving the accuracy of counts of wetland breeding birds at the point scale. Wetlands 26: 518527.[Crossref] [Google Scholar]
  40. Nadeau CP, Conway CJ, Smith BS, Lewis TE, , 2008. Maximizing detection probability of wetland-dependent birds during point-count surveys in northwestern Florida. Wilson J Ornithol 120: 513518.[Crossref] [Google Scholar]
  41. Colwell MA, Cooper RJ, , 1993. Estimates of coastal shorebird abundance: the important of multiple counts. J Field Ornithol 64: 293301. [Google Scholar]
  42. Apperson CS, Hassan HK, Harrison BA, Savage HM, Aspen SE, Farajollahi A, Crans W, Daniels TJ, Falco RC, Benedict M, Anderson M, McMillen L, Unnasch TR, , 2004. Host feeding patterns of established and potential mosquito vectors of West Nile virus in the eastern United States. Vector Borne Zoonotic Dis 4: 7182.[Crossref] [Google Scholar]
  43. Johnson DH, Gibbs JP, Herzog M, Lor S, Niemuth ND, Ribic CA, Seamans M, Shaffer TL, Shriver WG, Stehman SV, Thompson WL, , 2009. A sampling design framework for monitoring secretive marshbirds. Waterbirds 32: 203215.[Crossref] [Google Scholar]
  44. Day JF, Stark LM, , 1996. Eastern equine encephalitis transmission to emus (Dromaius novaehollandiae) in Volusia County, Florida: 1992 through 1994. J Am Mosq Control Assoc 12: 429436. [Google Scholar]
  45. Edman JD, Webber LA, Kale HW, 2nd, 1972. Host-feeding patterns of Florida mosquitoes. II. Culiseta . J Med Entomol 9: 429434.[Crossref] [Google Scholar]
  46. Edman JD, , 1979. Host-feeding patterns of Florida mosquitoes (Diptera: Culicidae). VI. Culex (Melanoconion). J Med Entomol 15: 521525.[Crossref] [Google Scholar]
  47. Nichols J, Thomas L, Conn P, Thomson D, Cooch E, Conroy M, , 2009. Inferences about landbird abundance from count data: Recent advances and future directions. , eds. Modeling Demographic Processes in Marked Populations. New York, NY: Springer, 201235.[Crossref] [Google Scholar]
  48. Fletcher RJ, Hutto RL, Haukos DA, , 2006. Estimating detection probabilities of river birds using double surveys. Auk 123: 695707.[Crossref] [Google Scholar]
  49. Webber LA, Edman JD, , 1972. Anti-mosquito behavior of ciconiiform birds. Anim Behav 20: 228232.[Crossref] [Google Scholar]
  50. Oliveira A, Katholi CR, Burkett-Cadena N, Hassan HK, Kristensen S, Unnasch TR, , 2011. Temporal analysis of feeding patterns of Culex erraticus in central Alabama. Vector Borne Zoonotic Dis 11: 413421.[Crossref] [Google Scholar]

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  • Received : 06 Feb 2014
  • Accepted : 27 Jun 2014
  • Published online : 01 Oct 2014

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