Volume 93, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In August 2013, Xishuangbanna, Yunnan Province, China, had its first dengue outbreak. Dengue virus (DENV) RNA detection in sera or viral isolates revealed that all 222 autochthonous patients detected and three Chinese travelers from Laos (imported cases) were positive for DENV-3 serotype, while DENV-1 and DENV-4 were detected in travelers from Myanmar and Thailand during the outbreak. For 33 suspected dengue cases collected before the outbreak, two imported cases from Laos and nine residents living in Laos (Laotian cases) were positive for DENV-3. Further, a random subset of 33 positive cases for DENV-3 was sequenced for the full envelope gene of DENV. Phylogenetic analysis showed that all of the 25 autochthonous cases sequenced were grouped into the same clade, genotype II of DENV-3, with imported cases from Laos and Laotian cases. These results suggest that the genotype II of DENV-3 was associated with the outbreak and may have originated from the virus circulating in Laos.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Article metrics loading...

Loading full text...

Full text loading...



  1. Wang E, Ni H, Xu R, Barrett AD, Watowich SJ, Gubler DJ, Weaver SC, , 2000. Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. J Virol 74: 32273234.[Crossref]
  2. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI, , 2013. The global distribution and burden of dengue. Nature 496: 504507.[Crossref]
  3. Fan WF, Yu SR, Cosgriff TM, , 1981. The reemergence of dengue in China. Rev Infect Dis 4: S847S853.
  4. Wu JY, Lun ZR, James AA, Chen XG, , 2010. Dengue fever in mainland China. Am J Trop Med Hyg 83: 664671.[Crossref]
  5. Guo XF, Wu C, Wang PY, Mao XH, Zhou HN, Yang J, Dong SH, Ling YX, Gao SQ, , 2010. Serological investigation of dengue in western Yunnan. Chin J Zoonoses 26: 502507.
  6. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV, , 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545551.
  7. Liang H, Luo L, Yang Z, Di B, Bai Z, He P, Jing Q, Zheng X, , 2013. Re-emergence of dengue virus type 3 in Canton, China, 2009–2010, associated with multiple introductions through different geographical routes. PLoS One 8: e55353.[Crossref]
  8. Huang JH, Su CL, Yang CF, Liao TL, Hsu TC, Chang SF, Lin CC, Shu PY, , 2012. Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008–2010. Am J Trop Med Hyg 87: 349358.[Crossref]
  9. Lanciotti RS, Lewis JG, Gubler DJ, Trent DW, , 1994. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75: 6575.[Crossref]
  10. Guo XF, Zhao QM, Wu C, Zuo SQ, Zhang XA, Jia N, Liu JY, Zhou HN, Zhang JS, , 2013. First isolation of dengue virus from Lao PDR in a Chinese traveler. Virol J 10: 70.[Crossref]

Data & Media loading...

Supplementary Data

Supplementary PDF

  • Received : 19 Jan 2014
  • Accepted : 02 May 2015

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error