Volume 91, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



spp. isolated from patients during a multiyear outbreak in Thailand were genotyped using multilocus sequence typing and a majority were identified as ST34, especially in earlier years. We tested whether ST34 isolates were better adapted to survive in various pH levels, temperatures, and water sources. Motility and growth were monitored over a 12-week period. Early year ST34 isolates did not appear to have a significant fitness advantage over non-ST34, however, this may have been because a majority of the isolates survived to the termination of the study, with the exception being at high temperature (37°C) and/or basic pH (8.65). Failure to detect a significant fitness advantage of ST34 may be a result of the length of the study or the small sample size. Lengthening the study and looking at virulence and maintenance in the host could yield additional information about this outbreak.


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  1. Levett PN, , 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref] [Google Scholar]
  2. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, , 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757771.[Crossref] [Google Scholar]
  3. Thaipadungpanit J, Wuthiekanun V, Chierakul W, Smythe LD, Petkanchanapong W, Limpaiboon R, Apiwatanaporn A, Slack AT, Suputtamongkol Y, White NJ, Feil EJ, Day NP, Peacock SJ, , 2007. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Negl Trop Dis 1: e56.[Crossref] [Google Scholar]
  4. Tangkanakul W, Smits HL, Jatanasen S, Ashford DA, , 2005. Leptospirosis: an emerging health problem in Thailand. Southeast Asian J Trop Med Public Health 36: 281288. [Google Scholar]
  5. Johnson RC, Harris VG, , 1967. Differentiation of pathogenic and saprophytic letospires. I. Growth at low temperatures. J Bacteriol 94: 2731. [Google Scholar]
  6. Turner LH, , 1970. Leptospirosis. 3. Maintenance, isolation and demonstration of leptospires. Trans R Soc Trop Med Hyg 64: 623646.[Crossref] [Google Scholar]
  7. Trueba G, Zapata S, Madrid K, Cullen P, Haake D, , 2004. Cell aggregation: a mechanism of pathogenic Leptospira to survive in fresh water. Int Microbiol 7: 3540. [Google Scholar]
  8. Goris MG, Hartskeerl RA, , 2014. Leptospirosis serodiagnosis by the microscopic agglutination test. Curr Protoc Microbiol 32: 12E 5 112E 5 18.[Crossref] [Google Scholar]
  9. Cole JR, Jr Sulzer CR, Pursell AR, , 1973. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol 25: 976980. [Google Scholar]
  10. Ellinghausen HC, Jr, 1973. Growth temperatures, virulence, survival, and nutrition of leptospires. J Med Microbiol 6: 487497.[Crossref] [Google Scholar]
  11. Schreier S, Triampo W, Doungchawee G, Triampo D, Chadsuthi S, , 2009. Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires. Biol Res 42: 512.[Crossref] [Google Scholar]
  12. Stevens JP, , 2009. Applied Multivariate Statistics for the Social Sciences. Fifth edition. Mahwah, NJ: Lawrence Erlbaum Associates. [Google Scholar]
  13. Klein JP, Moeschberger ML, , 2003. Survival Analysis: Techniques for Censored and Truncated Data. Second edition. New York: Springer. [Google Scholar]
  14. Westfall PH, Tobias RD, Rom D, Wolfinger RD, Hochberg Y, , 1999. Multiple Comparisons and Multiple Tests Using the SAS System. Cary, NC: SAS Institute. [Google Scholar]
  15. Chang SL, Buckingham M, Taylor MP, , 1948. Studies on Leptospira icterohaemorrhagiae; survival in water and sewage; destruction in water by halogen compounds, synthetic detergents, and heat. J Infect Dis 82: 256266.[Crossref] [Google Scholar]
  16. Kirschner L, Maguire T, , 1957. Survival of Leptospira outside their hosts. N Z Med J 56: 385391. [Google Scholar]
  17. Okazaki W, Ringen LM, , 1957. Some effects of various environmental conditions on the survival of Leptospira pomona . Am J Vet Res 18: 219223. [Google Scholar]
  18. Smith CE, Turner LH, , 1961. The effect of pH on the survival of leptospires in water. Bull World Health Organ 24: 3543. [Google Scholar]
  19. Parker J, Walker M, , 2011. Survival of a pathogenic Leptospira serovar in response to combined in vitro pH and temperature stresses. Vet Microbiol 152: 146150.[Crossref] [Google Scholar]

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  • Received : 17 Dec 2013
  • Accepted : 17 Jul 2014
  • Published online : 05 Nov 2014

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