Volume 91, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



This study evaluated rickettsial infection in 701 fleas that were collected from dogs and cats in 31 municipalities, encompassing all regions and major biomes of Brazil. A total of 268 (38.2%) fleas from 30 municipalities were polymerase chain reaction (PCR) positive for the rickettsial gene. The PCR products from 44 fleas, consisting of at least 1 PCR-positive flea from each of 30 municipalities, generated DNA sequences identical to . Rickettsial prevalence was highly variable among 30 municipalities, with values ranging from 2.9% to 100%. Significantly higher infection rates by were associated with the Pampa biome (southern Brazil), and the temperate climate that prevails in southern Brazil. In contrast, lowest -infection rates were significantly associated with the Caatinga biome, and its semiarid climate. Further studies should evaluate the effect of temperature and moisture on the infection in fleas world widely.


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  1. Adams JR, Schmidtmann ET, Azad AF, , 1990. Infection of colonized cat fleas, Ctenocephalides felis (Bouché), with a Rickettsia-like microorganism. Am J Trop Med Hyg 43: 400409.
  2. Bouyer DH, Stenos J, Crocquet-Valdes P, Moron CG, Popov VL, Zavala-Velazquez JE, Foil LD, Stothard DR, Azad AF, Walker DH, , 2001. Rickettsia felis: molecular characterization of a new member of the spotted fever group. Int J Syst Evol Microbiol 51: 339347.[Crossref]
  3. Choi YJ, Lee EM, Park JM, Lee KM, Han SH, Kim JK, Lee SH, Song HJ, Choi MS, Kim IS, Park KH, Jang WJ, , 2007. Molecular detection of various rickettsiae in mites (Acari: Trombiculidae) in southern Jeolla Province, Korea. Microbiol Immunol 51: 307312.[Crossref]
  4. Tsui PY, Tsai KH, Weng MH, Hung YW, Liu YT, Hu KY, Lien JC, Lin PR, Shaio MF, Wang HC, Ji DD, , 2007. Molecular detection and characterization of spotted fever group rickettsiae in Taiwan. Am J Trop Med Hyg 77: 883890.
  5. Thepparit C, Sunyakumthorn P, Guillotte ML, Popov VL, Foil LD, Macaluso KR, , 2011. Isolation of a rickettsial pathogen from a non-hematophagous arthropod. PLoS ONE 6: e16396.[Crossref]
  6. Abarca K, López J, Acosta-Jamett G, Martínez-Valdebenito C, , 2013. Rickettsia felis in Rhipicephalus sanguineus from two distant Chilean cities. Vector Borne Zoonotic Dis 13: 607609.[Crossref]
  7. Socolovschi C, Pagés F, Raoult D, , 2012. Rickettsia felis in Aedes albopictus mosquitoes, Libreville, Gabon. Emerg Infect Dis 18: 16871689.[Crossref]
  8. Socolovschi C, Pagés F, Ndiath MO, Ratmanov P, Raoult D, , 2012. Rickettsia species in African Anopheles mosquitoes. PLoS ONE 7: e48254.[Crossref]
  9. Pérez-Osorio CE, Zavala-Velazquez JE, Arias Leon JJ, Zavala-Castro JE, , 2008. Rickettsia felis as emergent global threat for humans. Emerg Infect Dis 14: 10191023.[Crossref]
  10. Reif KE, Macaluso KR, , 2009. Ecology of Rickettsia felis: a review. J Med Entomol 46: 723736.[Crossref]
  11. Mediannikov O, Cabre O, Qu F, Socolovschi C, Davoust B, Marié J, Parola P, Raoult D, , 2011. Rickettsia felis and Bartonella clarridgeiae in fleas from New Caledonia. Vector Borne Zoonotic Dis 11: 181183.[Crossref]
  12. Troyo A, Aìlvare D, Taylor L, Abdalla G, Calderon-Arguedas O, Zambrano ML, Dasch GA, Lindblade K, Hun L, Eremeeva ME, Esteìvez A, , 2012. Rickettsia felis in Ctenocephalides felis from Guatemala and Costa Rica. Am J Trop Med Hyg 86: 10541056.[Crossref]
  13. Oliveira RP, Galvao MA, Mafra CL, Chamone CB, Calic SB, Silva SU, Walker DH, , 2002. Rickettsia felis in Ctenocephalides spp. fleas, Brazil. Emerg Infect Dis 8: 317319.[Crossref]
  14. Horta MC, Labruna MB, Pinter A, Linardi PM, Schumaker TT, , 2007. Rickettsia infection in five areas of the State of São Paulo, Brazil. Mem Inst Oswaldo Cruz 102: 793801.[Crossref]
  15. Gehrke FS, Gazeta GS, Souza ER, Ribeiro A, Marrelli MT, Schumaker TT, , 2009. Rickettsia rickettsii, Rickettsia felis and Rickettsia sp. TwKM03 infecting Rhipicephalus sanguineus and Ctenocephalides felis collected from dogs in a Brazilian spotted fever focus in the State of Rio De Janeiro/Brazil. Clin Microbiol Infect S2: 267268.[Crossref]
  16. Raoult D, La Scola B, Enea M, Fournier PE, Roux V, Fenollar F, Galvao MA, Lamballerie X, , 2001. A flea-associated Rickettsia pathogenic for humans. Emerg Infect Dis 7: 7381.[Crossref]
  17. Maina AN, Knobel DL, Jiang J, Halliday J, Feikin DR, Cleaveland S, Nganga Z, Junghae M, Breiman RF, Richards AL, Njenga MK, , 2012. Infection in febrile patients, western Kenya, 2007–2010. Emerg Infect Dis 18: 328331.[Crossref]
  18. Nilsson K, Wallménius K, Hartwig S, Norlander T, Pahlson C, , 2013. Bell's palsy and sudden deafness associated with Rickettsia spp. Infection in Sweden. A retrospective and prospective serological survey including PCR findings. Eur J Neurol 21: 206214.[Crossref]
  19. Linardi PM, Guimarães LR, , 2000. Sifonápteros do Brasil. São Paulo: Museu de Zoologia USP/FAPESP.
  20. Labruna MB, Whitworth T, Horta MC, Bouyer DH, McBride JW, Pinter A, Popov V, Gennari SM, Walker DH, , 2004. Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of Sao Paulo, Brazil, where Brazilian spotted fever is endemic. J Clin Microbiol 42: 9098.[Crossref]
  21. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, , 1990. Basic local alignment search tool. J Mol Biol 215: 403410.[Crossref]
  22. Junk WJ, Cunha CN, Wantzen KM, Petermann P, Strussmann C, Marques MI, Adis J, , 2006. Biodiversity and its conservation in the Pantanal of Mato Grosso, Brazil. Aquat Sci 68: 278309.[Crossref]
  23. Mendonça F, Danni-Oliveira IM, , 2007. Climatologia: noções básicas e climas do Brasil. São Paulo: Oficina de Textos.
  24. Parola P, , 2011. Rickettsia felis: from a rare disease in the USA to a common cause of fever in sub-Saharan Africa. Clin Microbiol Infect 17: 9961000.[Crossref]
  25. Mediannikov O, Abdissa A, Diatta G, Trape J-F, Raoult D, , 2012. Rickettsia felis in fleas, southern Ethiopia. Emerg Infect Dis 18: 13851386.[Crossref]
  26. Katz G, Camargo Neves VL, Angerami RN, Nascimento EM, Colombo S, , 2009. Situação epidemiológica e importância da febre maculosa brasileira no Estado de São Paulo. Boletim Epidemiológico Paulista 6: 413.
  27. Del Fiol FS, Junqueira FM, Rocha MC, Toledo MI, Filho SB, , 2010. Rocky Mountain spotted fever in Brazil. Rev Panam Salud Publica 27: 461466.[Crossref]
  28. Horta MC, Scott FB, Correia TR, Fernandes JI, Richtzenhain LJ, Labruna MB, , 2010. Rickettsia felis infection in cat fleas Ctenocephalides felis felis . Braz J Microbiol 41: 813818.[Crossref]

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  • Received : 01 Dec 2013
  • Accepted : 25 Mar 2014

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