1921
Volume 90, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Leptospirosis is a zoonotic disease that causes severe manifestations such as Weil's disease and pulmonary hemorrhage syndrome. The aim of this study was to evaluate whether reactive oxygen species (ROS) production and antioxidant reduced glutathione (GSH) levels are related to complications in patients hospitalized with leptospirosis. The ROS production and GSH levels were measured in blood samples of 12 patients and nine healthy controls using chemiluminescence and absorbance assays. We found that ROS production was higher and GSH levels were lower in leptospirosis patients compared with healthy individuals. Among patients, GSH depletion was correlated with thrombocytopenia and elevated serum creatinine, whereas a strong positive correlation was observed between ROS production and elevated serum potassium. Additional investigation of the biological significance of ROS production and GSH levels is warranted as they may guide the development of novel adjuvant therapies for leptospirosis targeting oxidative stress.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.13-0667
2014-04-02
2017-09-22
Loading full text...

Full text loading...

/deliver/fulltext/14761645/90/4/719.html?itemId=/content/journals/10.4269/ajtmh.13-0667&mimeType=html&fmt=ahah

References

  1. Ko AI, Goarant C, Picardeau M, , 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7: 736747.[Crossref]
  2. McBride AJ, Athanazio DA, Reis MG, Ko AI, , 2005. Leptospirosis. Curr Opin Infect Dis 18: 376386.[Crossref]
  3. Medeiros FR, Spichler A, Athanazio DA, , 2010. Leptospirosis-associated disturbances of blood vessels, lungs and hemostasis. Acta Trop 115: 155162.[Crossref]
  4. WHO, 2003. Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Malta: World Health Organization.
  5. Gouveia EL, Metcalfe J, de Carvalho AL, Aires TS, Villasboas-Bisneto JC, Queirroz A, Santos AC, Salgado K, Reis MG, Ko AI, , 2008. Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. Emerg Infect Dis 14: 505508.[Crossref]
  6. Cerqueira TB, Athanazio DA, Spichler AS, Seguro AC, , 2008. Renal involvement in leptospirosis–new insights into pathophysiology and treatment. Braz J Infect Dis 12: 248252.[Crossref]
  7. Medeiros F da R, Spichler A, Athanazio DA, , 2010. Leptospirosis-associated disturbances of blood vessels, lungs and hemostasis. Acta Trop 115: 155162.[Crossref]
  8. Yang CW, Hung CC, Wu MS, Tian YC, Chang CT, Pan MJ, Vandewalle A, , 2006. Toll-like receptor 2 mediates early inflammation by leptospiral outer membrane proteins in proximal tubule cells. Kidney Int 69: 815822.[Crossref]
  9. Yang CW, Wu MS, Pan MJ, Hong JJ, Yu CC, Vandewalle A, Huang CC, , 2000. Leptospira outer membrane protein activates NF-kappaB and downstream genes expressed in medullary thick ascending limb cells. J Am Soc Nephrol 11: 20172026.
  10. Yang CW, Wu MS, Pan MJ, Hsieh WJ, Vandewalle A, Huang CC, , 2002. The Leptospira outer membrane protein LipL32 induces tubulointerstitial nephritis-mediated gene expression in mouse proximal tubule cells. J Am Soc Nephrol 13: 20372045.[Crossref]
  11. Maciel EA, Athanazio DA, Reis EA, Cunha FQ, Queiroz A, Almeida D, McBride AJ, Ko AI, Reis MG, , 2006. High serum nitric oxide levels in patients with severe leptospirosis. Acta Trop 100: 256260.[Crossref]
  12. Spichler A, Ko AI, Silva EF, De Brito T, Silva AM, Athanazio D, Silva C, Seguro A, , 2007. Reversal of renal tubule transporter downregulation during severe leptospirosis with antimicrobial therapy. Am J Trop Med Hyg 77: 11111119.
  13. Goode HF, Cowley HC, Walker BE, Howdle PD, Webster NR, , 1995. Decreased antioxidant status and increased lipid peroxidation in patients with septic shock and secondary organ dysfunction. Crit Care Med 23: 646651.[Crossref]
  14. Galley HF, Davies MJ, Webster NR, , 1996. Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. Crit Care Med 24: 16491653.[Crossref]
  15. Cowley HC, Bacon PJ, Goode HF, Webster NR, Jones JG, Menon DK, , 1996. Plasma antioxidant potential in severe sepsis: a comparison of survivors and nonsurvivors. Crit Care Med 24: 11791183.[Crossref]
  16. Chuang CC, Shiesh SC, Chi CH, Tu YF, Hor LI, Shieh CC, Chen MF, , 2006. Serum total antioxidant capacity reflects severity of illness in patients with severe sepsis. Crit Care 10: R36.[Crossref]
  17. Borrelli E, Roux-Lombard P, Grau GE, Girardin E, Ricou B, Dayer J, Suter PM, , 1996. Plasma concentrations of cytokines, their soluble receptors, and antioxidant vitamins can predict the development of multiple organ failure in patients at risk. Crit Care Med 24: 392397.[Crossref]
  18. Cross CE, Forte T, Stocker R, Louie S, Yamamoto Y, Ames BN, Frei B, , 1990. Oxidative stress and abnormal cholesterol metabolism in patients with adult respiratory distress syndrome. J Lab Clin Med 115: 396404.
  19. Wagenaar JF, Goris MG, Gasem MH, Isbandrio B, Moalli F, Mantovani A, Boer KR, Hartskeerl RA, Garlanda C, van Gorp EC, , 2009. Long pentraxin PTX3 is associated with mortality and disease severity in severe leptospirosis. J Infect 58: 425432.[Crossref]
  20. Siriwanij T, Suttinont C, Tantawichien T, Chusil S, Kanjanabuch T, Sitprija V, , 2005. Hemodynamics in leptospirosis: effects of plasmapheresis and continuous venovenous hemofiltration. Nephrology (Carlton) 10: 16.[Crossref]
  21. Ko AI, Galvao Reis M, Ribeiro Dourado CM, Johnson WD, Jr Riley LW, , 1999. Urban epidemic of severe leptospirosis in Brazil. Salvador Leptospirosis Study Group. Lancet 354: 820825.[Crossref]
  22. Mashimo M, Nishikawa M, Higuchi K, Hirose M, Wei Q, Haque A, Sasaki E, Shiba M, Tominaga K, Watanabe T, Fujiwara Y, Arakawa T, Inoue M, , 2006. Production of reactive oxygen species in peripheral blood is increased in individuals with Helicobacter pylori infection and decreased after its eradication. Helicobacter 11: 266271.[Crossref]
  23. Noronha-Dutra AA, Epperlein MM, Woolf N, , 1993. Reaction of nitric oxide with hydrogen peroxide to produce potentially cytotoxic singlet oxygen as a model for nitric oxide-mediated killing. FEBS Lett 321: 5962.[Crossref]
  24. Erdogan HM, Karapehlivan M, Citil M, Atakisi O, Uzlu E, Unver A, , 2008. Serum sialic acid and oxidative stress parameters changes in cattle with leptospirosis. Vet Res Commun 32: 333339.[Crossref]
  25. Mishra V, , 2007. Oxidative stress and role of antioxidant supplementation in critical illness. Clin Lab 53: 199209.
  26. Rinaldi S, Landucci F, De Gaudio AR, , 2009. Antioxidant therapy in critically septic patients. Curr Drug Targets 10: 872880.[Crossref]
  27. Victor VM, Espulgues JV, Hernandez-Mijares A, Rocha M, , 2009. Oxidative stress and mitochondrial dysfunction in sepsis: a potential therapy with mitochondria-targeted antioxidants. Infect Disord Drug Targets 9: 376389.[Crossref]
  28. Campos R, Shimizu MH, Volpini RA, de Braganca AC, Andrade L, Lopes FD, Olivo C, Canale D, Seguro AC, , 2012. N-acetylcysteine prevents pulmonary edema and acute kidney injury in rats with sepsis submitted to mechanical ventilation. Am J Physiol Lung Cell Mol Physiol 302: L640L650.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.13-0667
Loading
/content/journals/10.4269/ajtmh.13-0667
Loading

Data & Media loading...

  • Received : 16 Nov 2013
  • Accepted : 27 Dec 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error