1921
Volume 90, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.

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References

  1. Foss NT, Motta ACF, , 2012. Leprosy, a neglected disease that causes a wide variety of clinical conditions in tropical countries. Mem Inst Oswaldo Cruz, Rio de Janeiro 107 (Suppl 1): 2833.[Crossref] [Google Scholar]
  2. Shepard CC, McRae DH, , 1968. A method for counting acid-fast bacteria. Int J Lepr 36: 7882. [Google Scholar]
  3. Buhrer-Sekula S, Smits HL, Gussenhoven GC, van Leeuwen J, Amador S, Fujiwara T, Klatser PR, Oskam L, , 2003. Simple and fast lateral flow test for classification of leprosy patients and identification of contacts with high risk of developing leprosy. J Clin Microbiol 41: 19911995.[Crossref] [Google Scholar]
  4. Douglas JT, Steven LM, Fajardo T, Cellona RV, Madarang MG, Abalos RM, Steenbergen GJ, , 1988. The effects of chemotherapy on antibody levels in lepromatous patients. Lepr Rev 59: 127135. [Google Scholar]
  5. Santos VS, de Mendonça Neto PT, Falcão Raposo OF, Fakhouri R, Reis FP, Feitosa VL, , 2013. Evaluation of agreement between clinical and histopathological data for classifying leprosy. Int J Infect Dis 17: e189e192.[Crossref] [Google Scholar]
  6. Clark-Curtiss JE, Docherty MA, , 1989. A species-specific repetitive sequence in Mycobacterium leprae DNA. J Infect Dis 159: 715.[Crossref] [Google Scholar]
  7. Katoch VM, , 2001. Newer diagnostic techniques for tuberculosis. Indian J Med Res 120: 418428. [Google Scholar]
  8. Hampson SJ, Portaels F, Thompson J, Green EP, Moss MT, Hermon-Taylor J, McFadden JJ, , 1989. DNA probes demonstrate a single highly conserved strain of Mycobacterium avium infecting AIDS patients. Lancet 1: 6568.[Crossref] [Google Scholar]
  9. Yoon D-H, Cho S-N, Lee MK, Abalos RM, Cellona RV, Fajardo TT, Jr Guido LS, Dela Cruz EC, Walsh GP, Kim JD, , 1993. Evaluation of polymerase chain reaction amplification of Mycobacterium leprae-specific repetitive sequence in biopsy specimens from leprosy patients. J Clin Microbiol 31: 895899. [Google Scholar]
  10. Martinez AN, Ribeiro-Alves M, Sarno EN, Moraes MO, , 2011. Evaluation of qPCR-based assays for leprosy diagnosis directly in clinical specimens. PLoS Negl Trop Dis 5: e1354.[Crossref] [Google Scholar]
  11. Kramme S, Bretzel G, Panning M, Kawuma J, Drosten C, , 2004. Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR. Med Microbiol Immunol (Berl) 193: 189193.[Crossref] [Google Scholar]
  12. Truman RW, Andrews PK, Robbins NY, Adams LB, Krahenbuhl JL, Gillis TP, , 2008. Enumeration of Mycobacterium leprae using real-time PCR. PLoS Negl Trop Dis 2: e328.[Crossref] [Google Scholar]
  13. Hardman WJ, Benian GM, Howard T, McGowan JE, Jr Metchock B, Murtagh JJ, , 1996. Rapid detection of mycobacteria in inflammatory necrotizing granulomas from formalin fixed, paraffin-embedded tissue by PCR in clinically high-risk patients with acid-fast stain and culture-negative tissue biopsies. Am J Clin Pathol 106: 384389.[Crossref] [Google Scholar]
  14. Wen Y, Xing Y, Yuan LC, Liu J, Zhang Y, Li HY, , 2013. Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy. Am J Trop Med Hyg 88: 918922.[Crossref] [Google Scholar]
  15. Ridley DS, Jopling WH, , 1966. Classification of leprosy according to immunity – a five group system. Int J Lepr 34: 255273. [Google Scholar]
  16. Lockwood DN, Nicholls P, Smith WC, Das L, Barkataki P, van Brakel W, Suneetha S, , 2012. Comparing the clinical and histological diagnosis of leprosy and leprosy reactions in the INFIR cohort of Indian patients with multibacillary leprosy. PLoS Negl Trop Dis 6: e1702.[Crossref] [Google Scholar]
  17. Cole ST, Supply P, Honoré N, , 2001. Repetitive sequences in Mycobacterium leprae and their impact on genome plasticity. Lepr Rev 72: 449461. [Google Scholar]
  18. Rodrigues LC, Lockwood DN, , 2011. Leprosy now: epidemiology, progress, challenges, and research gaps. Lancet Infect Dis 11: 464470.[Crossref] [Google Scholar]
  19. Lini N, Shankernarayan NP, Dharmalingam K, , 2009. Quantitative real-time PCR analysis of Mycobacterium leprae DNA and mRNA in human biopsy material from leprosy and reactional cases. J Med Microbiol 58: 753759.[Crossref] [Google Scholar]
  20. Scollard DM, Adams LB, Gillis TP, Kranenbuthl JL, Truman RW, Williams DL, , 2006. The continuing challenges of leprosy. Clin Microbiol Rev 19: 338381.[Crossref] [Google Scholar]
  21. Bhatia AS, Katoch K, Narayanan RB, Ramu G, Mukherjee A, Lavania RK, , 1993. Clinical and histopathological correlation in the classification of leprosy. Int J Lepr 61: 433438. [Google Scholar]
  22. Martinez AN, Britto CF, Nery JA, Sampaio EP, Jardim MR, Sarno EN, Moraes MO, , 2006. Evaluation of real-time and conventional PCR targeting complex 85 genes for detection of Mycobacterium leprae DNA in skin biopsy samples from patients diagnosed with leprosy. J Clin Microbiol 44: 31543159.[Crossref] [Google Scholar]
  23. Patrocínio LG, Goulart IM, Goulart LR, Patrocínio JA, Ferreira FR, Fleury RN, , 2005. Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction. FEMS Immunol Med Microbiol 44: 311316.[Crossref] [Google Scholar]
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  • Received : 12 Nov 2013
  • Accepted : 23 Dec 2013
  • Published online : 05 Mar 2014

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