1921
Volume 90, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of rHsp83 compared with -like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA–-like when considering either CL/ML ( = 0.041) or all leishmaniasis patients ( = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA–-like, specifically for Chagas disease samples ( < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML ( = 0.607) or CL ( = 0.205). We recommend ELISA–-rHsp83 as a routine confirmatory serological assay for the diagnosis of infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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2014-05-07
2017-09-21
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References

  1. Pan American Health Organization, 2013. Leishmaniases. Report Leishmaniases 1: 14.
  2. Goto H, Prianti M, , 2009. Immunoactivation and immunopathogeny during active visceral leishmaniasis. Rev Inst Med Trop Sao Paulo 51: 241246.[Crossref]
  3. Goto H, Lauletta Lindoso JA, , 2012. Cutaneous and mucocutaneous leishmaniasis. Infect Dis Clin North Am 26: 293307.[Crossref]
  4. Goto H, Lindoso JA, , 2010. Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis. Expert Rev Anti Infect Ther 8: 419433.[Crossref]
  5. Al-Hucheimi SN, Sultan BA, Al-Dhalimi MA, , 2009. A comparative study of the diagnosis of Old World cutaneous leishmaniasis in Iraq by polymerase chain reaction and microbiologic and histopathologic methods. Int J Dermatol 48: 404408.[Crossref]
  6. Schubach A, Cuzzi-Maya T, Oliveira AV, Sartori A, de Oliveira-Neto MP, Mattos MS, Araujo ML, Souza WJ, Haddad F, Perez M de A, Pacheco RS, Momen H, Coutinho SG, de Almeida Marzochi MC, Marzochi KB, da Costa SC, , 2001. Leishmanial antigens in the diagnosis of active lesions and ancient scars of American tegumentary leishmaniasis patients. Mem Inst Oswaldo Cruz 96: 987996.[Crossref]
  7. Sotto MN, Yamashiro-Kanashiro EH, da Matta VL, de Brito T, , 1989. Cutaneous leishmaniasis of the New World: diagnostic immunopathology and antigen pathways in skin and mucosa. Acta Trop 46: 121130.[Crossref]
  8. Vega-Lopez F, , 2003. Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 16: 97101.[Crossref]
  9. Romero GA, Boelaert M, , 2010. Control of visceral leishmaniasis in Latin America—a systematic review. PLoS Negl Trop Dis 4: e584.[Crossref]
  10. Srividya G, Kulshrestha A, Singh R, Salotra P, , 2012. Diagnosis of visceral leishmaniasis: developments over the last decade. Parasitol Res 110: 10651078.[Crossref]
  11. Edrissian GH, Darabian P, , 1979. A comparison of enzyme-linked immunosorbent assay and indirect fluorescent antibody test in the sero-diagnosis of cutaneous and visceral leishmaniasis in Iran. Trans R Soc Trop Med Hyg 73: 289292.[Crossref]
  12. Kar K, , 1995. Serodiagnosis of leishmaniasis. Crit Rev Microbiol 21: 123152.[Crossref]
  13. Barroso-Freitas AP, Passos SR, Mouta-Confort E, Madeira MF, Schubach AO, Santos GP, Nascimento LD, Marzochi MC, Marzochi KB, , 2009. Accuracy of an ELISA and indirect immunofluorescence for the laboratory diagnosis of American tegumentary leishmaniasis. Trans R Soc Trop Med Hyg 103: 383389.[Crossref]
  14. Guimaraes MC, Celeste BJ, Franco EL, Cuce LC, Belda W, Jr, 1989. Evaluation of serological diagnostic indices for mucocutaneous leishmaniasis: immunofluorescence tests and enzyme-linked immunoassays for IgG, IgM and IgA antibodies. Bull World Health Organ 67: 643648.
  15. el Safi SH, Evans DA, , 1989. A comparison of the direct agglutination test and enzyme-linked immunosorbent assay in the sero-diagnosis of leishmaniasis in the Sudan. Trans R Soc Trop Med Hyg 83: 334337.[Crossref]
  16. Guimaraes MC, Celeste BJ, Franco EL, , 1990. Diagnostic performance indices for immunofluorescent tests and enzyme immunoassays of leishmaniasis sera from northern and north-eastern Brazil. Bull World Health Organ 68: 3943.
  17. Zeyrek FY, Korkmaz M, Ozbel Y, , 2007. Serodiagnosis of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica in Sanliurfa Province, Turkey, where ACL Is highly endemic. Clin Vaccine Immunol 14: 14091415.[Crossref]
  18. Romero GA, de la Gloria Orge Orge M, de Farias Guerra MV, Paes MG, de Oliveira Macedo V, de Carvalho EM, , 2005. Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil. Acta Trop 93: 4956.[Crossref]
  19. Srivastava P, Dayama A, Mehrotra S, Sundar S, , 2011. Diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg 105: 16.[Crossref]
  20. Cunningham J, Hasker E, Das P, El Safi S, Goto H, Mondal D, Mbuchi M, Mukhtar M, Rabello A, Rijal S, Sundar S, Wasunna M, Adams E, Menten J, Peeling R, Boelaert M, WHO/TDR Visceral Leishmaniasis Laboratory Network; , 2012. A global comparative evaluation of commercial immunochromatographic rapid diagnostic tests for visceral leishmaniasis. Clin Infect Dis 55: 13121319.[Crossref]
  21. Rey-Ladino JA, Joshi PB, Singh B, Gupta R, Reiner NE, , 1997. Leishmania major: molecular cloning, sequencing, and expression of the heat shock protein 60 gene reveals unique carboxy terminal peptide sequences. Exp Parasitol 85: 249263.[Crossref]
  22. Amorim AG, Carrington M, Miles MA, Barker DC, de Almeida ML, , 1996. Identification of the C-terminal region of 70 kDa heat shock protein from Leishmania (Viannia) braziliensis as a target for the humoral immune response. Cell Stress Chaperones 1: 177187.[Crossref]
  23. Souza AP, Soto M, Costa JM, Boaventura VS, de Oliveira CI, Cristal JR, Barral-Netto M, Barral A, , 2013. Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins. PLoS ONE 8: e66110.[Crossref]
  24. Celeste BJ, Angel SO, Castro LG, Gidlund M, Goto H, , 2004. Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis. Braz J Med Biol Res 37: 15911593.[Crossref]
  25. Angel SO, Requena JM, Soto M, Criado D, Alonso C, , 1996. During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response. Acta Trop 62: 4556.[Crossref]
  26. Momen H, Grimaldi G, Jr Pacheco RS, Jaffe CL, McMahon-Pratt D, Marzochi MC, , 1985. Brazilian Leishmania stocks phenotypically similar to Leishmania major . Am J Trop Med Hyg 34: 10761084.
  27. Hoshino-Shimizu S, Camargo ME, Nagasse TK, , 1978. A stable polysaccharide-hemagglutination reagent for the diagnosis of acute or recent Trypanosoma cruzi infections. Rev Inst Med Trop Sao Paulo 20: 208212.
  28. Guimaraes MC, Celeste BJ, de Castilho EA, Mineo JR, Diniz JM, , 1981. Immunoenzymatic assy (ELISA) in mucocutaneous leishmaniasis, kala-azar, and Chagas' disease: an epimastigote Trypanosoma cruzi antigen able to distinguish between anti-Trypanosoma and anti-Leishmania antibodies. Am J Trop Med Hyg 30: 942947.
  29. de Arruda MM, Figueiredo FB, Cardoso FA, Hiamamoto RM, Brazuna JC, de Oliveira MR, Noronha EF, Romero GA, , 2013. Validity and reliability of enzyme immunoassays using Leishmania major or L. infantum antigens for the diagnosis of canine visceral leishmaniasis in Brazil. PLoS ONE 8: e69988.[Crossref]
  30. Guimaraes MC, Celeste BJ, Corrales EM, Antunes CM, , 1991. Comparison on the performance of Leishmania major-like and Leishmania braziliensis braziliensis as antigen for New World leishmaniasis IgG-immunofluorescence test. Rev Inst Med Trop Sao Paulo 33: 503508.[Crossref]
  31. Cuba Cuba CA, Marsden PD, Barreto AC, Rocha R, Sampaio RR, Patzlaff L, , 1981. Parasitologic and immunologic diagnosis of American (mucocutaneous) leishmaniasis. Bull Pan Am Health Organ 15: 249259.
  32. Montenegro J, , 1926. A cutis-reação na leishmaniose. An Fac Med Univ Sao Paulo 1: 323330.
  33. Greiner M, Pfeiffer D, Smith RD, , 2000. Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests. Prev Vet Med 45: 2341.[Crossref]
  34. Chappuis F, Rijal S, Soto A, Menten J, Boelaert M, , 2006. A meta-analysis of the diagnostic performance of the direct agglutination test and rK39 dipstick for visceral leishmaniasis. BMJ 333: 723.[Crossref]
  35. Weigle KA, de Davalos M, Heredia P, Molineros R, Saravia NG, D'Alessandro A, , 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489496.
  36. Marsden PD, , 1986. Mucosal leishmaniasis (“espundia” Escomel, 1911). Trans R Soc Trop Med Hyg 80: 859876.[Crossref]
  37. Pedras MJ, de Gouvea Viana L, de Oliveira EJ, Rabello A, , 2008. Comparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg 102: 172178.[Crossref]
  38. Mohapatra TM, Singh DP, Sen MR, Bharti K, Sundar S, , 2010. Compararative evaluation of rK9, rK26 and rK39 antigens in the serodiagnosis of Indian visceral leishmaniasis. J Infect Dev Ctries 4: 114117.[Crossref]
  39. Houghton RL, Petrescu M, Benson DR, Skeiky YA, Scalone A, Badaro R, Reed SG, Gradoni L, , 1998. A cloned antigen (recombinant K39) of Leishmania chagasi diagnostic for visceral leishmaniasis in human immunodeficiency virus type 1 patients and a prognostic indicator for monitoring patients undergoing drug therapy. J Infect Dis 177: 13391344.[Crossref]
  40. Singh S, Sivakumar R, , 2003. Recent advances in the diagnosis of leishmaniasis. J Postgrad Med 49: 5560.[Crossref]
  41. Abramo C, Fontes CJ, Krettli AU, , 1995. Cross-reactivity between antibodies in the sera of individuals with leishmaniasis, toxoplasmosis, and Chagas' disease and antigens of the blood-stage forms of Plasmodium falciparum determined by indirect immunofluorescence. Am J Trop Med Hyg 53: 202205.
  42. de Oliveira MR, Macedo V de O, de Carvalho EM, Barral A, Marotti JG, Bittencourt A, de Abreu MV, Orge MLG, Lessa H de A, Marsden PD, , 1995. An evolutionary study of mucosal leishmaniasis (a 7- to 17-year follow-up) due to Leishmania (Viannia) braziliensis in Tres Bracos, Bahia. Rev Soc Bras Med Trop 28: 325332.[Crossref]
  43. Walton BC, , 1980. Evaluation of chemotherapy of American leishmaniasis by the indirect fluorescent antibody test. Am J Trop Med Hyg 29: 747752.
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  • Received : 25 Oct 2013
  • Accepted : 23 Jan 2014

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