Volume 90, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Routine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of rHsp83 compared with -like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA–-like when considering either CL/ML ( = 0.041) or all leishmaniasis patients ( = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA–-like, specifically for Chagas disease samples ( < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML ( = 0.607) or CL ( = 0.205). We recommend ELISA–-rHsp83 as a routine confirmatory serological assay for the diagnosis of infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


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  • Received : 25 Oct 2013
  • Accepted : 23 Jan 2014
  • Published online : 07 May 2014

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