1921
Volume 91, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

To identify the relationship between landscape use and dengue virus (DENV) occurrence in bats, we investigated the presence of DENV from anthropogenically changed and unaltered landscapes in two Biosphere Reserves: Calakmul (Campeche) and Montes Azules (Chiapas) in southern Mexico. Spleen samples of 146 bats, belonging to 16 species, were tested for four DENV serotypes with standard reverse transcriptase polymerase chain reaction (RT-PCR) protocols. Six bats (4.1%) tested positive for DENV-2: four bats in Calakmul (two , one , and one ) and two bats in Montes Azules (both ). No effect of anthropogenic disturbance on the occurrence of DENV was detected; however, all three RT-PCR–positive bat species are considered abundant species in the Neotropics and well-adapted to disturbed habitats. To our knowledge, this study is the first study conducted in southeastern Mexico to identify DENV-2 in bats by a widely accepted RT-PCR protocol. The role that bats play on DENV's ecology remains undetermined.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.13-0524
2014-07-02
2017-11-23
Loading full text...

Full text loading...

/deliver/fulltext/14761645/91/1/129.html?itemId=/content/journals/10.4269/ajtmh.13-0524&mimeType=html&fmt=ahah

References

  1. Mackenzie J, Gubler D, Petersen L, , 2004. Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med 10: S98S109.[Crossref]
  2. World Health Organization, 2013. Sustaining the Drive to Overcome the Global Impact of Neglected Tropical Diseases. Second WHO Report on Neglected Diseases. Geneva: WHO Press, 2529.
  3. Weaver S, Vasilakis N, , 2009. Molecular evolution of dengue viruses: contributions of phylogenetics to understand the history and epidemiology of the preeminent arboviral disease. Infect Genet Evol 9: 523540.[Crossref]
  4. World Health Organization, 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva: WHO Press, 324.
  5. Vasilakis N, Cardosa J, Hanley K, Holmes E, Weaver S, , 2011. Fever from the forest: prospects for the continued emergence of sylvatic dengue virus and its impact on public health. Nat Rev Microbiol 9: 532541.[Crossref]
  6. Roberts D, Peyton E, Pinheiro FP, Balderrama F, Vargas R, , 1984. Associations of arbovirus vectors with gallery forest and domestic environments in southeastern Bolivia. Bull Pan Am Health Organ 18: 337350.
  7. De Thoisy B, Lacoste V, Germain A, Muñoz-Jordán J, Colón C, Mauffrey JF, Delaval M, Catzeflis F, Kasanji M, Matheus S, Dussart P, Morvan J, Aguilar-Setien A, Deparis X, Lavergne A, , 2009. Dengue infection in neotropical forest mammals. Vector Borne Zoonotic Dis 9: 157169.[Crossref]
  8. Calisher C, Holmes K, Domínguez S, Shountz T, Cryan P, , 2008. Bats prove to be rich reservoirs for emerging viruses. Microbe Wash DC 3: 521528.
  9. Turmelle A, Olival K, , 2009. Correlates of viral richness in bats (order Chiroptera). EcoHealth 6: 522539.[Crossref]
  10. Drexler JF, Corman VM, Müller MA, Maganga GD, Vallo P, Binger T, Gloza-Rausch F, Cottontail VM, Rasche A, Yordanov S, Seebens A, Knornschild M, Oppong S, Adu Sarkodie Y, Pongombo C, Lukashev AN, Schmidt-Chanasit J, Stöcker A, Carneiro AJ, Erbar S, Maisnes A, Fornhoffs F, Buettner R, Kalko EK, Kruppa T, Franke CR, Kallies R, Yandoko ER, Herrler G, Reusken C, Hassanin A, Krüger DH, Matthee S, Ulrich RG, Leroy EM, Drosten C, , 2012. Bat host major mammalian paramyxoviruses. Nat Commun 3: 796.[Crossref]
  11. Olival K, Epstein JH, Wang LF, Field H, Daszak P, Aguirre A, Ostfeld R, Daszak P, , 2012. Are bats exceptional viral reservoirs? , eds. New Directions in Conservation Medicine: Applied Cases of Ecological Health, 1st Ed. New York, NY: Oxford University Press, 195212.
  12. Anthony S, Ojeda-Flores R, Rico-Chávez O, Navarrete-Macias I, Zambrana-Torrelio C, Rostal MK, Epstein JH, Tipps T, Liang E, Sanchez-Leon M, Sotomayor-Bonilla J, Aguirre AA, Ávila-Flores R, Medellín RA, Goldstein T, Suzán G, Daszak P, Lipkin WI, , 2013. Coronavirus in bats from Mexico. J Gen Virol 94: 10281038.[Crossref]
  13. O'Connor J, Rowan L, Lawrence J, , 1955. Relationships between the flying fox (genus Pteropus) and arthropod-borne fevers of North Queensland. Nature 176: 472.[Crossref]
  14. Zhang H, Yang X, Li G, : Detection of dengue virus genome RNA in some kinds of animal caught from dengue fever endemic areas in Hainan Island with reverse transcription-polymerase chain reaction. Zhang Shi Yan He Lin Chuang Du Xue Za Zhi 12: 226228.
  15. Platt K, Mangiafico J, Rocha O, Zaldivar M, Mora J, Trueba G, Rowley WA, , 2000. Detection of dengue virus neutralizing antibodies in bats from Costa Rica and Ecuador. J Med Entomol 37: 965967.[Crossref]
  16. Aguilar-Setien A, Romero-Almaraz M, Sánchez-Hernández C, Figueroa R, Juárez-Palma LP, García-Flores MM, Vásquez-Salinas C, Salas-Rojas M, Hidalgo-Martínez AC, Aguilar-Pierlé S, García-Estrada C, Ramos C, , 2008. Dengue virus in Mexican bats. Epidemiol Infect 136: 16781683.[Crossref]
  17. Machain-Williams C, López-Uribe M, Talavera-Aguilar L, Carrillo-Navarrete J, Vera-Escalante L, Puerto-Manzano F, Ulloa A, Farfán-Ale JA, García-Rejón J, Blitvich BJ, Loroño-Pino MA, , 2013. Serologic evidence of flavivirus infection in bats in the Yucatan Peninsula of Mexico. J Wildl Dis 49: 684689.[Crossref]
  18. Medellín R, Arita H, Sánchez O, , 2008. Identificación de los Murciélagos de México. Clave de Identificación de Campo. Mexico City, México: Instituto de Ecología, UNAM, 2878.
  19. Sikes RS, Gannon WL, ACUCASM; , 2011. Guidelines of the American Society of Mammalogists for the use of wild mammals in research. J Mammal 92: 235253.[Crossref]
  20. Lanciotti R, Calisher C, Gubler D, Chang G, Vorndam AV, , 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545551.
  21. Medellín RA, Equihua M, Amin M, , 2000. Bat diversity and abundance as indicators of disturbance in neotropical rainforest. Conserv Biol 14: 16661675.[Crossref]
  22. Vargas J, Medellín RA, Escalona-Segura G, Interián-Sosa L, , 2009. Vegetation complexity and bat-plant dispersal in Calakmul, Mexico. J Nat Hist 43: 219243.[Crossref]
  23. Estrada A, Coates-Estrada R, Meritt D, , 1993. Bat species richness and abundance in a tropical rain forest fragments and in agricultural habitats at Los Tuxtlas, Mexico. Ecography (Cop.) 16: 309318.[Crossref]
  24. R Core Team, 2013. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing.
  25. Gómez Dantes H, , 2007. Elementos económicos y políticos que impactan en el control del dengue en México. Salud Publica Mex 49: 117119.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.13-0524
Loading
/content/journals/10.4269/ajtmh.13-0524
Loading

Data & Media loading...

  • Received : 10 Sep 2013
  • Accepted : 27 Mar 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error