Volume 90, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The zoonotic potential of was studied in an extensive cattle farming region of northern Tunisia. Seventy fecal samples from pre-weaning calves and 403 fecal samples from children were examined by microscopy after modified Ziehl–Neelsen (MZN) staining. Positive specimens were identified at a species level using an 18S rRNA nested polymerase chain reaction (PCR) followed by an Restriction Fragment Length Polymorphism (RFLP) analysis. isolates were subgenotyped by sequence analysis of the glycoprotein 60 (gp60) gene. Among calf samples, 14 samples were positive by MZN method. was identified in all cases. Twelve isolates (85.7%) belonged to family subtype IIa. Subtype IIaA15G2R1 was more prevalent (50%). Two isolates corresponded to the IIdA16G1 subtype. Seven human samples were positive by MZN method. and were identified in four and three cases, respectively. Intraspecific characterization of identified two subtypes, the IIaA15G2R1 and the IIdA16G1, also found in calves.


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  • Received : 10 Sep 2013
  • Accepted : 30 Sep 2013
  • Published online : 05 Feb 2014

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