Volume 90, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



spp. infection and the seroconversion rate in the Amazon have been poorly investigated. This study analyzed individual and household-level risk factors for the presence of IgG antibodies to spp. in urban Amazonian children over a period of 7 years and evaluated the seroconversion rates over a 1-year follow-up. In children < 59 months of age, the overall prevalence rate was 28.08% in 2003 and 23.35% in 2010. The 2010–2011 seroconversion rates were 13.90% for children 6–59 months of age and 12.30% for children 84–143 months of age. Multilevel logistic regression analysis identified child age, previous wheezing, and current infection with hookworm as significant associated factors for spp. seropositivity in 2003. In 2010, age, previous helminthiasis, and having a dog were associated with seropositivity, whereas having piped water inside the household was a protective factor. Control programs mainly need to target at-risk children, water quality control, and animal deworming strategies.


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  1. Santarem VA, Rubinsky-Elefant G, Ferreira MU, Pascucci S, , 2011. Soil-transmitted helminthic zoonoses in humans and associated risk factors. , ed. Soil Contamination. Rijeka, Croatia: InTech, 4366. [Google Scholar]
  2. Choi D, Lim JH, Choi DC, Lee KS, Paik SW, Kim SH, Choi YH, Huh S, , 2012. Transmission of Toxocara canis via ingestion of raw cow liver: a cross-sectional study in healthy adults. Korean J Parasitol 50: 2327.[Crossref] [Google Scholar]
  3. Taylor MR, Keane CT, O'Connor P, Mulvihill E, Holland C, , 1988. The expanded spectrum of toxocaral disease. Lancet 1: 692695.[Crossref] [Google Scholar]
  4. Chieffi PP, Ueda M, Camargo ED, de Souza AM, Guedes ML, Gerbi ML, Spir M, Moreira AS, , 1990. Visceral larva migrans: a seroepidemiological survey in five municipalities of São Paulo state, Brazil. Rev Inst Med Trop Sao Paulo 32: 204210.[Crossref] [Google Scholar]
  5. Overgaauw PA, , 1997. Aspects of Toxocara epidemiology: human toxocarosis. Crit Rev Microbiol 23: 215231.[Crossref] [Google Scholar]
  6. Despommier D, , 2003. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev 16: 265272.[Crossref] [Google Scholar]
  7. Pawlowski Z, , 2001. Toxocariasis in humans: clinical expression and treatment dilemma. J Helminthol 75: 299305.[Crossref] [Google Scholar]
  8. Rubinsky-Elefant G, Hirata CE, Yamamoto JH, Ferreira MU, , 2010. Human toxocariasis: diagnosis, worldwide seroprevalence and clinical expression of the systemic and ocular forms. Ann Trop Med Parasitol 104: 323.[Crossref] [Google Scholar]
  9. Damian MM, Martins M, Sardinha JF, Souza LO, Chaves A, Tavares A de M, , 2007. Frequency of the antibody anti-Toxocara canis in a community along the Uatuma river, State of Amazonas. Rev Soc Bras Med Trop 40: 661664.[Crossref] [Google Scholar]
  10. Matos MF, Melitão DN, Brum MA, Omais M, Quilião ME, Dorval ME, Pereira A da C, Possi LA, Sauer L, Camargo ED, Tundisi RN, , 1997. Presence of anti-Toxocara antibodies in children selected at Hospital Universitário, Campo Grande, MS, Brazil. Rev Inst Med Trop Sao Paulo 39: 4950.[Crossref] [Google Scholar]
  11. Hayashi E, Tuda J, Imada M, Akao N, Fujita K, , 2005. The high prevalence of asymptomatic Toxocara infection among schoolchildren in Manaso, Indonesia. Southeast Asian J Trop Med Public Health 36: 13991406. [Google Scholar]
  12. Thompson DE, Bundy DA, Cooper ES, Schantz PM, , 1986. Epidemiological characteristics of Toxocara canis zoonotic infection of children in a Caribbean community. Bull World Health Organ 64: 283290. [Google Scholar]
  13. Rubinsky-Elefant G, da Silva-Nunes M, Malafronte RS, Muniz PT, Ferreira MU, , 2008. Human toxocariasis in rural Brazilian Amazonia: seroprevalence, risk factors, and spatial distribution. Am J Trop Med Hyg 79: 9398. [Google Scholar]
  14. Ferreira MU, Rubinsky-Elefant G, de Castro TG, Hoffmann EH, da Silva-Nunes M, Cardoso MA, Muniz PT, , 2007. Bottle feeding and exposure to Toxocara as risk factors for wheezing illness among under-five Amazonian children: a population-based cross-sectional study. J Trop Pediatr 53: 119124.[Crossref] [Google Scholar]
  15. Roldán WH, Cavero YA, Espinoza YA, Jiménez S, Gutiérrez CA, , 2010. Human toxocariasis: a seroepidemiological survey in the Amazonian city of Yurimaguas, Peru. Rev Inst Med Trop Sao Paulo 52: 3742.[Crossref] [Google Scholar]
  16. Lynch NR, Eddy K, Hodgen AN, Lopez RI, Turner KJ, , 1988. Seroprevalence of Toxocara canis infection in tropical Venezuela. Trans R Soc Trop Med Hyg 82: 275281.[Crossref] [Google Scholar]
  17. Correa CR, Bismarck CM, , 2010. Toxocariasis: incidence, prevalence and the time serum remains positive in school children from Campinas, SP, Brasil. J Trop Pediatr 56: 215216.[Crossref] [Google Scholar]
  18. Anaruma Filho F, Chieffi PP, Correa CR, Camargo ED, da Silveira EP, Aranha JJ, , 2003. Human toxocariasis: incidence among residents in the outskirts of Campinas, State of São Paulo, Brazil. Rev Inst Med Trop Sao Paulo 45: 293294.[Crossref] [Google Scholar]
  19. Paquet-Durand I, Hernández J, Dolz G, Zuñiqa JJ, Schinieder T, Epe C, , 2007. Prevalence on Toxocara spp., Toxoscaris leonine and ancylostomidae in public parks and beaches in different climate zones of Costa Rica. Acta Trop 104: 3037.[Crossref] [Google Scholar]
  20. Capuano DM, Rocha G de M, , 2005. Environmental contamination by Toxocara spp. eggs in Riberão Preto, São Paulo, State, Brasil. Rev Inst Med Trop Sao Paulo 47: 223226.[Crossref] [Google Scholar]
  21. Fontanarrosa MF, Vezzani D, Basabe J, Eiras DF, , 2006. An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): age, gender, mixed infections, and seasonal and spatial patterns. Vet Parasitol 136: 283295.[Crossref] [Google Scholar]
  22. Instituto Brasileiro de Geografia e Estatística, 2010. Resultados parciais do Estado do Acre no Censo 2010. Available at: http://www.ibge.gov.br/home/estatistica/populacao/censo2010/tabelas_pdf/total_populacao_acre.pdf. Accessed October 10, 2012.
  23. Sistema Estadual de Informações Ambientais: Assis Brasil, Acre. Available at: http://www.seiam.ac.gov.br. Accessed October 10, 2012.
  24. Elefant GR, Shimizu SH, Sanchez MC, Jacob CM, Ferreira AW, , 2006. A serological follow-up of toxocariasis patients after chemotherapy based on the detection of IgG, IgA, and IgE antibodies by enzyme-linked immunosorbent assay. J Clin Lab Anal 20: 164172.[Crossref] [Google Scholar]
  25. Kennedy MW, Maizels RM, Meghji M, Young L, Qureshi F, Smith HV, , 1987. Species-specific and common epitopes on the secreted and surface antigens of Toxocara cati and Toxocara canis infective larvae. Parasite Immunol 9: 407420.[Crossref] [Google Scholar]
  26. Romasanta A, Romero JL, Arias M, Sánchez-Andrade R, López C, Suárez JL, Díaz P, Díez-Baños P, , 2003. Diagnosis of parasitic zoonoses by immunoenzymatic assay-analysis of cross-reactivity among the excretory/secretory antigens of Fasciola hepatica, Toxocara canis, and Ascaris suum . Immunol Invest 32: 131142.[Crossref] [Google Scholar]
  27. Prestes-Carneiro LE, Santarém V, Zago SC, Miguel NA, Zamballi S de F, Villas R, Vaz AJ, Rubinsky-Elefant G, , 2008. Sero-epidemiology of toxocariasis in a rural settlement in São Paulo state, Brazil. Ann Trop Med Parasit 102: 347356.[Crossref] [Google Scholar]
  28. de Souza EA, da Silva-Nunes M, Malafronte R dos S, Muniz PT, Cardoso MA, Ferreira MU, , 2007. Prevalence and spatial distribution of intestinal parasitic infections in a rural Amazonian settlement, Acre State, Brasil. Cad Saude Publica 23: 427434.[Crossref] [Google Scholar]
  29. Gyorkos TW, MacLean JD, Law CG, , 1989. Absence of significant differences in intestinal parasite prevalence estimates after examination of either one or two stool specimens. Am J Epidemiol 130: 976980.[Crossref] [Google Scholar]
  30. Prado MS, Strina A, Barreto ML, Oliveira-Assis AM, Paz LM, Cairncross S, , 2003. Risk factors for infection with Giardia duodenalis in pre-school children in the city of Salvador, Brazil. Epidemiol Infect 131: 899906.[Crossref] [Google Scholar]
  31. Filmer D, Pritchett LH, , 2001. Estimating wealth effects without expenditure data on tears: an application to educational enrollments in states of India. Demography 38: 115132. [Google Scholar]
  32. Muniz PT, Castro TG, Araújo TS, Nunes NB, da Silva-Nunes M, Hoffmann EH, Ferreira MU, Cardoso MA, , 2007. Child health and nutricion in the western Brazilian Amazon: population-based surveys in two counties in Acre State. Cad Saude Publica 23: 12831293.[Crossref] [Google Scholar]
  33. da Silva-Nunes M, Codeço CT, Malafronte RS, da Silva NS, Juncansen C, Muniz PT, Ferreira MU, , 2008. Malaria on the Amazonian frontier: transmission dynamics, risk factors, spatial distribution, and prospects for control. Am J Trop Med Hyg 79: 624635. [Google Scholar]
  34. Campos Júnior D, Elefant GR, de Melo E, Silva EO, Gandolfi L, Jacob CM, Tofeti A, Pratesi R, , 2003. Frequency of seropositivity to Toxocara canis in children of different socioeconomic strata. Rev Soc Bras Med Trop 36: 509513.[Crossref] [Google Scholar]
  35. Muradian V, Gennari SM, Glickman LT, Pinheiro SR, , 2005. Epidemiological aspects of visceral larva migrans in children living at São Remo Community, São Paulo (SP), Brazil. Vet Parasitol 134: 9397.[Crossref] [Google Scholar]
  36. Mendonça LR, Figueiredo CA, Esquivel R, Fiaccone RL, Pontes-de-Carvalho L, Cooper P, Barreto ML, Alcantare-Neves NM, , 2013. Seroprevalence and risk factors for Toxocara infection in children from an urban large setting in northeast Brazil. Acta Trop 128: 9095.[Crossref] [Google Scholar]
  37. Paludo ML, Falavigna DL, Elefant GR, Gomes ML, Baggio ML, Amadei LB, Falavigna-Guilherme AL, , 2007. Frequency of Toxocara infection in children attended by the health public service of Maringá, south Brazil. Rev Inst Med Trop Sao Paulo 49: 343348.[Crossref] [Google Scholar]
  38. Ajayi OO, Duhlinska DD, Agwale SM, Njoku SM, , 2000. Frequency of human toxocariasis in Jos, Plateau State, Nigeria. Mem Inst Oswaldo Cruz 95: 147149.[Crossref] [Google Scholar]
  39. Sharif M, Daryani A, Barzegar G, Nasrolahei M, Khalilian A, , 2010. Seroprevalence of toxocariasis in schoolchildren in northern Iran. Pak J Biol Sci 13: 180184.[Crossref] [Google Scholar]
  40. Sadjjadi SM, Khosravi M, Mehrabani D, Orya A, , 2000. Seroprevalence of Toxocara infection in school children in Shiraz, southern Iran. J Trop Pediatr 43: 327330.[Crossref] [Google Scholar]
  41. Chiodo P, Basualdo J, Ciarmela L, Pezzani B, Apezteguia M, Minvielle M, , 2006. Related factors to human toxocariasis in a rural community of Argentina. Mem Inst Oswaldo Cruz 101: 397400.[Crossref] [Google Scholar]
  42. Alonso JM, Bojanich MV, Chamorro M, Gorodner JO, , 2000. Toxocara seroprevalence in children from a subtropical city in Argentina. Rev Inst Med Trop Sao Paulo 42: 235237.[Crossref] [Google Scholar]
  43. Espinoza Y, Roldán W, Huapaya P, Huiza A, Jiménez S, , 2006. Prevalencia de anticuerpos IgG anti-Toxocara, en pobladores del distrito de Perené. Departamento de Junín. An Fac Med 67 (Suppl 1): S66. [Google Scholar]
  44. Roldán WH, Espinoza YA, Huapaya PE, Huiza AF, Sevilla CR, Jimenéz S, , 2009. Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test. Rev Inst Med Trop Sao Paulo 51: 6771.[Crossref] [Google Scholar]
  45. Baboolal S, Rawlins SC, , 2002. Seroprevalence of toxocariasis in schoolchildren in Trinidad. Trans R Soc Trop Med Hyg 96: 139143.[Crossref] [Google Scholar]
  46. Dermirci M, Kaya S, Cetin E, Aridogan B, Onal S, Korkmaz M, , 2010. Seroepidemiological investigation of toxocariasis in the sparta region of Turkey. Iran J Parasitol 5: 5259. [Google Scholar]
  47. Labruna MB, Pena HF, Souza SL, Pinter A, Silva JC, Ragozo AM, Camargo LM, Gennari SM, , 2006. Prevalência de endoparasitas em cães da área urbana do município de Monte Negro, Rondônia. Arq Inst Biol (Sao Paulo) 73: 183193. [Google Scholar]
  48. Figueiredo SD, Taddei JA, Menezes JJ, Novo NF, Silva EO, Cristóvão HL, Cury MC, , 2005. Clinical-epidemiological study of toxocariasis in a pediatric population. J Pediatr (Rio J) 81: 126132.[Crossref] [Google Scholar]
  49. Teixeira CR, Chieffi PP, Lescano SA, de Melo Silva EO, Fux B, Cury MC, , 2006. Frequency and risk factors for toxocariasis in children from pediatric outpatient center in southeastern Brazil. Rev Inst Med Trop Sao Paulo 48: 251255.[Crossref] [Google Scholar]
  50. Wolfe A, Wright IP, , 2003. Human toxocariasis and direct contact with dogs. Vet Rec 152: 419422.[Crossref] [Google Scholar]
  51. Aydenizöz-Ozkayhan M, Yagci BB, Erat S, , 2008. The investigation of Toxocara canis eggs in coats of different dog breeds as a potential transmission route in human toxocariasis. Vet Parasitol 152: 94100.[Crossref] [Google Scholar]
  52. Roddie G, Stafford P, Holland C, Wolfe A, , 2008. Contamination of dog hair with eggs of Toxocara canis . Vet Parasitol 152: 8593.[Crossref] [Google Scholar]
  53. Overgaauw PA, van Zutphen L, Hoek D, Yaya FO, Roelfsema J, Pinelli E, van Knapen F, Kortbeek LM, , 2009. Zoonotic parasites in fecal samples and fur from dogs and cats in The Netherlands. Vet Parasitol 163: 115122.[Crossref] [Google Scholar]
  54. Agudelo C, Villareal E, Cáceres E, López C, Elijach J, Ramírez N, Hernández C, Corredor A, , 1990. Human and dogs Toxocara canis infection in a poor neighborhood in Bogota. Mem Inst Oswaldo Cruz 85: 7581.[Crossref] [Google Scholar]
  55. Buijs J, Borsboom G, Renting M, Hilgersom WJ, van Wieringen JC, Jansen G, Neijens J, , 1997. Relationship between allergic manifestations and Toxocara seropositivity: a cross-sectional study among elementary school children. Eur Respir J 10: 14671475.[Crossref] [Google Scholar]
  56. Chan PW, Anuar AK, Fong MY, Debruyne JA, Ibrahim J, , 2001. Toxocara seroprevalence and childhood asthma among Malaysian children. Pediatr Int 43: 350353.[Crossref] [Google Scholar]
  57. Cobzaru RG, Rîpâ C, Leon MM, Luca MC, Ivan A, Luca M, , 2012. Correlation between asthma and Toxocara canis infection. Rev Med Chir Soc Med Nat Iasi 116: 727730. [Google Scholar]
  58. Hotez PJ, Wilkins PP, , 2009. Toxocariasis: America's most common neglected infection of poverty and a helminthiasis of global importance? PLoS Negl Trop Dis 3: e400.[Crossref] [Google Scholar]
  59. Dabrowska J, Walski M, Dybicz M, Doligalska M, , 2012. Comparative ultrastructural studies of the alterations to muse lung parenchyma during Trichinella spiralis or Toxocara canis infection. Parasite Immunol 34: 455463.[Crossref] [Google Scholar]
  60. Pinelli E, Withagen C, Fonville M, Verlaan A, Dormans J, van Loveren H, Nicoll G, Maizels RM, van der Giessen J, , 2005. Persistent airway hyper-responsiveness and inflammation of Toxocara canis-infected BALB/c mice. Clin Exp Allergy 35: 826832.[Crossref] [Google Scholar]
  61. Mendonça LR, Veiga RV, Dattoli VC, Figueiredo CA, Fiaccone R, Santos J, Curz ÁA, Rodrigues LC, Cooper PJ, Pontes-de-Carvalho LC, Barreto ML, Alcantara-Neves NM, . 2012. Toxocara seropositivity, atopy and wheezing in children living in poor neighborhoods in urban Latin American. PLoS Negl Trop Dis 6: e1886.[Crossref] [Google Scholar]
  62. Sariego I, Kanobana K, Junco R, Vereecken K, Nuñez FA, Polman K, Bonet M, Rojas L, , 2012. Frequency of antibodies to Toxocara in Cuban schoolchildren. Trop Med Int Health 17: 711714.[Crossref] [Google Scholar]
  63. Ishida MM, Rubinsky-Elefant G, Ferreira AW, Hoshino-Shimizu S, Vaz AJ, , 2003. Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Trop 89: 7384.[Crossref] [Google Scholar]
  64. Jacquier P, Gottstein B, Stingelin Y, Eckert J, , 1991. Immunodiagnosis of toxocarosis in humans: evaluation of a new enzyme-linked immunosorbent assay kit. J Clin Microbiol 29: 18311835. [Google Scholar]
  65. Chieffi PP, Santos SV, Queiroz ML, Lescano SA, , 2009. Human toxocariasis: contribution by Brazilian researchers. Rev Inst Med Trop Sao Paulo 51: 301308.[Crossref] [Google Scholar]
  66. Smith HV, Lewis JW, Maizels RM, , 1993. Antibody reactivity in human Toxocariasis. , eds. Toxocara and Toxocariasis: Clinical, Epidemiological and Molecular Perspectives. London: British Society for Parasitology, Inc., 91109. [Google Scholar]
  67. Fillaux J, Magnaval JF, , 2013. Laboratory diagnosis of human toxocariasis. Vet Parasitol 193: 327336.[Crossref] [Google Scholar]
  68. Lopez M de L, Martin G, Chamorro M del C, Mario Alonso J, , 2005. Toxocariasis in children from a subtropical region. Medicina (B Aires) 65: 226230. [Google Scholar]

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  • Received : 02 Sep 2013
  • Accepted : 05 Jan 2014
  • Published online : 02 Apr 2014

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