1921
Volume 90, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Large-scale epidemiological surveillance of dengue in the field and dengue patient management require simple methods for sample collection, storage, and transportation as well as effective diagnostic tools. We evaluated the kinetics of three biological markers of dengue infection—non-structural protein 1 (NS1) antigen, immunoglobulin M (IgM), and IgA—in sequential capillary blood samples collected from fingertips of confirmed dengue patients. The overall sensitivities and specificities of the tests were 96% and 100%, respectively, for NS1, 58.1% and 100%, respectively, for IgM, and 33% and 100%, respectively, for IgA. During the acute phase of the disease, NS1 was the best marker of dengue infection, with a sensitivity of 98.7%, whereas from day 5, all three markers exhibited relevant levels of sensitivity. This first descriptive study of the kinetics of biological markers of dengue in capillary blood samples confirms the usefulness of this biological compartment for dengue diagnosis and argues for its exploitation in community-level and remote settings.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.13-0458
2014-03-05
2017-11-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/90/3/438.html?itemId=/content/journals/10.4269/ajtmh.13-0458&mimeType=html&fmt=ahah

References

  1. Monath TP, , 1994. Dengue: the risk to developed and developing countries. Proc Natl Acad Sci USA 91: 23952400.[Crossref]
  2. Halstead SB, , 2007. Dengue. Lancet 370: 16441652.[Crossref]
  3. Kyle J, Harris E, , 2008. Global spread and persistence. Annu Rev Microbiol 62: 7192.[Crossref]
  4. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV, , 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545551.
  5. Henchal EA, McCown JM, Seguin MC, Gentry MK, Brandt WE, , 1983. Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluorescence assay. Am J Trop Med Hyg 32: 164169.
  6. Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M, , 2002. Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40: 376381.[Crossref]
  7. Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, Storck-Herrmann C, Cesaire R, Morvan J, Flamand M, Baril L, , 2006. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol 13: 11851189.[Crossref]
  8. Kumarasamy V, Chua SK, Hassan Z, Wahab AH, Chem YK, Mohamad M, Chua KB, , 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods 140: 7579.[Crossref]
  9. Vazquez S, Ruiz D, Barrero R, Ramirez R, Calzada N, del Rosario Peña B, Reyes S, Guzman MG, , 2010. Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. Diagn Microbiol Infect Dis 68: 4649.[Crossref]
  10. Kuno G, Gomez I, Gubler DJ, , 1991. An ELISA procedure for the diagnosis of dengue infections. J Virol Methods 33: 101113.[Crossref]
  11. Talarmin A, Labeau B, Lelarge J, Sarthou JL, , 1998. Immunoglobulin A-specific capture enzyme-linked immunosorbent assay for diagnosis of dengue fever. J Clin Microbiol 36: 11891192.
  12. Balmaseda A, Guzmán MG, Hammond S, Robleto G, Flores C, Téllez Y, Videa E, Saborio S, Pérez L, Sandoval E, Rodriguez Y, Harris E, , 2003. Diagnosis of dengue virus infection by detection of specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva. Clin Diagn Lab Immunol 10: 317322.
  13. Nawa M, Takasaki T, Ito M, Inoue S, Morita K, Kurane I, , 2005. Immunoglobulin A antibody responses in dengue patients: a useful marker for serodiagnosis of dengue virus infection. Clin Diagn Lab Immunol 12: 12351237.
  14. Matheus S, Meynard JB, Lacoste V, Morvan J, Deparis X, , 2007. Use of capillary blood samples as a new approach for diagnosis of dengue virus infection. J Clin Microbiol 45: 887890.[Crossref]
  15. Vázquez S, Cabezas S, Pérez AB, Pupo M, Ruiz D, Calzada N, Bernardo L, Castro O, González D, Serrano T, Sanchez A, Guzmán MG, , 2007. Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. Int J Infect Dis 11: 256262.[Crossref]
  16. Balmaseda A, Saborio S, Tellez Y, Mercado JC, Pérez L, Hammond SN, Rocha C, Kuan G, Harris E, , 2008. Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies. J Clin Virol 43: 287291.[Crossref]
  17. Anders KL, Nguyet NM, Quyen NT, Ngoc TV, Tram TV, Gan TT, Tung NT, Dung NT, Chau NV, Wills B, Simmons CP, , 2012. An evaluation of dried blood spots and oral swabs as alternative specimens for the diagnosis of dengue and screening for past dengue virus exposure. Am J Trop Med Hyg 87: 165170.[Crossref]
  18. Matheus S, Meynard JB, Lavergne A, Girod R, Moua D, Labeau B, Dussart P, Lacoste V, Deparis X, , 2008. Dengue-3 outbreak in Paraguay: investigations using capillary blood samples on filter paper. Am J Trop Med Hyg 79: 685687.
  19. Matheus S, Deparis X, Labeau B, Lelarge J, Morvan J, Dussart P, , 2005. Discrimination between primary and secondary dengue virus infection by an immunoglobulin G avidity test using a single acute-phase serum sample. J Clin Microbiol 43: 27932797.[Crossref]
  20. World Health Organization, 2009. Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. New edition. Geneva: World Health Organization.
  21. Kumarasamy V, Chua SK, Hassan Z, Wahab AH, Chem YK, Mohamad M, Chua KB, , 2007. Evaluating the sensitivity of a commercial dengue NS1 antigen-capture ELISA for early diagnosis of acute dengue virus infection. Singapore Med J 48: 669673.
  22. Chuansumrit A, Chaiyaratana W, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, , 2008. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J 27: 4348.[Crossref]
  23. Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, , 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.[Crossref]
  24. McBride WJ, , 2009. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis 64: 3136.[Crossref]
  25. Guzman MG, Jaenisch T, Gaczkowski R, Ty Hang VT, Sekoran SD, Kroeger A, Vazquez S, Ruiz D, Martinez E, Mercado JC, Balmaseda A, Harris E, Dimano E, Leano PS, Yoksan S, Villegas E, Benduzu H, Villalobos I, Farrar J, Simmons CP, , 2010. Multi-country evaluation of the sensitivity and specificity of two commercially available NS1 ELISA assays for dengue diagnosis. PLoS Negl Trop Dis 4: e811.[Crossref]
  26. Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B, , 2010. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J 7: 361.[Crossref]
  27. Blacksell SD, Mammen MP, Jr Thongpaseuth S, Gibbons RV, Jarman RG, Jenjaroen K, Nisalak A, Phetsouvanh R, Newton PN, Day NP, , 2008. Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis 60: 4349.[Crossref]
  28. Lapphra K, Sangcharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T, Yoksan S, Wattanamongkolsil L, Thamlikitkul V, , 2008. Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis 60: 387391.[Crossref]
  29. Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP, , 2009. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3: e360.[Crossref]
  30. Castro-Jorge LA, Machado PR, Fávero CA, Borges MC, Passos LM, , 2010. Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil. J Med Virol 82: 14001405.[Crossref]
  31. Kumarasamy V, Wahab AHA, Chuab SK, Hassan ZA, Chema YK, Mohamada M, Chua KB, , 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acutedengue virus infection. J Virol Methods 140: 7579.[Crossref]
  32. Avirutnan P, Zhang L, Punyadee N, Manuyakorn A, Puttikhunt C, Kasinrerk W, Malasit P, Atkinson JP, Diamond MS, , 2007. Secreted NS1 of dengue virus attaches to the surface of cells via interactions with heparan sulfate and chondroitin sulfate E. PLoS Pathog 3: e183.[Crossref]
  33. Huang CH, Kuo LL, Yang KD, Lin PS, Lu PL, Lin CC, Chang K, Chen TC, Lin WR, Lin CY, Chen YH, Wu HS, , 2013. Laboratory diagnostics of dengue fever: an emphasis on the role of commercial dengue virus nonstructural protein 1 antigen rapid test. J Microbiol Immunol Infect 46: 358365.[Crossref]
  34. de la Cruz Hernández SI, González Mateos S, Flores Aguilar H, López Martinez I, Alpuche Aranda C, Ludert JE, Del Angel RM, , 2012. Evaluation of a novel commercial rapid test for dengue diagnosis based on specific IgA detection. Diagn Microbiol Infect Dis 72: 150155.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.13-0458
Loading
/content/journals/10.4269/ajtmh.13-0458
Loading

Data & Media loading...

  • Received : 05 Aug 2013
  • Accepted : 05 Dec 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error