Volume 90, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Thirty pools from 900 (540 females and 360 males) sandflies collected during the summer of 2012 in the Fermo area (Marche Region, central Italy) were tested for the presence of Phleboviruses. A nested polymerase chain reaction was performed using degenerated primers amplifying a fragment of the polymerase gene (large segment) and a fragment of the nucleoprotein gene (small segment) of the genus . One pool was positive for Toscana virus, as expected from results of studies in the area, and six pools were positive for a putative novel Phlebovirus. Virus isolation in Vero cells was performed. Minimum field infection rates/1,000 insects processed for the novel and Toscana viruses were 6.7 and 1.0, respectively. Phylogenetic analysis of the novel Phlebovirus, tentatively named Fermo virus, placed it in the Sandfly Fever Naples virus serocomplex.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Maroli M, Feliciangeli D, Bichaud L, Charrel RN, Gradoni L, 2013. Phlebotomine sandflies and spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol 27: 123147.[Crossref]
    [Google Scholar]
  2. King AM, Adams MJ, Caestens EB, Lefkowitz EJ, 2012. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Amsterdam: Elsevier.
    [Google Scholar]
  3. Depaquit J, Grandadam M, Fouque F, Andry PE, Peyrefitte C, 2010. Arthropod-borne viruses transmitted by Phletomine sandflies in Europe: a review. Euro Surveill 15: 19507.
    [Google Scholar]
  4. Ciufolini MG, Maroli M, Miceli N, Nicoletti L, Cecchetti S, Amore R, Percopo S, Verani P, 1990. Monitoring of Phlebotominae sandflies transmitted viruses activity in Marche region (Italy). Arbo Info Exch 5859.
    [Google Scholar]
  5. Charrel RN, Gallian P, Navarro-Mari J-M, Nicoletti L, Papa A, Sánchez-Seco MP, Tenorio A, de Lamballerie X, 2005. Emergence of Toscana virus in Europe. Emerg Infect Dis 1: 16571663.[Crossref]
    [Google Scholar]
  6. Venturi G, Madeddu G, Rezza G, Ciccozzi M, Pettinato ML, Cilliano M, Fiorentini C, Mura MS, Ciufolini MG, 2007. Detection of Toscana virus central nervous system infections in Sardinia Island, Italy. J Clin Virol 40: 9091.[Crossref]
    [Google Scholar]
  7. Theodor O, 1958. Psychodidae-Phlebotomine. Lindner E, ed. Die Fliegen der Palaearktischen Region, 9c. Stuttgart, Germany: Schweizerbart'sche Verlagsbuchhandlung, 155.
    [Google Scholar]
  8. Léger N, Pesson B, Madulo-Leblond G, Abonnenc E, 1983. Differentiation of females of the subgenus Larroussius Nitzulescu 1931 (Diptera-Phlebotomidae) of the Mediterranean region. Ann Parasitol Hum Comp 58: 611623.
    [Google Scholar]
  9. Charrel RN, Izri A, Temmam S, Delaunay P, Toga I, Dumon H, Marty P, de Lamballerie X, Parola P, 2007. Cocirculation of 2 genotypes of Toscana virus, southeastern France. Emerg Infect Dis 13: 465468.[Crossref]
    [Google Scholar]
  10. Sánchez-Seco MP, Echevarría JM, Hernández L, Estévez D, Navarro-Marí JM, Tenorio AJ, 2003. Detection and identification of Toscana and other phleboviruses by RT-nested-PCR assays with degenerated primers. Med Virol 71: 140149.[Crossref]
    [Google Scholar]
  11. Verani P, Ciufolini MG, Caciolli S, Renzi A, Nicoletti L, Sabatinelli G, Bartolozzi D, Volpi G, Amaducci L, Coluzzi M, 1988. Ecology of viruses isolated from sand flies in Italy and characterized of a new Phlebovirus (Arbia virus). Am J Trop Med Hyg 38: 433439.
    [Google Scholar]
  12. Ciufolini MG, Maroli M, Guandalini E, Marchi A, Verani P, 1989. Experimental studies on the maintenance of Toscana and Arbia viruses (Bunyaviridae: Phlebovirus). Am J Trop Med Hyg 40: 669675.
    [Google Scholar]

Data & Media loading...

Erratum to Figure 2 legend

  • Received : 05 Aug 2013
  • Accepted : 19 Dec 2013
  • Published online : 02 Apr 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error